Anthony A. James
Department of Molecular Biology and Biochemistry,
Department of Microbiology and Molecular Genetics,
Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics
University of California, Irvine (UCI)
Anthony A. James, PhD is Donald Bren Professor and Distinguished Professor of Microbiology & Molecular Genetics (School of Medicine) and Molecular Biology & Biochemistry (School of Biological Sciences) at the University of California, Irvine. He is a member of the National Academy of Sciences USA. His research group uses genetics as the basis for synthetic approaches to prevent transmission of mosquito-borne diseases. Contributions include the development of mosquito transgenesis procedures, engineered genes that interfere with malaria parasite or dengue virus development in mosquitoes, a population-suppression strain based on flightless female mosquitoes and gene-drive systems to spread beneficial genes quickly through mosquito populations.
Dr. James received his Bachelor of Science and PhD degrees at UCI. He went to Boston in 1979 for postdoctoral work (Harvard Medical School and Brandeis University) and joined the faculty at the Harvard School of Public Health in 1985. He returned to his alma mater in 1989, where he is today. Active and past support include multiple grants from the National Institutes of Health (USA), the Grand Challenges in Global Health initiative, the Bill and Melinda Gates Foundation, the Burroughs-Wellcome Fund, the John D. and Catherine T. MacArthur Foundation and the W.M. Keck Foundation. In addition to being elected to the National Academy of Sciences (USA) in 2006, he received numerous other awards including the Nan-Yao Su (2009) Award for Innovation and Creativity in Entomology from the Entomological Society of America, was a co-recipient of the Premio de Investgación Médica Dr. Jorge Rosenkranz (2008), a recipient of the Burroughs-Wellcome New Initiatives in Malaria Award (2000) and the Burroughs-Wellcome Scholar Award in Molecular Parasitology (1994). He was named a Fellow of the Royal Entomological Society of London (1992), Fellow of the American Association for the Advancement of Science (1994), Fellow of the Society, Entomological Society of America (2011) and Fellow of the American Society of Tropical Medicine and Hygiene (2012). In 2009, he was awarded the UCI Medal. He has published over 200 papers, reviews and policy documents and has provided guidance to 34 graduate students and postdoctoral fellows. He was a founding editor of the journal Insect Molecular Biology and has served on the editorial boards of PLoS Neglected Tropical Diseases, Experimental Parasitology and Entomological Research. He is a member of the American Society of Tropical Medicine and Hygiene, American Committee on Vector Entomology, American Association for the Advancement of Science, American Society of Parasitology, Royal Entomological Society, Entomological Society of America, Genetics Society of America and Society of Vector Ecology.
Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly.
Proceedings of the National Academy of Sciences of the United States of America Mar, 1998 | Pubmed ID: 9520437
Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti.
Proceedings of the National Academy of Sciences of the United States of America Mar, 1998 | Pubmed ID: 9520438
Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae.
Proceedings of the National Academy of Sciences of the United States of America Feb, 1999 | Pubmed ID: 9990055
Inhibition of luciferase expression in transgenic Aedes aegypti mosquitoes by Sindbis virus expression of antisense luciferase RNA.
Proceedings of the National Academy of Sciences of the United States of America , | Pubmed ID: 10557332
Controlling malaria transmission with genetically-engineered, Plasmodium-resistant mosquitoes: milestones in a model system.
Parassitologia Sep, 1999 | Pubmed ID: 10697903
Genetics of mosquito vector competence.
Microbiology and molecular biology reviews : MMBR Mar, 2000 | Pubmed ID: 10704476
Engineering blood meal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti.
Proceedings of the National Academy of Sciences of the United States of America Aug, 2000 | Pubmed ID: 10908672
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes.
Proceedings of the National Academy of Sciences of the United States of America Sep, 2000 | Pubmed ID: 11005862
What's that buzz? Mosquitoes and fruit flies commingle.
Parasitology today (Personal ed.) Dec, 2000 | Pubmed ID: 11121841
Development and applications of transgenesis in the yellow fever mosquito, Aedes aegypti.
Molecular and biochemical parasitology Apr, 2002 | Pubmed ID: 11985858
Germline transformants spreading out to many insect species.
Advances in genetics , 2002 | Pubmed ID: 12000097
Production of stage-specific Plasmodium falciparum cDNA libraries using subtractive hybridization.
Methods in molecular medicine , 2002 | Pubmed ID: 12125125
Engineering mosquito resistance to malaria parasites: the avian malaria model.
Insect biochemistry and molecular biology Oct, 2002 | Pubmed ID: 12225922
Malaria control with genetically manipulated insect vectors.
Science (New York, N.Y.) Oct, 2002 | Pubmed ID: 12364786
The major salivary gland antigens of Culex quinquefasciatus are D7-related proteins.
Insect biochemistry and molecular biology Jan, 2003 | Pubmed ID: 12459201
Engineering Plasmodium-refractory phenotypes in mosquitoes.
Trends in parasitology Sep, 2003 | Pubmed ID: 12957511
Blocking malaria parasite invasion of mosquito salivary glands.
The Journal of experimental biology Nov, 2003 | Pubmed ID: 14506217
Gene vector and transposable element behavior in mosquitoes.
The Journal of experimental biology Nov, 2003 | Pubmed ID: 14506218
High efficiency, site-specific excision of a marker gene by the phage P1 cre-loxP system in the yellow fever mosquito, Aedes aegypti.
Nucleic acids research Nov, 2003 | Pubmed ID: 14602940
The MB2 gene family of Plasmodium species has a unique combination of S1 and GTP-binding domains.
BMC bioinformatics Jun, 2004 | Pubmed ID: 15222903
Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti.
Insect biochemistry and molecular biology Jul, 2004 | Pubmed ID: 15242701
Formation and loss of large, unstable tandem arrays of the piggyBac transposable element in the yellow fever mosquito, Aedes aegypti.
Transgenic research Oct, 2004 | Pubmed ID: 15587266
Microarray analysis for identification of Plasmodium-refractoriness candidate genes in mosquitoes.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada Dec, 2004 | Pubmed ID: 15644964
Gene drive systems in mosquitoes: rules of the road.
Trends in parasitology Feb, 2005 | Pubmed ID: 15664528
Nanos (nos) genes of the vector mosquitoes, Anopheles gambiae, Anopheles stephensi and Aedes aegypti.
Insect biochemistry and molecular biology Jul, 2005 | Pubmed ID: 15894194
Characterization of the c-type lysozyme gene family in Anopheles gambiae.
Gene Nov, 2005 | Pubmed ID: 16137842
The novel Plasmodium gallinaceum sporozoite protein, Pg93, is preferentially expressed in the nucleus of oocyst sporozoites.
The American journal of tropical medicine and hygiene Sep, 2005 | Pubmed ID: 16172495
Bridging the gaps in vector biology. Workshop on the Molecular and Population Biology of Mosquitoes and other Disease Vectors.
EMBO reports Mar, 2006 | Pubmed ID: 16485025
Structure and expression of the lipophorin-encoding gene of the malaria vector, Anopheles gambiae.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology May, 2006 | Pubmed ID: 16524752
Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti.
Proceedings of the National Academy of Sciences of the United States of America Mar, 2006 | Pubmed ID: 16537508
A ubiquitous Plasmodium protein displays a unique surface labeling pattern in sporozoites.
Molecular and biochemical parasitology Aug, 2006 | Pubmed ID: 16697061
Expression of functional recombinant mosquito salivary apyrase: a potential therapeutic platelet aggregation inhibitor.
Platelets May, 2006 | Pubmed ID: 16702045
angaGEDUCI: Anopheles gambiae gene expression database with integrated comparative algorithms for identifying conserved DNA motifs in promoter sequences.
BMC genomics , 2006 | Pubmed ID: 16707020
Functional characterization of the promoter of the vitellogenin gene, AsVg1, of the malaria vector, Anopheles stephensi.
Insect biochemistry and molecular biology Sep, 2006 | Pubmed ID: 16935218
Biochemical mechanisms leading to tryptophan 2,3-dioxygenase activation.
Archives of insect biochemistry and physiology Feb, 2007 | Pubmed ID: 17212352
nanos gene control DNA mediates developmentally regulated transposition in the yellow fever mosquito Aedes aegypti.
Proceedings of the National Academy of Sciences of the United States of America Jun, 2007 | Pubmed ID: 17548819
Genetic control of malaria parasite transmission: threshold levels for infection in an avian model system.
The American journal of tropical medicine and hygiene Jun, 2007 | Pubmed ID: 17556613
The Anopheles gambiae vitellogenin gene (VGT2) promoter directs persistent accumulation of a reporter gene product in transgenic Anopheles stephensi following multiple bloodmeals.
The American journal of tropical medicine and hygiene Jun, 2007 | Pubmed ID: 17556621
Molecular genetic manipulation of vector mosquitoes.
Cell host & microbe Nov, 2008 | Pubmed ID: 18996342
Gene expression studies in mosquitoes.
Advances in genetics , 2008 | Pubmed ID: 19161831
Comparative genomics allows the discovery of cis-regulatory elements in mosquitoes.
Proceedings of the National Academy of Sciences of the United States of America Mar, 2009 | Pubmed ID: 19211788
From Tucson to genomics and transgenics: the vector biology network and the emergence of modern vector biology.
PLoS neglected tropical diseases , 2009 | Pubmed ID: 19333394
A transcriptome analysis of the Aedes aegypti vitellogenic fat body.
Journal of insect science (Online) , 2006 | Pubmed ID: 19537968
Stability and loss of a virus resistance phenotype over time in transgenic mosquitoes harbouring an antiviral effector gene.
Insect molecular biology , | Pubmed ID: 19754743
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals.
Malaria journal , 2009 | Pubmed ID: 19852802
Comparative fitness assessment of Anopheles stephensi transgenic lines receptive to site-specific integration.
Insect molecular biology Apr, 2010 | Pubmed ID: 20113372
Female-specific flightless phenotype for mosquito control.
Proceedings of the National Academy of Sciences of the United States of America Mar, 2010 | Pubmed ID: 20176967
Reframing critical needs in vector biology and management of vector-borne disease.
PLoS neglected tropical diseases , 2010 | Pubmed ID: 20186276
Towards a framework for community engagement in global health research.
Trends in parasitology Jun, 2010 | Pubmed ID: 20299285
Proteomics reveals novel components of the Anopheles gambiae eggshell.
Journal of insect physiology Oct, 2010 | Pubmed ID: 20433845
Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time.
Malaria journal , 2010 | Pubmed ID: 20470395
Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti.
Insect molecular biology , | Pubmed ID: 20738425
Dengue Fever in mainland China.
The American journal of tropical medicine and hygiene Sep, 2010 | Pubmed ID: 20810836
aeGEPUCI: a database of gene expression in the dengue vector mosquito, Aedes aegypti.
BMC research notes , 2010 | Pubmed ID: 20920356
Genome-wide patterns of gene expression during aging in the African malaria vector Anopheles gambiae.
PloS one , 2010 | Pubmed ID: 20967211
Genetics-based field studies prioritize safety.
Science (New York, N.Y.) Jan, 2011 | Pubmed ID: 21273469
RNA-seq analyses of blood-induced changes in gene expression in the mosquito vector species, Aedes aegypti.
BMC genomics , 2011 | Pubmed ID: 21276245
Genetic elimination of dengue vector mosquitoes.
Proceedings of the National Academy of Sciences of the United States of America Mar, 2011 | Pubmed ID: 21383140
A role for endosomal proteins in alphavirus dissemination in mosquitoes.
Insect molecular biology Aug, 2011 | Pubmed ID: 21496127
Engineered resistance to Plasmodium falciparum development in transgenic Anopheles stephensi.
PLoS pathogens Apr, 2011 | Pubmed ID: 21533066
Safety of genetically modified mosquitoes.
JAMA : the journal of the American Medical Association May, 2011 | Pubmed ID: 21610238
Why RIDL is not SIT.
Trends in parasitology Aug, 2011 | Pubmed ID: 21659002
Comparison of transgene expression in Aedes aegypti generated by mariner Mos1 transposition and ΦC31 site-directed recombination.
Insect molecular biology , | Pubmed ID: 21699593
Ecology. Mosquito trials.
Science (New York, N.Y.) Nov, 2011 | Pubmed ID: 22076370
Strain Variation in the Transcriptome of the Dengue Fever Vector, Aedes aegypti.
G3 (Bethesda, Md.) Jan, 2012 | Pubmed ID: 22384387
Transgenic Anopheles stephensi coexpressing single-chain antibodies resist Plasmodium falciparum development.
Proceedings of the National Academy of Sciences of the United States of America Jun, 2012 | Pubmed ID: 22689959
Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection.
PloS one , 2012 | Pubmed ID: 23209765
Field cage studies and progressive evaluation of genetically-engineered mosquitoes.
PLoS neglected tropical diseases , 2013 | Pubmed ID: 23350003
Strongyloidiasis: an emerging infectious disease in China.
The American journal of tropical medicine and hygiene Mar, 2013 | Pubmed ID: 23468357
Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.
Proceedings of the National Academy of Sciences of the United States of America Apr, 2013 | Pubmed ID: 23584017
Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi.
Malaria journal , 2013 | Pubmed ID: 23622561
orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET.
Nature Jun, 2013 | Pubmed ID: 23719379
miRNA genes of an invasive vector mosquito, Aedes albopictus.
PloS one , 2013 | Pubmed ID: 23840875
The invasive mosquito species Aedes albopictus: current knowledge and future perspectives.
Trends in parasitology Sep, 2013 | Pubmed ID: 23916878
Molecular and functional characterization of odorant-binding protein genes in an invasive vector mosquito, Aedes albopictus.
PloS one , 2013 | Pubmed ID: 23935894
Gene expression-based biomarkers for Anopheles gambiae age grading.
PloS one , 2013 | Pubmed ID: 23936017
Probing functional polymorphisms in the dengue vector, Aedes aegypti.
BMC genomics , 2013 | Pubmed ID: 24168143
Genome-block expression-assisted association studies discover malaria resistance genes in Anopheles gambiae.
Proceedings of the National Academy of Sciences of the United States of America Dec, 2013 | Pubmed ID: 24297936
Expression and accumulation of the two-domain odorant-binding protein AaegOBP45 in the ovaries of blood-fed Aedes aegypti.
Parasites & vectors , 2013 | Pubmed ID: 24367904
A regulatory structure for working with genetically modified mosquitoes: lessons from Mexico.
PLoS neglected tropical diseases Mar, 2014 | Pubmed ID: 24626164
Criteria for identifying and evaluating candidate sites for open-field trials of genetically engineered mosquitoes.
Vector borne and zoonotic diseases (Larchmont, N.Y.) Apr, 2014 | Pubmed ID: 24689963
Integrated proteomic and transcriptomic analysis of the Aedes aegypti eggshell.
BMC developmental biology , 2014 | Pubmed ID: 24707823
Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti.
Proceedings of the National Academy of Sciences of the United States of America May, 2014 | Pubmed ID: 24778255
Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.
PLoS neglected tropical diseases May, 2014 | Pubmed ID: 24810399
Transcriptome sequencing and developmental regulation of gene expression in Anopheles aquasalis.
PLoS neglected tropical diseases Jul, 2014 | Pubmed ID: 25033462
Transcription activator-like effector nuclease (TALEN)-mediated female-specific sterility in the silkworm, Bombyx mori.
Insect molecular biology Dec, 2014 | Pubmed ID: 25125145
Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi.
Genome biology Sep, 2014 | Pubmed ID: 25244985
Site-specific, TALENs-mediated transformation of Bombyx mori.
Insect biochemistry and molecular biology Oct, 2014 | Pubmed ID: 25460511
Maternal germline-specific genes in the Asian malaria mosquito Anopheles stephensi: characterization and application for disease control.
G3 (Bethesda, Md.) Feb, 2015 | Pubmed ID: 25480960
Molecular epidemiology of Plasmodium vivax and Plasmodium falciparum malaria among Duffy-positive and Duffy-negative populations in Ethiopia.
Malaria journal Feb, 2015 | Pubmed ID: 25884875
Protein phosphorylation during Plasmodium berghei gametogenesis.
Experimental parasitology Sep, 2015 | Pubmed ID: 26008612
MiR-2 family targets awd and fng to regulate wing morphogenesis in Bombyx mori.
RNA biology , 2015 | Pubmed ID: 26037405
Genome sequence of the Asian Tiger mosquito, Aedes albopictus, reveals insights into its biology, genetics, and evolution.
Proceedings of the National Academy of Sciences of the United States of America Nov, 2015 | Pubmed ID: 26483478
Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi.
Proceedings of the National Academy of Sciences of the United States of America Dec, 2015 | Pubmed ID: 26598698
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands.
Malaria journal Mar, 2016 | Pubmed ID: 26964736
CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in the global pest, diamondback moth (Plutella xylostella).
Insect biochemistry and molecular biology 08, 2016 | Pubmed ID: 27318252
Functional analysis of Orco and odorant receptors in odor recognition in Aedes albopictus.
Parasites & vectors 06, 2016 | Pubmed ID: 27350348
Sexually dimorphic traits in the silkworm, Bombyx mori, are regulated by doublesex.
Insect biochemistry and molecular biology 01, 2017 | Pubmed ID: 27867075
Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.
PLoS genetics 01, 2017 | Pubmed ID: 28103247
Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus.
Journal of virology 04, 2017 | Pubmed ID: 28122981
Lys48 ubiquitination during the intraerythrocytic cycle of the rodent malaria parasite, Plasmodium chabaudi.
PloS one , 2017 | Pubmed ID: 28604779
nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.
Insect biochemistry and molecular biology 08, 2017 | Pubmed ID: 28676355
Rules of the road for insect gene drive research and testing.
Nature biotechnology 08, 2017 | Pubmed ID: 28787415
Population modification of Anopheline species to control malaria transmission.
Pathogens and global health 12, 2017 | Pubmed ID: 29385893
Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.
PLoS genetics 02, 2018 | Pubmed ID: 29474354
The redox-sensing gene affects intestinal homeostasis, insecticide resistance, and Zika virus susceptibility in the mosquito .
The Journal of biological chemistry 06, 2018 | Pubmed ID: 29685890
Silkworm genetic sexing through W chromosome-linked, targeted gene integration.
Proceedings of the National Academy of Sciences of the United States of America 08, 2018 | Pubmed ID: 30104361
Fall webworm genomes yield insights into rapid adaptation of invasive species.
Nature ecology & evolution 01, 2019 | Pubmed ID: 30532047
Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans.
Insect biochemistry and molecular biology 01, 2020 | Pubmed ID: 31541694
Experimental population modification of the malaria vector mosquito, Anopheles stephensi.
PLoS genetics 12, 2019 | Pubmed ID: 31856182
Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species .
BioTechniques 04, 2020 | Pubmed ID: 32040336
Cas9-Mediated Gene-Editing in the Malaria Mosquito by ReMOT Control.
G3 (Bethesda, Md.) 04, 2020 | Pubmed ID: 32122959
Next-generation gene drive for population modification of the malaria vector mosquito, .
Proceedings of the National Academy of Sciences of the United States of America 09, 2020 | Pubmed ID: 32839345
The [] Gene Family Modulates Dengue Virus Infection in .
International journal of molecular sciences Oct, 2020 | Pubmed ID: 33053895
Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi.
Nature communications 11, 2020 | Pubmed ID: 33144570
Application of the Relationship-Based Model to Engagement for Field Trials of Genetically Engineered Malaria Vectors.
The American journal of tropical medicine and hygiene Dec, 2020 | Pubmed ID: 33350374
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes
