production-chemicals-klebsiella-pneumoniae-using-bamboo-hydrolysate

Production of Chemicals by <em>Klebsiella pneumoniae</em> Using Bamboo Hydrolysate as Feedstock

Bamboo is an important biomass, and bamboo hydrolysate is used by Klebsiella pneumoniae as a feedstock for chemical production. Here, bamboo powder was ...

Chinese Academy of Sciences

Pyruvate dehydrogenase-complex (AcoABCD) and pyruvate formate-lyase (PFL) are two pathways responsible for synthesis of acetyl-CoA from pyruvate (pyruvate acetyl-CoA switch). The two pathways were individually deleted in Klebsiella pneumoniae, and the role of the pyruvate acetyl-CoA switch in 1,3-propanediol production was investigated. Fermentation results showed that the two pathways were both active in the wild-type strain. Acetyl-CoA formation between the two pathways was equal in the wild-type strain. The pflB mutant produced high level of lactic acid, and deletion of ldhA eliminated lactic acid synthesis. The conversion ratio of glycerol to 1,3-propanediol in the pflB-ldhA mutant reached 0.541 g/g, which was 9.4 % higher than that of the wild-type strain. However, the productivity of 1,3-propanediol was decreased in the pflB-ldhA mutant. In contrast, the productivity of 1,3-propanediol was increased by 19 % in the acoABCD mutant, with the disadvantage of lower substrate conversion ratio. Regulating the pyruvate acetyl-CoA switch presents a novel way to improve the conversion ratio or productivity of 1,3-propanediol produced by K. pneumoniae.

Jinjie Gu

Lab of Biorefinery,
Shanghai Advanced Research Institute,
Lab of Biorefinery, Shanghai Advanced Research Institute

Chinese Academy of Sciences

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Jinjie Gu

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Chinese Academy of Sciences

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Lab of Biorefinery,
Shanghai Advanced Research Institute,
Lab of Biorefinery, Shanghai Advanced Research Institute