basic biology

Basic Biology

advanced biology

Advanced Biology

clinical skills

Clinical Skills

chemistry

Chemistry

environmental sciences

Environmental Sciences

physics

Physics

engineering

Engineering

psychology

Psychology

jove science education

JoVE Science Education

A video library dedicated to teaching the practice and theory of scientific experiments through engaging and easy-to-understand visual demonstrations.

Education

flow cytometry and fluorescence-activated cell sorting (facs): isolation of splenic b lymphocytes

flow-cytometry-fluorescence-activated-cell-sorting-facs-isolation

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3
1 Unit for Lymphopoiesis, Department of Immunology, Pasteur ...

magnetic activated cell sorting (macs): isolation of thymic t lymphocytes

magnetic-activated-cell-sorting-macs-isolation-of-thymic-t-lymphocytes

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3
1 Unit for Lymphopoiesis, Department of Immunology, Pasteur ...

elisa assays: indirect, sandwich, and competitive

elisa-assays-indirect-sandwich-and-competitive

ELISA Assays: Indirect, Sandwich, and Competitive

Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4
1 Department of Urology, University of Minnesota, Minneapolis, MN 55455
...

elispot assay: detection of ifn-&#947; secreting splenocytes

elispot-assay-detection-of-ifn-secreting-splenocytes

ELISPOT Assay: Detection of IFN-&#947; Secreting Splenocytes

Source: Tonya J. Webb1
1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum ...

immunohistochemistry and immunocytochemistry: tissue imaging via light microscopy

immunohistochemistry-immunocytochemistry-tissue-imaging-via-light

Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy

Source: Michael S. Lee1 and Tonya J. Webb1
1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and ...

antibody generation: producing monoclonal antibodies using hybridomas

antibody-generation-producing-monoclonal-antibodies-using-hybridomas

Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas

Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4
1 Microbiology, Immunology, and Cancer Biology Graduate Program, ...

immunofluorescence microscopy: immunofluorescence staining of paraffin-embedded tissue sections

immunofluorescence-microscopy-immunofluorescence-staining-paraffin

Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections

Source: Thomas Chaffee1, Thomas S. Griffith2,3,4, and Kathryn L. Schwertfeger1,3,4
1 Department of Lab Medicine and Pathology, University of Minnesota, ...

confocal fluorescence microscopy: a technique to determine the localization of proteins in mouse fibroblasts

confocal-fluorescence-microscopy-technique-to-determine-localization

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

Source: Dominique R. Bollino1, Eric A. Legenzov2, Tonya J. Webb1
1 Department of Microbiology and Immunology, University of Maryland School of Medicine ...

immunoprecipitation-based techniques: purification of endogenous proteins using agarose beads

immunoprecipitation-based-techniques-purification-endogenous-proteins

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

Source: Susannah C. Shissler1, Tonya J. Webb1
1 Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201
Immunoprecipitation ...

cell cycle analysis: assessing cd4 and cd8 t cell proliferation after stimulation using cfse staining and flow cytometry

cell-cycle-analysis-assessing-cd4-cd8-t-cell-proliferation-after

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3
1 Unit for Lymphpoiesis, Department of Immunology, Pasteur ...

adoptive cell transfer: introducing donor mouse splenocytes to a host mouse and assessing success via facs

adoptive-cell-transfer-introducing-donor-mouse-splenocytes-to-host

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

assay for cell death: chromium release assay of cytotoxic ability

assay-for-cell-death-chromium-release-assay-of-cytotoxic-ability

Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability

immunology

Immunology

This collection covers many staple techniques of immunology labs, including the labeling and sorting of immune cells. It also demonstrates proliferation methods for immune cells and antibodies, as well as common assays for immune activity including ELISA. Finally, it demonstrates staining and imaging of immune tissue and cell samples.