Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
18.5K Views
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10:12 min
•
January 10th, 2019
DOI :
January 10th, 2019
•Jo Anne Stratton*1,2,3, Sarthak Sinha*2, Wisoo Shin2, Elodie Labit2, Tak-Ho Chu1,4, Prajay T. Shah2, Rajiv Midha1,4, Jeff Biernaskie1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
Chapters in this video
0:04
Title
0:56
Dissociating Tissue (Day 1)
3:46
Isolating Viable and Healthy Cells (Day 1)
4:46
GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
6:17
NCBI’s GEO and SRA Submissions
7:34
Results: Bioinformatics Analysis of Single Cell Transcriptomics
9:35
Conclusion
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