1. أخلاقيات الحيوان قبل تنفيذ ارتفاع الحرارة مدة طويلة على الفئران تأكد من أنه قد تم اعتماد هذا الإجراء من قبل رعاية الحيوان واللجنة المؤسسية الاستخدام. تمت الموافقة على جميع الإجراءات التي تمت مناقشتها في هذه الورقة وتظهر في شريط الفيديو المصاحب لجنة الأخلاقيات المؤسسية الحيوانية من المعهد الوطني للمناعة. 2. فهم ارتفاع الحرارة يتم تسليم كامل الجسم ارتفاع الحرارة (WBH) عن طريق وضع الحيوانات في أقفاص prewarmed التي يتم وضعها في حاضنة الحفاظ على 39،5 ° C. خلال توحيد البروتوكول، وزرع جراحيا جهاز استشعار درجة الحرارة في الفئران، ويتم رصد درجة حرارة الجسم في ظروف تجريبية مختلفة. لوحظ أنه عند وضع الحيوانات في البيئة الساخنة، درجة حرارتها تدريجيا من 37 ° C إلى تصل إلى 42 درجة مئوية خلال مدة 100 دقيقة (الشكل 1). اله مما أدى &quot;زيادة&quot; في درجة الحرارة فضول ودرجة حرارة الجسم الداخلية بالرصاص أعلى من درجة حرارة البيئة الخارجية. عندما تكون درجة حرارة الجسم تجاوزت 40 درجة مئوية، فإن الحيوان الذي تظهر علامات الإجهاد والجفاف مثل ترطيب حول الخطم. زيادة درجة حرارة الجسم أكثر من 40 درجة مئوية، سوف يؤدي إلى التبول والإجهاد لا رجعة فيه أو حتى إلى الوفاة من الحيوانات بسبب الإجهاد الحراري. لذلك من المهم لمنع ارتفاع درجة حرارة أثناء ارتفاع الحرارة. ويرتبط هذا ظاهرة ارتفاع درجة حرارة عالية مع فرط النشاط البدني من خلال التعرض الحيوان ارتفاع الحرارة. ارتفاع درجة الحرارة لوحظت من قبل مجموعات أخرى مماثلة جدا وارتبطت مع فرط النشاط البدني للحيوان على البيئة ليجري إدخال وجود ارتفاع درجات الحرارة 10. في بروتوكول لدينا، وثبتوا الفئران خلال التعرض ارتفاع الحرارة. كماهو موضح في الشكل 2، تجميد الحيوانات يمنع ارتفاع درجة الحرارة. وقد استخدمنا 8-12 أسابيع من العمر BALB / سي جيه الفئران لإثبات هذا البروتوكول. لا يقتصر البروتوكول إلى نوع معين / سلالة من الفئران. 3. التحضير للتجربة 3،1 حاضنة لا يمكن أن يؤديها في هذه التجربة حاضنة CO 2 طبيعي لما لها من التحكم في درجة الحرارة جيدة. قطع امدادات الغاز CO 2 و إزالة علبة المياه عقد الحاضنة 2 CO. في بعض نماذج من حاضنات فمن الممكن لإيقاف تشغيل وحدة تحكم CO 2. ترتيب رفوف الحاضنة بحيث يمكن وضع قفص الفئران مريح في الداخل. تعيين درجة حرارة الحاضنة في 39،5 ° C. 3،2 أقفاص عادة، يتم تنفيذ التجربة ارتفاع الحرارة في 4 الفئران في كل مرة. ويلزم اثنين أقفاص، بل علىيتم وضع البريد داخل الحاضنة ويتم وضع أخرى خارج في درجة حرارة الغرفة. يتم تجنب الفراش من الأقفاص، وبقع من الفراش الرطب تصبح مع مرور الوقت مما يؤدي إلى غير موحدة درجة الحرارة داخل القفص. يجب أن يكون خارج القفص الكريات الغذاء وزجاجة مياه. أنابيب تجميد 3،3 الفئران خلال أول 4 دورات من الفئران وارتفاع الحرارة في أنابيب مثقبة يجمد. ويمكن إجراء هذه الأنابيب من أنابيب تجميد &#39;الطرد المركزي&#39; 50 مل من الثقوب ثقب مم 3 في جميع أنحاء الجسم سوف تكون هناك حاجة لها وأربعة من الأنابيب من هذا القبيل. يمكن للأنبوب 50ML شل مريح BALB / سي جيه الفئران التي تكون أقدم من 8 أسابيع. قد تكون هناك حاجة أنابيب ذات أحجام مناسبة لبعض سلالة مختلفة من الفئران غير عادي أو الفئران التي هي صغيرة جدا أو مضى عليها أكثر من 50 أسبوعا. أنابيب تجميد المكان وقفص داخل الحاضنة الحفاظ على 39،5 ° C. انتظر على الأقل 2 ساعة بحيث القفص وأناأنابيب mmobilization بلوغ درجة حرارة الحاضنة. 4. أول دورة من ارتفاع الحرارة قد تواجه الجفاف الفئران خلال التعرض ارتفاع الحرارة. لذلك قبل البدء في التجربة وإدارة 0.5 مل الغشاء البريتونى المالحة الطبيعي أن كل الماوس. استخدام إبرة حقنة G 26 مع 1 مل. اتخاذ بسرعة القفص وضعها في الحاضنة، مكان واحد في كل الحيوانات أنابيب تجميد ووضع القفص في عودة الحاضنة. تسجيل الوقت. فمن الضروري للحفاظ على القفص في نفس المكان في كل مرة الحاضنة. بعد 90 دقيقة، واخراج القفص من الحاضنة. إطلاق سراح الحيوانات من أنابيب تجميد ووضعها في قفص في درجة حرارة طبيعية مع سهولة الوصول إلى الطعام والماء. وضع أنابيب تجميد فارغة مرة أخرى في الحاضنة. بعد 15 دقيقة، يتم تشغيل الدورة الثانية من ارتفاع الحرارة عن طريق تقديم 0.5 مل لكل الغشاء البريتونى المالحة الماوس. ويجمد بعد ذلك في 50 مل أنابيب مثقبة الشلل وضعها في الحاضنة. 5. دورات لاحقة ارتفاع الحرارة لأول 4 دورات، لا بد من شل الفئران خلال التعرض لارتفاع الحرارة ودورات الأولين، وتعطى حقن محلول ملحي قبل بدء فترة ارتفاع الحرارة. بعد الانتهاء من هذه الدورات من ارتفاع الحرارة تليها بقية والفئران والحصول على التأقلم لا تصبح مفرط خلال التعرض ارتفاع الحرارة. قد حقن داخل الصفاق من المياه المالحة لا يكون ضروريا إذا الفئران بسهولة اتخاذ الطعام والماء خلال فترة الراحة. 6. بعد كل شيء دورات المكتملة، والخطوة الأخيرة هي لفحص الحيوانات ينبغي ألا يكون هناك أي حالة وفاة خلال دورات ارتفاع الحرارة. كل حيوان لابد من مراقبته بحرص شديد في أولدورات قليلة. بحث بسرعة عن أي ترطيب حول الخطم. هذا العرض هو وجود توقيع من الجفاف. وينبغي على الفور الفئران مع مثل هذه الأعراض أن تعطى 0.5 مل IP المالحة العادية وبقية في درجة حرارة الغرفة. ومعظم الفئران يجمد لا يتطلب أي إضافية الرعاية ويتحمل بنجاح الضغط على ارتفاع الحرارة. بعد الانتهاء من الدورات المطلوبة، قد تبدو ضعيفة والحيوانات المجففة لكنها لن يتعافى سريعا في غضون ساعات قليلة. <img src="/files/ftp_upload/3801/3801fig1.jpg" alt="الشكل 1" /> الشكل 1. التغير في درجة حرارة الجسم الأساسية من الفئران عندما تم وضع القفص في حاضنة في 39،5 ° C. بعد 90 دقيقة تمت إزالة القفص من الحاضنة ووضعها عند 25 ° C. الدوائر الحمراء تشير إلى ارتفاع درجة حرارة عالية. <img src="/files/ftp_upload/3801/3801fig2.jpg" alt="الشكل 2" /> الشكل 2. التغير من جوهر الجسم temperatuلإعادة الفئران يجمد خلال دورات ارتفاع الحرارة. بعد شل حركة الحيوان، وارتفاع درجات الحرارة المسامير لا تتقيد عندما وضعت في قفص 39،5 ° C.

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ليس لدينا شيء في الكشف عنها.

<html> بقدر ما يعود إلى 5000 قبل الميلاد، وتعامل الأطباء المصريين مع الأورام الحرارة. اعترف الإغريق قيمة الحرارة في بعض العلاجات الطبية. معظم النصوص القديمة للقانون الساخنة الينابيع موسى ذكر (تكوين 36:24) لرفع درجة حرارة الجسم علاجيا. ال...

<table border="1" style=";text-align:right;direction:rtl"><tr><td></td><td></td><td></td><td></td></tr><tr><td> الثرمستور </td><td> RS مكونات </td><td> 528-8558 </td><td></td></tr><tr><td> CO 2 حاضنة </td><td> هيراوس </td><td> EK-B5060 CO2 </td><td></td></tr><tr><td> الفئران أقفاص </td><td> مواطن الصناعات المحدودة. </td><td></td><td> نوع B الطول 365X207X190 </td></tr><tr><td> أعلاف الحيوانات </td><td> Provimi الهند </td><td></td><td></td></tr><tr><td> زجاجة المياه لقفص </td><td> المورد المحلي </td><td></td><td> الزجاج زجاجة </td></tr><tr><td> لحام الحديد </td><td> Solderon، الهند </td><td></td><td></td></tr><tr><td> 50 مل أنابيب الطرد المركزي </td><td> BD العلوم البيولوجية </td><td> 352070 </td><td></td></tr><tr><td> مؤقت </td><td> المورد المحلي </td><td></td><td></td></tr><tr><td> 1 مل المحاقن مع إبرة 26G </td><td> BD العلوم البيولوجية </td><td> 309602 </td><td></td></tr><tr><td> ملحي </td><td> المورد المحلي </td><td></td><td></td></tr></table>

protocol for long duration whole body hyperthermia in mice

ارتفاع الحرارة هو مصطلح عام يستخدم لتحديد الزيادة في درجة حرارة الجسم فوق المعدل الطبيعي الأساسية. وكثيرا ما يستخدم لوصف الجسم الأساسية زيادة درجة الحرارة التي لوحظ أثناء الحمى. وقد برز ارتفاع الحرارة استخدام كمكمل علاجي كإجراء واعدة لانحسار الورم في مجال بيولوجيا السرطان. لهذا الغرض، والشرط الأهم هو أن تكون البروتوكولات التدفئة موثوقة وموحدة. قمنا بتطوير بروتوكول للارتفاع الحرارة (الجسم كله) في الفئران. في هذا البروتوكول، ويتعرض لدورات الحيوانات ارتفاع الحرارة لمدة 90 دقيقة تليها فترة راحة من 15 دقيقة. خلال هذه الفترة الفئران الوصول بسهولة إلى الغذاء والماء. ويمنع ارتفاع درجة حرارة الجسم العالية في الفئران خلال دورات القليلة الأولى من التعرض ارتفاع الحرارة شل حركة الحيوان. بالإضافة إلى ذلك، تدار المالحة الطبيعية في دورات القليلة الأولى للحد من آثار الجفاف. يمكن محاكاة هذا البروتوكول الحمى مثل الأوضاع في الفئران حتى 12-24 ساعة. لدينا8-12 أسابيع من العمر تستخدم BALB / سي جيه إناث الفئران للتدليل على البروتوكول.

Watch this Scientific Journal Video about Protocol for Long Duration Whole Body Hyperthermia in Mice at JoVE.com

Protocol for Long Duration Whole Body Hyperthermia in Mice

وتصف هذه الورقة بروتوكول لارتفاع الحرارة في الجسم كله الفئران التي يمكن أن تحفز مثل الحمى ظروف تصل إلى 12-24 ساعة.

بروتوكول لونغ ارتفاع الحرارة المدة الجسم الجامع في الفئران

Hyperthermia is a general term used to define the increase in core body temperature above normal. It is often used to describe the increased core body ...

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11.5K Views.  National Institute of Immunology. وتصف هذه الورقة بروتوكول لارتفاع الحرارة في الجسم كله الفئران التي يمكن أن تحفز مثل الحمى ظروف تصل إلى 12-24 ساعة.

Video: Protocol for Long Duration Whole Body Hyperthermia in Mice

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

Cancer therapies which are less toxic and invasive than their existing counterparts are highly desirable. The use of RF electric-fields that penetrate deep into the body, causing minimal toxicity, are currently being studied as a viable means of non-invasive cancer therapy. It is envisioned that the interactions of RF energy with internalized nanoparticles (NPs) can liberate heat which can then cause overheating (hyperthermia) of the cell, ultimately ending in cell necrosis.
In the case of non-biological systems, we present detailed protocols relating to quantifying the heat liberated by highly-concentrated NP colloids. For biological systems, in the case of in vitro experiments, we describe the techniques and conditions which must be adhered to in order to effectively expose cancer cells to RF energy without bulk media heating artifacts significantly obscuring the data. Finally, we give a detailed methodology for in vivo mouse models with ectopic hepatic cancer tumors.

We describe the protocols used to investigate the interactions of 13.56 MHz radiofrequency (RF) electric-fields with gold nanoparticle colloids in both non-biological and biological systems (in vitro/vivo). These interactions are being investigated for applications in cancer therapy.

بروتوكولات لتقييم الترددات الراديوية التفاعل مع جزيئات الذهب ونظم البيولوجية لغير الغازية ارتفاع الحرارة علاج السرطان

Cancer therapies  which are less toxic and invasive than their existing counterparts are highly  desirable. The use of RF electric-fields that penetrate ...

Parabiosis in Mice: A Detailed Protocol

Parabiosis is a surgical union of two organisms allowing sharing of the blood circulation. Attaching the skin of two animals promotes formation of microvasculature at the site of inflammation. Parabiotic partners share their circulating antigens and thus are free of adverse immune reaction. First described by Paul Bert in 18641, the parabiosis surgery was refined by Bunster and Meyer in 1933 to improve animal survival2. In the current protocol, two mice are surgically joined following a modification of the Bunster and Meyer technique. Animals are connected through the elbow and knee joints followed by attachment of the skin allowing firm support that prevents strain on the sutured skin. Herein, we describe in detail the parabiotic joining of a ubiquitous GFP expressing mouse to a wild type (WT) mouse. Two weeks after the procedure, the pair is separated and GFP positive cells can be detected by flow cytometric analysis in the blood circulation of the WT mouse. The blood chimerism allows one to examine the contribution of the circulating cells from one animal in the other.

Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.

تعايش التصاقي في الفئران: بروتوكول مفصل

Parabiosis is a surgical union of two organisms allowing sharing of the blood circulation. Attaching the skin of two animals promotes formation of ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study model. As the use of mouse models are often a vital step in preclinical drug discovery and drug development1-8, it is necessary to design a system to introduce drugs into mice in a uniform, reproducible manner. Ideally, the system should permit the collection of blood samples at regular intervals over a set time course. The ability to measure drug concentrations by mass-spectrometry, has allowed investigators to follow the changes in plasma drug levels over time in individual mice1, 9, 10. In this study, paclitaxel was introduced into transgenic mice as a continuous arterial infusion over three hours, while blood samples were simultaneously taken by retro-orbital bleeds at set time points. Carotid artery infusions are a potential alternative to jugular vein infusions, when factors such as mammary tumors or other obstructions make jugular infusions impractical. Using this technique, paclitaxel concentrations in plasma and tissue achieved similar levels as compared to jugular infusion. In this tutorial, we will demonstrate how to successfully catheterize the carotid artery by preparing an optimized catheter for the individual mouse model, then show how to insert and secure the catheter into the mouse carotid artery, thread the end of the catheter out through the back of the mouse&#8217;s neck, and hook the mouse to a pump to deliver a controlled rate of drug influx. Multiple low volume retro-orbital bleeds allow for analysis of plasma drug concentrations over time.

This method was developed with the goal of delivering a steady drug solution via the carotid artery, to assess the pharmacokinetics of novel drugs in mouse models.

الحقن الشريان السباتي لوأضافا الدوائية وتحليل Taxanes في الفئران

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Long-term Blood Pressure Measurement in Freely Moving Mice Using Telemetry

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. Indeed, research focusing on the discovery of new potential therapeutic targets using genetically altered mice requires a reliable, long-term assessment of the systemic arterial pressure variation. Currently, the gold standard for obtaining long-term measurements of blood pressure in ambulatory mice uses implantable radio-transmitters, which require&#160;artery cannulation. This technique eliminates the need for tethering, restraining, or anesthetizing the animals which introduce stress and artifacts during data sampling. However, arterial blood pressure monitoring in mice via catheterization can be rather challenging due to the small size of the arteries. Here we present a step-by-step guide to illustrate the crucial key passages for a successful subcutaneous implantation of radio-transmitters and carotid artery cannulation in mice. We also include examples of long-term blood pressure activity taken from freely moving mice after a period of post-surgery recovery. Following this procedure will allow reliable direct blood pressure recordings from multiple animals simultaneously.

The goal of this protocol is to assess systemic blood pressure in conscious freely moving mice using implantable radio-telemetry devices.

على المدى الطويل قياس ضغط الدم في الانتقال بحرية الفئران عن طريق القياس

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. ...

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Alterations to body composition (fat or lean mass), metabolic parameters such as whole-body oxygen consumption, energy expenditure, and substrate utilization, and behaviors such as food intake and physical activity can provide important information regarding the underlying mechanisms of disease. Given the importance of body composition and metabolism to the development of obesity and its subsequent sequelae, it is necessary to make accurate measures of these parameters in the pre-clinical research setting. Advances in technology over the past few decades have made it possible to derive these measures in rodent models in a non-invasive and longitudinal fashion. Consequently, these metabolic measures have proven useful when assessing the response of genetic manipulations (for example knockout or transgenic mice, viral knock-down or overexpression of genes), experimental drug/compound screening and dietary, behavioral or physical activity interventions. Herein, we describe the protocols used to measure body composition and metabolic parameters using an animal monitoring system in chow-fed and high fat diet-fed mice.

This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques.

تكوين الجسم والتحليل الأيضي حبس في الفئران بنك الاحتياطي الفيدرالي الدهون عالية

Alterations to body composition (fat or lean mass), metabolic parameters such as whole-body oxygen consumption, energy expenditure, and substrate ...

Protocol to Create Chronic Wounds in Diabetic Mice

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ~6.5M people and cost ~$40B/year in the US alone. Although a significant effort has been invested in understanding how chronic wounds develop in humans, fundamental questions remain unanswered. Recently, we developed a novel mouse model for diabetic chronic wounds that have many characteristics of human chronic wounds. Using db/db-/- mice, we can generate chronic wounds by inducing high levels of oxidative stress (OS) in the wound tissue immediately after wounding, using a one-time treatment with inhibitors specific to the antioxidant enzymes catalase and glutathione peroxidase. These wounds have high levels of OS, develop biofilm naturally, become fully chronic within 20 days after treatment and can remain open more for more than 60 days. This novel model has many features of diabetic chronic wounds in humans and therefore can contribute significantly to advancing fundamental understanding of how wounds become chronic. This is a major breakthrough because chronic wounds in humans cause significant pain and distress to patients and result in amputation if unresolved. Moreover, these wounds are very expensive and time-consuming to treat, and lead to significant loss of personal income to patients. Advancements in this field of study through the use of our chronic wound model can significantly improve health care for millions who suffer under this debilitating condition. In this protocol, we describe in great detail the procedure to cause acute wounds to become chronic, which has not been done before.

Chronic wounds are developed from acute wounds on a diabetic mouse model by inducing high levels of oxidative stress after a full-thickness cutaneous wound. The wound is treated with inhibitors for catalase and glutathione peroxidase, resulting in impaired healing and biofilm development by bacteria present in the skin microbiome.

بروتوكول لخلق الجروح المزمنة في الفئران السكري

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ...

Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a handheld metal detector to detect ingested metallic foreign bodies in children. We propose that by introducing handheld metal detector screening early in the triage process of children with high suspicion of metallic foreign body ingestion, the number of radiographs being ordered to localize the metallic foreign body can be reduced in this radio-sensitive population. The study protocol requires the screening of the participants for history of foreign body ingestion and exclusion of patients with respiratory distress or metallic implants. The patient changes to hospital gown and items that could contain metal like eyeglasses, earrings, pendants, and ornaments are removed. The patient is positioned in the center of the room away from other metallic interferences. The working status of the handheld metal detector is first confirmed by eliciting a positive audio-visual signal. Then the screening is done in an erect position with head in extension to expose the neck, from the level of the chin to the level of the hip joint, to cover the anatomical areas from neck to pelvis in a zig-zag manner both anteriorly and posteriorly. A positive audio-visual signal is carefully noted during the scanning for the presence of metallic foreign body. Relevant radiographs are ordered as per the area detected on the metal detector screening. The handheld metal detector was able to precisely identify all the coins among the ingested metallic foreign bodies in our study. The handheld metal detector could not consistently detect non-coin metallic foreign bodies. This protocol demonstrates the accuracy of handheld metal detector in the identification and localization of coins and coin like metallic foreign bodies.

Here, we present a protocol to use the handheld metal detector, to screen for the presence of ingested metallic foreign bodies in children who present to the pediatric emergency medicine department with a history of foreign body ingestion.

فرز يدوية للكشف عن المعادن لابتلاع جسم غريب المعدني في الأطفال

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a ...

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to expend energy has made it a potential target for novel therapies to ameliorate obesity and associated metabolic disorders in humans. Though this tissue has been well studied in small animals, BAT&#39;s thermogenic capacity in humans remains largely unknown due to the difficulties of measuring its volume, activity, and distribution. Identifying and quantifying active human BAT is commonly performed using 18F-Fluorodeoxyglucose (18F-FDG) positron emission tomography and computed tomography (PET/CT) scans following cold-exposure or pharmacological activation. Here we describe a detailed image-analysis approach to quantify total-body human BAT from 18F-FDG PET/CT scans using an open-source software. We demonstrate the drawing of user-specified regions of interest to identify metabolically active adipose tissue while avoiding common non-BAT tissues, to measure BAT volume and activity, and to further characterize its anatomical distribution. Although this rigorous approach is time-consuming, we believe it will ultimately provide a foundation to develop future automated BAT quantification algorithms.

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

Using free, open-source software, we have developed an analytical approach to quantify total and regional brown adipose tissue (BAT) volume and metabolic activity of BAT using 18F-FDG PET/CT.

الجسم كله والإقليمية الكمي لنشاط الإنسان براون الأنسجة الدهنية باستخدام 18وفدج PET/CT

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to ...

A Whole Body Dosimetry Protocol for Peptide-Receptor Radionuclide Therapy (PRRT): 2D Planar Image and Hybrid 2D+3D SPECT/CT Image Methods

Peptide-receptor-radionuclide-therapy (PPRT) is a targeted therapy that combines a short-range energy radionuclide with a substrate with high specificity for cancer cell receptors. After injection, the radiotracer is distributed throughout the entire body, with a higher uptake in tissues where targeted receptors are overexpressed. The use of beta/gamma radionuclide emitters enables therapy imaging (beta-emission) and post-therapy imaging (gamma-emission) to be performed at the same time. Post-treatment sequential images permit absorbed dose calculation based on local uptake and wash-in/wash-out kinetics. We implemented a hybrid method that combines information derived from both 2D and 3D images. Serial whole-body images and blood samples are acquired to estimate the absorbed dose to different organs at risk and to lesions disseminated throughout the body. A single 3D-SPECT/CT image, limited to the abdominal region, overcomes projection overlap on planar images of different structures such as the intestines and kidneys. The hybrid 2D+3D-SPECT/CT method combines the effective half-life information derived from 2D planar images with the local uptake distribution derived from 3D images. We implemented this methodology to estimate the absorbed dose for patients undergoing PRRT with 177Lu-PSMA-617. The methodology could, however, be implemented with other beta-gamma radiotracers. To date, 10 patients have been enrolled into the dosimetry study with 177Lu-PSMA-617 combined with drug protectors for kidneys and salivary glands (mannitol and glutamate tablets, respectively). The median ratio between kidney uptake at 24 h evaluated on planar images and 3D-SPECT/CT is 0.45 (range:0.32-1.23). The comparison between hybrid and full 3D approach has been tested on one patient, resulting in a 1.6% underestimation with respect to full 3D (2D: 0.829 mGy/MBq, hybrid: 0.315 mGy/MBq, 3D: 0.320 mGy/MBq). Treatment safety has been confirmed, with a mean absorbed dose of 0.73 mGy/MBq (range:0.26-1.07) for kidneys, 0.56 mGy/MBq (0.33-2.63) for the parotid glands and 0.63 mGy/MBq (0.23-1.20) for submandibular glands, values in accordance with previously published data.

A Whole Body Dosimetry Protocol for Peptide-Receptor Radionuclide Therapy PRRT: 2D Planar Image and Hybrid 2D+3D SPECT/CT Image Methods

This method estimates the absorbed dose of different structures for peptide-receptor-radionuclide-therapy (PRRT) with the possibility of avoiding organ overlap on 2D-projections. Serial whole-body planar images permit estimation of mean absorbed doses along the whole body, while the hybrid approach, combining planar images and 3D-SPECT/CT image, overcomes the limitations of structure overlapping.

بروتوكول قياس الخلايا الجسم كله لعلاج النويدات المشعة مستقبلات الببتيد (PRRT): 2D بلانار صورة والهجين 2D + 3D SPECT / CT طرق الصورة

Peptide-receptor-radionuclide-therapy (PPRT) is a targeted therapy that combines a short-range energy radionuclide with a substrate with high specificity ...

Assessing Whole-Body Lipid-Handling Capacity in Mice

Assessing lipid metabolism is a cornerstone of evaluating metabolic function, and it is considered essential for in vivo metabolism studies. Lipids are a class of many different molecules with many pathways involved in their synthesis and metabolism. A starting point for evaluating lipid hemostasis for nutrition and obesity research is needed. This paper describes three easy and accessible methods that require little expertise or practice to master, and that can be adapted by most labs to screen for lipid-metabolism abnormalities in mice. These methods are (1) measuring several fasting serum lipid molecules using commercial kits (2) assaying for dietary lipid-handling capability through an oral intralipid tolerance test, and (3) evaluating the response to a pharmaceutical compound, CL 316,243, in mice. Together, these methods will provide a high-level overview of lipid handling capability in mice.

This paper provides three easy and accessible assays for assessing lipid metabolism in mice.

تقييم قدرة معالجة الدهون في الجسم كله في الفئران

Assessing lipid metabolism is a cornerstone of evaluating metabolic function, and it is considered essential for in vivo metabolism studies. Lipids are a ...