نشكر الدكتور راي Krishanu للأسهم ذبابة الفاكهة، Tarjani أغاروال للحفاظ على قفص ذبابة الفاكهة، بيتر Juo لnuIs25 وCGC لC. ايليجانس السلالات. أدلى SPK jsIs609 في المختبر مايكل Nonet ل. نشكر آربان أجنيهوتري (BITS بيلاني) لمساعدته في الوقت الفاصل بين التصوير النقل الميتوكوندريا من الحيوانات jsIs609 في أجهزة ميكروفلويديك. ونحن ممتنون للدكتور براكاش Vatsala كرومالاي وSURYA لتزويدنا الأجنة الزرد. نشكر الدكتور كريشنا والمكاتب المركزية الوطنية في CIFF لاستخدام المجهر القرص الغزل مبائر بدعم من وزارة العلوم والتكنولوجيا مركز لتقنية النانو-(رقم SR/55/NM-36-2005). نشكر أيضا Kaustubh راو، فينكاترامان V. وSachidanand Chetana للمناقشات. وقد تم تمويل هذا العمل من قبل الزمالة ما بعد الدكتوراه DBT (SM)، DST المسار السريع مخطط (SM) ومنحة لDBT (SPK). وأيد SA من المنح وDST CSIR لSPK.

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الإعلان عن أي تضارب في المصالح.

PDMS الأجهزة ميكروفلويديك شفافة بصريا بالتالي يمكن استخدامها لتصوير بجودة عالية في الجسم الحي من أي كائن نموذج شفاف / شفافة. لدينا تصميم مناسب لتصوير عالية المكانية والزمانية التكبير من الأحداث الخلوية وشبه الخلوية في الحيوانات الحية سليمة. باستخدام تقنيات الطب?...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> اسم كاشف </td><td> شركة </td><td> كتالوج رقم </td><td> التعليقات (اختياري) </td></tr><tr><td> رقائق السيليكون </td><td> جامعة رقاقة </td><td> 150 مم (100) الصف الميكانيكية SSP سي </td><td></td></tr><tr><td> Clewin البرمجيات </td><td> WieWeb البرمجيات </td><td> الإصدار 2.90 </td><td></td></tr><tr><td> الليزر الراسمة </td><td> غرامة الخط التصوير </td><td> 65024 DPI </td><td></td></tr><tr><td> HMDS </td><td> سيغما الدريخ </td><td> 440191-100ML </td><td></td></tr><tr><td> SU8 </td><td> Microchem </td><td> SU8-2025، SU8-2050 </td><td></td></tr><tr><td> المطور </td><td> Microchem </td><td> SU8 المطور </td><td></td></tr><tr><td> سيلاني </td><td> سيغما الدريخ </td><td> 448931-10G </td><td></td></tr><tr><td> PDMS </td><td> داو كورنينج </td><td> Sylgard 184 </td><td></td></tr><tr><td> UV مصباح </td><td> مشربية نافذة ناتئة </td><td> 66943 </td><td> 200W الزئبق اوريل الخفيفة </td></tr><tr><td> فرن الهواء الساخن </td><td> آلات فائقة </td><td> والعرف </td><td> وضعت في 50 ° C </td></tr><tr><td> لوحة الساخن </td><td> IKA مختبر معدات </td><td> 3810000 </td><td> <a href="http://www.ika.com" target="_blank">http://www.ika.com</a> </td></tr><tr><td> البلازما الأنظف </td><td> Harrick البلازما </td><td> PDC-32G </td><td></td></tr><tr><td> الغزال </td><td> نظم انتاج اشباه الموصلات </td><td> SPIN150-NPP </td><td> <a href="http://www.SPS-Europe.com" target="_blank">www.SPS-Europe.com</a> </td></tr><tr><td> زجاج غطاء زلة </td><td> الذهب خاتم </td><td> 22 × 22 مم، سماكة رقم 1 </td><td></td></tr><tr><td>C. ايليجانس </td><td> Caenorhabditis علم الوراثة مركز (CGC) </td><td> e1265، ayIs4 </td><td></td></tr><tr><td> ذبابة الفاكهة </td><td> بلومنجتون </td><td> P {} chaGAL4 / cyo، UAS، syt.eGFP </td><td></td></tr><tr><td> الزرد </td><td> نوع البرية الهندية </td><td> نوع البرية </td><td></td></tr><tr><td> Tygon أنبوب </td><td> سيجما </td><td> Z279803 </td><td></td></tr><tr><td> الدقيقة إبرة </td><td> سيجما </td><td> Z118044 </td><td> مقطعة إلى قطع 1 سم </td></tr><tr><td> 3-الطريقة محبس </td><td> سيجما </td><td> S7521 </td><td></td></tr><tr><td> هاريس الناخس </td><td> سيجما </td><td> Z708631 </td><td></td></tr><tr><td> مضغوط النيتروجين الغاز </td><td> الغاز المورد المحلي </td><td></td><td> استخدام منظم للسيطرة على ضغط </td></tr><tr><td> ستيريو المجهر &lt;/ TD&gt; <td> نيكون </td><td> SMZ645 </td><td></td></tr><tr><td> مبائر المجهر </td><td> ANDOR وأوليمبوس </td><td> يوكوجاوا الغزل مبائر المجهر القرص </td><td></td></tr><tr><td> يماغيج </td><td> المعاهد الوطنية للصحة </td><td> <a href="http://www.rsbweb.nih.gov/ij" target="_blank">www.rsbweb.nih.gov / ط</a> </td><td> جافا برنامج يستند معالجة الصور </td></tr></tbody></table>

simple microfluidic devices for in vivo imaging of c. elegans, drosophila and zebrafish

مايكرو الأجهزة fluidic ملفقة توفير الوصول الجزئي للبيئة في الدراسات المجراة على الكائنات الصغيرة. عمليات تصنيع بسيطة متوفرة لأجهزة ميكروفلويديك باستخدام تقنيات الطباعة الحجرية الناعمة 1-3. وقد استخدمت أجهزة ميكروفلويديك لشبه الخلوية التصوير 4،5، في الجسم الحي ليزر 2،6 المجهرية والخلوية التصوير 4،7. في الجسم الحي التصوير يتطلب تجميد الكائنات الحية. وقد تحقق ذلك باستخدام الشفط 5،8، 6،7،9 قنوات مدبب، الأغشية تشوه 2-4،10، مع شفط تبريد إضافية 5، مخدر الغاز 11، درجة حرارة المواد الهلامية الحساسة 12، 13 و الغراء CYANOACRYLATE التخدير مثل الليفاميزول 14 ، 15. يشيع استخدام التخدير تؤثر انتقال متشابك و16،17 ومن المعروف أن يكون لها آثار ضارة على شبه الخلوية 4 النقل العصبية. في هذا شudy نظهر غشاء بولي ميثيل يعتمد-siloxane (PDMS) الجهاز الذي يسمح مخدر تجميد سليمة خالية من الكائنات نموذج الوراثية مثل ايليجانس Caenorhabditis (C. ايليجانس)، ذبابة الفاكهة اليرقات واليرقات الزرد. هذه الكائنات هي مناسبة لنموذج من الدراسات المجراة في أجهزة ميكروفلويديك بسبب صغر أقطار والهيئات شفافة بصريا أو شفافة. بأقطار تتراوح من الجسم ميكرومتر ميكرومتر إلى 10 ~ 800 ~ للمراحل الأولى من اليرقات C. ايليجانس والزرد اليرقات وتتطلب الأجهزة ميكروفلويديك من مختلف الأحجام لتحقيق الشلل الكامل لارتفاع الوقت الفاصل بين التصوير القرار. وثبتوا هذه الكائنات باستخدام الضغوط التي مارستها غاز النيتروجين المضغوط من خلال عمود السائل وتصويرها باستخدام مجهر مقلوب. صدر الحيوانات من مصيدة العودة إلى طبيعته في غضون تنقل 10 دقيقة. علينا أن نبرهن أربعة طلبات من الوقت الفاصل بين التصوير في C. ايليجانسوهي التصوير النقل الميتوكوندريا في الخلايا العصبية، ما قبل متشابك الحويصلة النقل في النقل عيب متحولة والنقل مستقبلات الغلوتامات وس انقسام الخلايا أرومة عصبية. البيانات التي تم الحصول عليها من مثل هذه الأفلام تظهر أن تجميد ميكروفلويديك هو وسيلة مفيدة ودقيقة في الحصول على البيانات الجسم الحي للأحداث الخلوية وشبه الخلوية بالمقارنة مع الحيوانات تخدير (الشكل 3C 1J و 4-F). تم تغيير أبعاد الجهاز لإتاحة الوقت الفاصل بين التصوير من مراحل مختلفة من C. ايليجانس، أولا يرقات ذبابة الفاكهة واليرقات الزرد الطور. نقل الحويصلات التي تحمل علامة synaptotagmin ذات الكلمات الدلالية مع GFP (syt.eGFP) في الخلايا العصبية الحسية تظهر الحركة الموجهة من علامات حويصلة متشابك المعبر عنها في الخلايا العصبية الحسية سليمة العاملات في 1 يرقات ذبابة الفاكهة الطور. وقد استخدم جهاز مماثل لتنفيذ الوقت الفاصل بين التصوير من نبضات القلب خلال 30 ساعة ~ الإخصاب آخر (HPF)الزرد اليرقات. هذه البيانات تظهر أن يمكن تطبيق أجهزة بسيطة وضعنا لمجموعة متنوعة من نظم نموذجية لدراسة الظواهر البيولوجية والخلية عدة التنموية في الجسم الحي.

الجهاز هو شل حركة كتلة PDMS طبقة ثنائية ملفقة من قبل الرابطة طبقتين: طبقة تدفق (طبقة 1) وطبقة التحكم (طبقة 2) كما هو موضح في الشكل 1. توصيل فخ الرئيسي لاسطوانة غاز النيتروجين من خلال تنظيم وقفة الديك 3-سيلة لتطبيق اللازمة (3-14 رطل) الضغط على الغشاء من خلال عمود السائل...

Watch this Scientific Journal Video about Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish at JoVE.com

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

وقد تم تطوير جهاز بسيط ميكروفلويديك لأداء مخدر مجانا<em&gt; في الجسم الحي</em&gt; تصوير<em&gt; C. ايليجانس</em&gt;، سليمة<em&gt; ذبابة الفاكهة</em&gt; اليرقات واليرقات الزرد. الجهاز يستخدم غشاء PDMS تشوه في شل هذه الكائنات نموذج لأداء التصوير مرور الوقت عمليات عديدة مثل ضربات القلب، انقسام الخلايا وشبه الخلوية النقل العصبية. نحن لشرح استخدام هذا الجهاز، وتظهر أمثلة على أنواع مختلفة من البيانات التي تم جمعها من أنظمة نموذجا مختلفا.

ميكروفلويديك أجهزة بسيطة لل<em&gt; في الجسم الحي</em&gt; التصوير من<em&gt; C. ايليجانس</em&gt;،<em&gt; ذبابة الفاكهة</em&gt; واسماك الزرد

Micro fabricated fluidic devices provide an accessible micro-environment for in vivo studies on small organisms. Simple fabrication processes are ...

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Research

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Bioengineering

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

17.0K Views.  NCBS-TIFR. وقد تم تطوير جهاز بسيط ميكروفلويديك لأداء مخدر مجانا في الجسم الحي تصوير C. ايليجانس، سليمة ذبابة الفاكهة اليرقات واليرقات الزرد. الجهاز يستخدم غشاء PDMS تشوه في شل هذه الكائنات نموذج لأداء التصوير مرور الوقت عمليات عديدة مثل ضربات القلب، ...

