droplet-barcoding-based-single-cell-transcriptomics-adult-mammalian

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

The analysis of single cell gene expression across thousands of individual cells within a tissue or microenvironment is a valuable tool for identifying ...

University of Calgary

Current methods for evaluating scar tissue volume following burns have shortcomings. The Vancouver Burn Scar scale is subjective, leading to a high variability in assessment. Although histological assessment via punch biopsy can discriminate between the different layers of skin, such an approach is invasive, inefficient, and detrimental to patient experience and wound healing. This study investigates the accuracy of high-frequency ultrasonography, a non-invasive alternative to histology, for measuring dermal and epidermal thickness in scar tissue.

Comparison between high-frequency ultrasonography and histological assessment reveals weak correlation for measurements of scar tissue thickness.

Current strategies for estimating positive scar volume following burns is limited to employing subjective scar scales. This study assesses the accuracy and reliability of Measurement of Area and Volume Instrument System (MAVIS) III, a portable 3D active stereophotogrammetric imaging system, for non-invasive assessment of hypertrophic scar volume and elucidates factors that leads to inaccurate volume determination.

Evaluating accuracy and reliability of active stereophotogrammetry using MAVIS III Wound Camera for three-dimensional assessment of hypertrophic scars.

RE: "Ultrasound is a reproducible and valid tool for measuring scar height in children with burn scars: A cross-sectional study of the psychometric properties and utility of the ultrasound and 3D camera" by M. Simons, E. Gee Kee, R. Kimble, Z. Tyack [Burns 43 (2017) 993-1001].

Sarthak Sinha

Department of Comparative Biology and Experimental Medicine,
Faculty of Veterinary Medicine,
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine

University of Calgary

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Sarthak Sinha

Department of Comparative Biology and Experimental Medicine,Faculty of Veterinary Medicine,Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine

University of Calgary

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Department of Comparative Biology and Experimental Medicine,
Faculty of Veterinary Medicine,
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine