Albert Jeltsch

DNA methylation analysis of chromosome 21 gene promoters at single base pair and single allele resolution.

Site-directed mutagenesis by polymerase chain reaction.

Molecular enzymology of the catalytic domains of the Dnmt3a and Dnmt3b DNA methyltransferases.

Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.

A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleases.

The Escherichia coli dam DNA methyltransferase modifies DNA in a highly processive reaction.

Specificity of DNA triple helix formation analyzed by a FRET assay.

Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA.

Probing the DNA interface of the EcoRV DNA-(adenine-N6)-methyltransferase by site-directed mutagenesis, fluorescence spectroscopy, and UV cross-linking.

High-throughput purification of polyHis-tagged recombinant fusion proteins.

Detection and analysis of enzymatic DNA methylation of oligonucleotide substrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.

Methylation sensitivity of restriction enzymes interacting with GATC sites.

Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzyme.

The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.

Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII.

Maintenance of species identity and controlling speciation of bacteria: a new function for restriction/modification systems?

Chromatin targeting of de novo DNA methyltransferases by the PWWP domain.

Stopped-flow and mutational analysis of base flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase.

The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites.

Anomalous mobility of polymerase chain reaction products after bisulfite treatment of DNA.

Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapy.

Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L.

Profound flanking sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenome.

Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase.

Avidin plate assay system for enzymatic characterization of a histone lysine methyltransferase.

De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases.

Developing a programmed restriction endonuclease for highly specific DNA cleavage.

Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase.

Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferase.

Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase.

Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L.

Two substrates are better than one: dual specificities for Dnmt2 methyltransferases.

German human methylome project started.

Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes.

Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group.

Application of DNA methyltransferases in targeted DNA methylation.

Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle.

Deoxyribonucleic acid methyl transferases 3a and 3b associate with the nuclear orphan receptor COUP-TFI during gene activation.

Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.

Phosphorylation of serine-515 activates the Mammalian maintenance methyltransferase Dnmt1.

On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme.

The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition site.

Continuous enzymatic assay for histone lysine methyltransferases.

Mapping of protein-protein interaction sites by the 'absence of interference' approach.

Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays.

Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay.

Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysis.

Cyclical DNA methylation of a transcriptionally active promoter.

Persistent downregulation of the pancarcinoma-associated epithelial cell adhesion molecule via active intranuclear methylation.

Protein lysine methyltransferase G9a acts on non-histone targets.

Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism.

Putting the pieces together: histone H2B ubiquitylation directly stimulates histone H3K79 methylation.

Reading and writing DNA methylation.

Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L.

DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones.

Impact of 7,8-dihydro-8-oxoguanine on methylation of the CpG site by Dnmt3a.

Specificity of protein lysine methyltransferases and methods for detection of lysine methylation of non-histone proteins.

The ankyrin repeat domain of Huntingtin interacting protein 14 contains a surface aromatic cage, a potential site for methyl-lysine binding.

On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.

Transition from EcoDam to T4Dam DNA recognition mechanism without loss of activity and specificity.

A continuous protein methyltransferase (G9a) assay for enzyme activity measurement and inhibitor screening.

Changing the DNA recognition specificity of the EcoDam DNA-(adenine-N6)-methyltransferase by directed evolution.

New clustering module in BDPC bisulfite sequencing data presentation and compilation web application for DNA methylation analyses.

Non-imprinted allele-specific DNA methylation on human autosomes.

Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail.

BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences.

Molecular biology. Phylogeny of methylomes.

The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation.

Silencing of gene expression by targeted DNA methylation: concepts and approaches.

Mechanistic insights on the inhibition of c5 DNA methyltransferases by zebularine.

Detailed specificity analysis of antibodies binding to modified histone tails with peptide arrays.

Molecular epigenetics: connecting human biology and disease with little marks.

Structure and function of mammalian DNA methyltransferases.

Specificity analysis-based identification of new methylation targets of the SET7/9 protein lysine methyltransferase.

Approaches to enzyme and substrate design of the murine Dnmt3a DNA methyltransferase.

The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9.

Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue.

The inhibition of the mammalian DNA methyltransferase 3a (Dnmt3a) by dietary black tea and coffee polyphenols.

Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L.

Histone tails regulate DNA methylation by allosterically activating de novo methyltransferase.

C5-DNA methyltransferase inhibitors: from screening to effects on zebrafish embryo development.

Application of Celluspots peptide arrays for the analysis of the binding specificity of epigenetic reading domains to modified histone tails.

DNA methyltransferase assays.

The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner.

Function and disruption of DNA methyltransferase 3a cooperative DNA binding and nucleoprotein filament formation.

Using the fluorescence decay of 2-aminopurine to investigate conformational change in the recognition sequence of the EcoRV DNA-(adenine-N6)-methyltransferase on enzyme binding.

Burning off DNA methylation: new evidence for oxygen-dependent DNA demethylation.

Epigenetics Europe conference. Munich, Germany, 8-9 September 2011.

On the evolutionary origin of eukaryotic DNA methyltransferases and Dnmt2.

Rapid synthesis of new DNMT inhibitors derivatives of procainamide.

A fluorescence-based supramolecular tandem assay for monitoring lysine methyltransferase activity in homogeneous solution.

The SET8 H4K20 protein lysine methyltransferase has a long recognition sequence covering seven amino acid residues.

Mapping the tRNA binding site on the surface of human DNMT2 methyltransferase.

Specificity of Dnmt1 for methylation of hemimethylated CpG sites resides in its catalytic domain.

DNA interaction of the CcrM DNA methyltransferase: a mutational and modeling study.

Mechanistic details of the DNA recognition by the Dnmt1 DNA methyltransferase.

Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase.

DNA methyltransferase inhibitors in cancer: a chemical and therapeutic patent overview and selected clinical studies.

Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling.

Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity.

Identification of novel inhibitors of DNA methylation by screening of a chemical library.

Oxygen, epigenetic signaling, and the evolution of early life.

Multimerization of the dnmt3a DNA methyltransferase and its functional implications.

Non-radioactive protein lysine methyltransferase microplate assay based on reading domains.

Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA).

The Tudor domain of the PHD finger protein 1 is a dual reader of lysine trimethylation at lysine 36 of histone H3 and lysine 27 of histone variant H3t.

Synthesis and evaluation of analogues of N-phthaloyl-l-tryptophan (RG108) as inhibitors of DNA methyltransferase 1.

Genomic imprinting--the struggle of the genders at the molecular level.

The UHRF1 protein stimulates the activity and specificity of the maintenance DNA methyltransferase DNMT1 by an allosteric mechanism.

Substrate specificity analysis and novel substrates of the protein lysine methyltransferase NSD1.

Targeted methylation of the epithelial cell adhesion molecule (EpCAM) promoter to silence its expression in ovarian cancer cells.

Targeted mutagenesis results in an activation of DNA methyltransferase 1 and confirms an autoinhibitory role of its RFTS domain.

Design, synthesis and biological evaluation of 4-amino-N- (4-aminophenyl)benzamide analogues of quinoline-based SGI-1027 as inhibitors of DNA methylation.

The application of next generation sequencing in DNA methylation analysis.

The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu.

Label-free measurement of histone lysine methyltransferases activity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

New concepts in DNA methylation.

Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a.

Role of somatic cancer mutations in human protein lysine methyltransferases.

Cooperative DNA Binding and Protein/DNA Fiber Formation Increases the Activity of the Dnmt3a DNA Methyltransferase.

Application of histone modification-specific interaction domains as an alternative to antibodies.