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Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes Video (Video) | JoVE

DOI :

10.3791/219-v

July 4th, 2007

July 4th, 2007

14,797 Views

1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics, University of California, Irvine (UCI)

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.

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