Video: Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting and counting cells. However, manipulating the cell within the fluidic domain remains a challenge and requires complicated fabrication protocols for forming valves and electrodes, or demands specialty equipment like optical tweezers. Here, we demonstrate that conventional printed circuit boards (PCB) can be used for the non-contact manipulation of cells by employing dielectrophoresis (DEP) for bead and cell manipulation in laminar flow fields for bioactuation, and for cell and bead separation in multichannel microfluidic devices. First, we present the protocol for assembling the DEP electrodes and microfluidic devices, and preparing the cells for DEP. Then, we characterize the DEP operation with polystyrene beads. Lastly, we show representative results of bead and cell separation in a multichannel microfluidic device. In summary, DEP is an effective method for manipulating particles (beads or cells) within microfluidic devices.

Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.

الخرز وفصل الخلايا في أجهزة ميكروفلويديك متعدد القنوات باستخدام Dielectrophoresis وتدفق رقائقي

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting ...

A Microfluidic Technique to Probe Cell Deformability

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an efficient manner. Typically, data for ~102 cells can be acquired within a 1 hr experiment. An automated image analysis program enables efficient post-experiment analysis of image data, enabling processing to be complete within a few hours. Our device geometry is unique in that cells must deform through a series of micron-scale constrictions, thereby enabling the initial deformation and time-dependent relaxation of individual cells to be assayed. The applicability of this method to human promyelocytic leukemia (HL-60) cells is demonstrated. Driving cells to deform through micron-scale constrictions using pressure-driven flow, we observe that human promyelocytic (HL-60) cells momentarily occlude the first constriction for a median time of 9.3 msec before passaging more quickly through the subsequent constrictions with a median transit time of 4.0 msec per constriction. By contrast, all-trans retinoic acid-treated (neutrophil-type) HL-60 cells occlude the first constriction for only 4.3 msec before passaging through the subsequent constrictions with a median transit time of 3.3 msec. This method can provide insight into the viscoelastic nature of cells, and ultimately reveal the molecular origins of this behavior.

We demonstrate a microfluidics-based assay to measure the timescale for cells to transit through a sequence of micron-scale constrictions.

تقنية ميكروفلويديك للتحقيق التشوه خلية

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an ...

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Although microfluidics provides exquisite control of the cellular microenvironment, culturing cells within microfluidic devices can be challenging. 3D culture of cells in collagen type I gels helps to stabilize cell morphology and function, which is necessary for creating microfluidic tissue models in microdevices. Translating traditional 3D culture techniques for tissue culture plates to microfluidic devices is often difficult because of the limited channel dimensions. In this method, we describe a technique for modifying native type I collagen to generate polycationic and polyanionic collagen solutions that can be used with layer-by-layer deposition to create ultrathin collagen assemblies on top of cells cultured in microfluidic devices. These thin collagen layers stabilize cell morphology and function, as shown using primary hepatocytes as an example cell, allowing for the long term culture of microtissues in microfluidic devices.

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

طبقة تلو طبقة الكولاجين ترسب في أجهزة ميكروفلويديك لMicrotissue لتحقيق الاستقرار

Although microfluidics provides exquisite control of the cellular microenvironment, culturing cells within microfluidic devices can be challenging. 3D ...

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

The behavior of directional cell migration under a direct current electric-field (dcEF) is referred to as electrotaxis. The significant role of physiological dcEF in guiding cell movement during embryo development, cell differentiation, and wound healing has been demonstrated in many studies. By applying microfluidic chips to an electrotaxis assay, the investigation process is shortened and experimental errors are minimized. In recent years, microfluidic devices made of polymeric substances (e.g., polymethylmethacrylate, PMMA, or acrylic) or polydimethylsiloxane (PDMS) have been widely used in studying the responses of cells to electrical stimulation. However, unlike the numerous steps required to fabricate a PDMS device, the simple and rapid construction of the acrylic micro&#64258;uidic chip makes it suitable for both device prototyping and production. Yet none of the reported devices facilitate the efficient study of the simultaneous chemical and dcEF effects on cells. In this report, we describe our design and fabrication of an acrylic-based multichannel dual-electric-field (MDF) chip to investigate the concurrent effect of chemical and electrical stimulation on lung cancer cells. The MDF chip provides eight combinations of electrical/chemical stimulations in a single test. The chip not only greatly shortens the required experimental time but also increases accuracy in electrotaxis studies.

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.

الدراسات انجذاب كهربي من الرئة خلايا سرطان باستخدام القنوات المتعددة ثنائي الكهربائية الميدان ميكروفلويديك رقاقة

The behavior of directional cell migration under a direct current electric-field (dcEF) is referred to as electrotaxis. The significant role of ...

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol adhesive through a metal stencil, the overall amount of adhesive used in assembling paper microfluidic devices can be significantly reduced. We show on a simple 4-layer planar paper microfluidic device that the optimal adhesive application technique and device construction style depends heavily on desired performance characteristics. By moderately increasing the overall area of a device, it is possible to dramatically decrease the wicking time and increase device success rates while also reducing the amount of adhesive required to keep the device together. Such adhesive application also causes the adhesive to form semi-permanent bonds instead of permanent bonds between paper layers, enabling single-use devices to be non-destructively disassembled after use. Nonplanar 3D origami devices also benefit from the semi-permanent bonds during folding, as it reduces the likelihood that unrelated faces may accidently stick together. Like planar devices, nonplanar structures see reduced wicking times with patterned adhesive application vs uniformly applied adhesive.

We demonstrate the use of patterned aerosol adhesives to construct 3D paper microfluidic devices. This method of adhesive application forms semi-permanent bonds between layers, enabling single-use devices to be non-destructively disassembled after use and to ease folding complex nonplanar structures.

باستخدام لاصق الزخرفة لانشاء 3D ورقة أجهزة ميكروفلويديك

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to entry for non-specialists who would benefit greatly from the ability to develop their own microfluidic devices to address research questions. Particularly lacking has been the open dissemination of protocols related to photolithography, a key step in the development of a replica mold for the manufacture of polydimethylsiloxane (PDMS) devices. While the fabrication of single height silicon masters has been explored extensively in literature, fabrication steps for more complicated photolithography features necessary for many interesting device functionalities (such as feature rounding to make valve structures, multi-height single-mold patterning, or high aspect ratio definition) are often not explicitly outlined. 
Here, we provide a complete protocol for making multilayer microfluidic devices with valves and complex multi-height geometries, tunable for any application. These fabrication procedures are presented in the context of a microfluidic hydrogel bead synthesizer and demonstrate the production of droplets containing polyethylene glycol (PEG diacrylate) and a photoinitiator that can be polymerized into solid beads. This protocol and accompanying discussion provide a foundation of design principles and fabrication methods that enables development of a wide variety of microfluidic devices. The details included here should allow non-specialists to design and fabricate novel devices, thereby bringing a host of recently developed technologies to their most exciting applications in biological laboratories.

Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.

متعدد خطوة الطباعة التصويرية متغير الطول لمحكم بصمام متعدد الأجهزة ميكروفلويديك

Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and directed within a layer of paper. Moreover, stacking multiple layers of patterned paper creates sophisticated three-dimensional microfluidic networks that can support the development of analytical and bioanalytical assays. Paper-based microfluidic devices are inexpensive, portable, easy to use, and require no external equipment to operate. As a result, they hold great promise as a platform for point-of-care diagnostics. In order to properly evaluate the utility and analytical performance of paper-based devices, suitable methods must be developed to ensure their manufacture is reproducible and at a scale that is appropriate for laboratory settings. In this manuscript, a method to fabricate a general device architecture that can be used for paper-based immunoassays is described. We use a form of additive manufacturing (multi-layer lamination) to prepare devices that comprise multiple layers of patterned paper and patterned adhesive. In addition to demonstrating the proper use of these three-dimensional paper-based microfluidic devices with an immunoassay for human chorionic gonadotropin (hCG), errors in the manufacturing process that may result in device failures are discussed. We expect this approach to manufacturing paper-based devices will find broad utility in the development of analytical applications designed specifically for limited-resource settings.

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

تصنيع أجهزة ميكروفلويديك ثلاثي الأبعاد على الورق ليمونواسايس

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

This paper reports a microfluidic device made of polydimethylsiloxane (PDMS) with an embedded polycarbonate (PC) thin film to study cell migration under combinations of chemical and oxygen gradients. Both chemical and oxygen gradients can greatly affect cell migration in vivo; however, due to technical limitations, very little research has been performed to investigate their effects in vitro. The device developed in this research takes advantage of a series of serpentine-shaped channels to generate the desired chemical gradients and exploits a spatially confined chemical reaction method for oxygen gradient generation. The directions of the chemical and oxygen gradients are perpendicular to each other to enable straightforward migration result interpretation. In order to efficiently generate the oxygen gradients with minimal chemical consumption, the embedded PC thin film is utilized as a gas diffusion barrier. The developed microfluidic device can be actuated by syringe pumps and placed into a conventional cell incubator during cell migration experiments to allow for setup simplification and optimized cell culture conditions. In cell experiments, we used the device to study migrations of adenocarcinomic human alveolar basal epithelial cells, A549, under combinations of chemokine (stromal cell-derived factor, SDF-1&#945;) and oxygen gradients. The experimental results show that the device can stably generate perpendicular chemokine and oxygen gradients and is compatible with cells. The migration study results indicate that oxygen gradients may play an essential role in guiding cell migration, and cellular behavior under combinations of gradients cannot be predicted from those under single gradients. The device provides a powerful and practical tool for researchers to study interactions between chemical and oxygen gradients in cell culture, which can promote better cell migration studies in more in vivo-like microenvironments.

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

ثنائي ميثيل بولي سيلوكسان البولي أجهزة ميكروفلويديك لخلية دراسات الهجرة تحت عمودي الكيميائية والتدرجات الأوكسجين

This paper reports a microfluidic device made of polydimethylsiloxane (PDMS) with an embedded polycarbonate (PC) thin film to study cell migration under ...

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices cannot be worn under everyday conditions. Therefore, textiles are commonly considered the best substrate to accommodate electronic devices in wearable use. In this paper, we describe how to selectively pattern organic electroactive materials on textiles from a solution in an easy and scalable manner. This versatile deposition technique enables the fabrication of wearable organic electronic devices on clothes.

In this paper, we present a protocol to selectively deposit organic materials on textiles, which allows for the direct integration of organic electronic devices with wearables. The fabricated devices can be fully integrated in textiles, respecting their mechanical appearance and enabling sensing capabilities.

وبسيطة وقابلة للتطوير أسلوب تلفيق لعضوي الأجهزة الإلكترونية بشأن المنسوجات

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices ...

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

In the last decade, microfluidic techniques have been applied to study small animals, including the nematode Caenorhabditis elegans, and have proved useful as a convenient live imaging platform providing capabilities for precise control of experimental conditions in real time. In this article, we demonstrate live imaging of individual worms employing WormSpa, a previously-published custom microfluidic device. In the device, multiple worms are individually confined to separate chambers, allowing multiplexed longitudinal surveillance of various biological processes. To illustrate the capability, we performed proof-of-principle experiments in which worms were infected in the device with pathogenic bacteria, and the dynamics of expression of immune response genes and egg laying were monitored continuously in individual animals. The simple design and operation of this device make it suitable for users with no previous experience with microfluidic-based experiments. We propose that this approach will be useful for many researchers interested in longitudinal observations of biological processes under well-defined conditions.

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

In this article, we demonstrate live imaging of individual worms employing a custom microfluidic device. In the device, multiple worms are individually confined to separate chambers, allowing multiplexed longitudinal surveillance of various biological processes.

منصة موائع جزيئية "التصوير الطولي" في ايليجانس كاينورهابديتيس

In the last decade, microfluidic techniques have been applied to study small animals, including the nematode Caenorhabditis elegans, and have proved ...