Miguel Alcalde

Modification of galactose oxidase to introduce glucose 6-oxidase activity.

Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.

Screening mutant libraries in Saccharomyces cerevisiae.

Colorimetric assays for screening laccases.

Preparing libraries in Saccharomyces cerevisiae.

Colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases.

Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3.

Effect of carbohydrate fatty acid esters on Streptococcus sobrinus and glucosyltransferase activity.

Use and improvement of microbial redox enzymes for environmental purposes.

Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports.

Screening mutant libraries of fungal laccases in the presence of organic solvents.

Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation.

Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299.

Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides.

Environmental biocatalysis: from remediation with enzymes to novel green processes.

Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.

Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases.

Transformation of polycyclic aromatic hydrocarbons by laccase is strongly enhanced by phenolic compounds present in soil.

In vitro evolution of a fungal laccase in high concentrations of organic cosolvents.

Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra.

Engineering and Applications of fungal laccases for organic synthesis.

Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide.

Laccases and their applications: a patent review.

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.

Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension.

Laboratory evolution of high-redox potential laccases.

Screening β-fructofuranosidases mutant libraries to enhance the transglycosylation rates of β-(2→6) fructooligosaccharides.

Directed evolution of fungal laccases.

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase.

Risk of recurrence of non-metastatic breast cancer in women under 40 years: a population-registry cancer study in a European country.

Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports.

Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes.

Development of chimeric laccases by directed evolution.

Covalent immobilization of cyclodextrin glucosyltransferase (CGTase) in activated silica and Sepharose.

Blood tolerant laccase by directed evolution.

Widening the pH activity profile of a fungal laccase by directed evolution.

Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production.

Functional expression of a blood tolerant laccase in Pichia pastoris.

New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass.

Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.

Directed evolution of unspecific peroxygenase from Agrocybe aegerita.

Bioelectrochemical oxidation of water.

Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae.

Self-powered wireless carbohydrate/oxygen sensitive biodevice based on radio signal transmission.

Laccase engineering: from rational design to directed evolution.

Engineering the ligninolytic enzyme consortium.

Tandem-yeast expression system for engineering and producing unspecific peroxygenase.

Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.

Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol.

Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution.