Michael Karas

Carbon Source-dependent assembly of the Snf1p kinase complex in Candida albicans.

Comparative proteomic analysis of the rat spinal cord in inflammatory and neuropathic pain models.

Mass spectrometric sequencing of individual peptides from combinatorial libraries via specific generation of chain-terminated sequences.

Identification of anhydroecgonine methyl ester N-oxide, a new metabolite of anhydroecgonine methyl ester, using electrospray mass spectrometry.

Formation and fate of ion pairs during MALDI analysis: anion adduct generation as an indicative tool to determine ionization processes.

Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MS(n) (part 1: methodology).

Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MS(n) (part 2: application to isomeric mixtures).

Ion formation in MALDI: the cluster ionization mechanism.

Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr.

Effect of different solution flow rates on analyte ion signals in nano-ESI MS, or: when does ESI turn into nano-ESI?

The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells.

Degradation of ginsenosides in humans after oral administration.

Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation.

Determination of hyperforin in mouse brain by high-performance liquid chromatography/tandem mass spectrometry.

Multiple signaling pathways are involved in the regulation of IGF-I receptor inhibition of PTEN-enhanced apoptosis.

Calibration of ion effective temperatures achieved by resonant activation in a quadrupole ion trap.

PTEN inhibits cell proliferation and induces apoptosis by downregulating cell surface IGF-IR expression in prostate cancer cells.

Effects of organ-specific loss of insulin-like growth factor-I production on murine hematopoiesis.

TDAG51 mediates the effects of insulin-like growth factor I (IGF-I) on cell survival.

14-3-3sigma mediation of cell cycle progression is p53-independent in response to insulin-like growth factor-I receptor activation.

Physicochemical characterisation of cationic polybutylcyanoacrylate-nanoparticles by fluorescence correlation spectroscopy.

Mass spectrometric mapping of the enzymes involved in the phenol degradation of an indigenous soil pseudomonad.

CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA class Ia/Ib-associated renal carcinoma antigens with ubiquitous or restricted tissue expression.

Internal energy build-up in matrix-assisted laser desorption/ionization.

Insulin-like growth factor-I stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes.

Processing of the 24 kDa subunit mitochondrial import signal is not required for assembly of functional complex I in Yarrowia lipolytica.

Hyperforin modifies neuronal membrane properties in vivo.

Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification.

Chronic administration of statins alters multiple gene expression patterns in mouse cerebral cortex.

Membrane proteome analysis of the green-sulfur bacterium Chlorobium tepidum.

Detection and characterisation of catechol 2,3-dioxygenase in an indigenous soil pseudomonad by MALDI-TOF MS using a column separation.

Lipid rafts associate with intracellular B cell receptors and exhibit a B cell stage-specific protein composition.

Use of two-dimensional gel electrophoresis in predictive toxicology: identification of potential early protein biomarkers in chemically induced hepatocarcinogenesis.

A four-subunit cytochrome bc(1) complex complements the respiratory chain of Thermus thermophilus.

Molecular weights of poly(butyl cyanoacrylate) nanoparticles determined by mass spectrometry and size exclusion chromatography.

Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by Nano-LC MALDI MS/MS.

LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis.

Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.

Proteomic analysis of chlorosome-depleted membranes of the green sulfur bacterium Chlorobium tepidum.

Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry.

High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of cytoplasmatic protein complexes from Chlorobium tepidum.

Functional characterization of BbCRASP-2, a distinct outer membrane protein of Borrelia burgdorferi that binds host complement regulators factor H and FHL-1.

Synaptic vesicle proteins under conditions of rest and activation: analysis by 2-D difference gel electrophoresis.

Mass spectrometric analysis of glycine receptor-associated gephyrin splice variants.

Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus.

Analysis of the synaptic vesicle proteome using three gel-based protein separation techniques.

Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy.

An alternative strategy for the membrane proteome analysis of the green sulfur bacterium Chlorobium tepidum using blue native PAGE and 2-D PAGE on purified membranes.

High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes.

Prevalidation of potential protein biomarkers in toxicology using iTRAQ reagent technology.

A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding.

Identification of two proteins associated with mammalian ATP synthase.

Investigation of sample-purification procedures for MALDI-based proteomic studies.

Scaffold hopping by "fuzzy" pharmacophores and its application to RNA targets.

Components of the translational machinery are associated with juvenile glycine receptors and are redistributed to the cytoskeleton upon aging and synaptic activity.

The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric.

Metabolism of boswellic acids in vitro and in vivo.

Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.

Using fluorescence dyes as a tool for analyzing the MALDI process.

Detection and relative quantification of siRNA double strands by MALDI mass spectrometry.

Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells.

Comparison of the membrane subproteomes during growth of a new pseudomonas strain on lysogeny broth medium, glucose, and phenol.

4-Chloro-alpha-cyanocinnamic acid is an advanced, rationally designed MALDI matrix.

Elastase digests: new ammunition for shotgun membrane proteomics.

The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.

The new matrix 4-chloro-alpha-cyanocinnamic acid allows the detection of phosphatidylethanolamine chloramines by MALDI-TOF mass spectrometry.

Sequencing of single and double stranded RNA oligonucleotides by acid hydrolysis and MALDI mass spectrometry.

Comparison between vacuum sublimed matrices and conventional dried droplet preparation in MALDI-TOF mass spectrometry.

Comparison between the matrices alpha-cyano-4-hydroxycinnamic acid and 4-chloro-alpha-cyanocinnamic acid for trypsin, chymotrypsin, and pepsin digestions by MALDI-TOF mass spectrometry.

Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteasomal degradation.

Identification of human cathepsin G as a functional target of boswellic acids from the anti-inflammatory remedy frankincense.

Quantitative site-specific analysis of protein glycosylation by LC-MS using different glycopeptide-enrichment strategies.

The benefit of combining nLC-MALDI-Orbitrap MS data with nLC-MALDI-TOF/TOF data for proteomic analyses employing elastase.

Metabotropic glutamate receptor 4 interacts with microtubule-associated protein 1B.

Rapid simultaneous quantitative determination of different small pharmaceutical drugs using a conventional matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system.

Highly specific capture and direct MALDI MS analysis of phosphopeptides by zirconium phosphonate on self-assembled monolayers.

Membrane protein analysis using an improved peptic in-solution digestion protocol.

The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.

Quantitative determination of acetylcholine and choline in microdialysis samples by MALDI-TOF MS.

Approaching the complexity of elastase-digested membrane proteomes using off-gel IEF/nLC-MALDI-MS/MS.

A novel polyacrylamide gel system for proteomic use offering controllable pore expansion by crosslinker cleavage.

Mass estimation of native proteins by blue native electrophoresis: principles and practical hints.

Assembly and oligomerization of human ATP synthase lacking mitochondrial subunits a and A6L.

Enhanced glyco-profiling by specific glycopeptide enrichment and complementary monolithic nano-LC (ZIC-HILIC/RP18e)/ESI-MS analysis.

Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods.

Peptide mass fingerprinting after less specific in-gel proteolysis using MALDI-LTQ-Orbitrap and 4-chloro-alpha-cyanocinnamic acid.

3-Aminoquinoline acting as matrix and derivatizing agent for MALDI MS analysis of oligosaccharides.

Analysis of boronic acids by nano liquid chromatography-direct electron ionization mass spectrometry.

Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass spectrometric study of lipids from hen egg yolk.

100% protein sequence coverage: a modern form of surrealism in proteomics.

Identification of novel human adipocyte secreted proteins by using SGBS cells.

Hypoxia and reoxygenation of primary human hepatocytes induce proteome changes of glucose metabolism, oxidative protection and peroxisomal function.

Labeling elastase digests with TMT: informational gain by identification of poorly detectable peptides with MALDI-TOF/TOF mass spectrometry.

The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA.

Complement factor H-related proteins CFHR2 and CFHR5 represent novel ligands for the infection-associated CRASP proteins of Borrelia burgdorferi.

Entianin, a novel subtilin-like lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with high antimicrobial activity.

Proteome profiling suggests a pro-inflammatory role for plasma cells through release of high-mobility group box 1 protein.

A high-definition native polyacrylamide gel electrophoresis system for the analysis of membrane complexes.

Structure elucidation and biosynthesis of lysine-rich cyclic peptides in Xenorhabdus nematophila.

Simple dual-spotting procedure enhances nLC-MALDI MS/MS analysis of digests with less specific enzymes.

A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains.

Nerve injury evoked loss of latexin expression in spinal cord neurons contributes to the development of neuropathic pain.

Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome.

The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor β-subunit.

Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

High-reactivity matrices increase the sensitivity of matrix enhanced secondary ion mass spectrometry.

Compelling evidence for Lucky Survivor and gas phase protonation: the unified MALDI analyte protonation mechanism.

Nano-liquid chromatography-direct electron ionization mass spectrometry: improving performance by a new ion source adapter.

Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416.

Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.

Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors.

Perfusion reversed-phase high-performance liquid chromatography for protein separation from detergent-containing solutions: an alternative to gel-based approaches.

Premature transcript termination, trans-splicing and DNA repair: a vicious path to cancer.

Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins.

Ion yields in UV-MALDI mass spectrometry as a function of excitation laser wavelength and optical and physico-chemical properties of classical and halogen-substituted MALDI matrixes.

Neutral loss fragmentation pattern based screening for arginine-rich natural products in Xenorhabdus and Photorhabdus.

Graphite supported preparation (GSP) of α-cyano-4-hydroxycinnamic acid (CHCA) for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for peptides and proteins.

Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.

Enzyme-cleavable tandem peptides for quantitative studies in MS-based proteomics.

Comparative analysis of barley leaf proteome as affected by drought stress.

Defining the core proteome of the chloroplast envelope membranes.

TEMPORARY REMOVAL: Adsorption of plasma proteins on uncoated PLGA nanoparticles.

Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk.

MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels.

Enhanced MALDI MS sensitivity by weak base additives and glycerol sample coating.

Sulfo-NHS-SS-biotin derivatization: a versatile tool for MALDI mass analysis of PTMs in lysine-rich proteins.

The proteome of the presynaptic active zone from mouse brain.

Analysis of 5-lipoxygenase phosphorylation on molecular level by MALDI-MS.

PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation.

Secretome analysis of Anabaena sp. PCC 7120 and the involvement of the TolC-homologue HgdD in protein secretion.

Self-built microdialysis probes with improved recoveries of ATP and neuropeptides.

Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery.

The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21.

Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination.