JoVE Logo
Faculty Resource Center

Sign In

Injection of dsRNA into Female A. aegypti Mosquitos

DOI :

10.3791/215-v

July 4th, 2007

July 4th, 2007

11,998 Views

1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics, University of California, Irvine (UCI)

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Tags

DsRNA Injection

-- Views

Related Videos

article

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

article

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

article

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

article

Intraperitoneal Injection into Adult Zebrafish

article

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

article

RNAi Interference by dsRNA Injection into Drosophila Embryos

article

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

article

Measuring Peptide Translocation into Large Unilamellar Vesicles

article

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

article

RNAi Trigger Delivery into Anopheles gambiae Pupae

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved