Virginia G. Piper Center for Personalized Diagnostics,
Biodesign Institute,
Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute
Executive Director, Biodesign Institute at Arizona State University
Director, Biodesign Institute’s Virginia G. Piper Center for Personalized Diagnostics; Dalton Endowed Chair of Cancer Research and Medicinal Chemistry;
Virginia G. Piper Chair of Personalized Medicine; Professor, School of Molecular Sciences; Arizona State University
Adjunct Professor of Medicine - College of Medicine, Mayo Clinic
Joshua LaBaer is one of the nation’s foremost investigators in the rapidly expanding field of personalized diagnostics. His efforts focus on the discovery and validation of biomarkers — unique molecular fingerprints of disease — which provide early warning for major illnesses, including cancer, infections and diabetes. Dr LaBaer leads a highly multidisciplinary staff of molecular biologists, cell biologists, biochemists, software engineers, database specialists, bioinformaticists, biostatisticians, and automation engineers. They employ open reading frame clones to the high throughput (HT) study of protein function. Their library of clones, DNASU, expects to achieve a complete set of full length human genes this year. Dr. LaBaer invented a novel protein microarray technology, Nucleic Acid Programmable Protein Array, which uses printed cDNAs as template to produce full-length folded proteins in situ. This technology has been used widely for biomedical research, including the discovery of a panel of 28 autoantibody biomarkers that led to Videssa™, the first CLIA-certified diagnostic test for the detection of breast cancer. Recent advances in the lab include the development of high-density arrays and multiplexed arrays. The use of human ribosomes and chaperone proteins has improved both the yield and natural folding of the proteins on the arrays, enabling the production of enzymatically active receptor tyrosine kinases on the arrays that could be screened with selective tyrosine kinase inhibitors.
Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays.
Infection and immunity Nov, 2009 | Pubmed ID: 19737893
Protein Structure Initiative Material Repository: an open shared public resource of structural genomics plasmids for the biological community.
Nucleic acids research Jan, 2010 | Pubmed ID: 19906724
p53 autoantibodies as potential detection and prognostic biomarkers in serous ovarian cancer.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology Mar, 2010 | Pubmed ID: 20200435
Use of a single-chain antibody library for ovarian cancer biomarker discovery.
Molecular & cellular proteomics : MCP Jul, 2010 | Pubmed ID: 20467042
Protein microarray signature of autoantibody biomarkers for the early detection of breast cancer.
Journal of proteome research Jan, 2011 | Pubmed ID: 20977275
High-throughput ectopic expression screen for tamoxifen resistance identifies an atypical kinase that blocks autophagy.
Proceedings of the National Academy of Sciences of the United States of America Feb, 2011 | Pubmed ID: 21233418
PSI:Biology-materials repository: a biologist's resource for protein expression plasmids.
Journal of structural and functional genomics Jul, 2011 | Pubmed ID: 21360289
Immunoprofiling using NAPPA protein microarrays.
Methods in molecular biology (Clifton, N.J.) , 2011 | Pubmed ID: 21370064
Nucleic Acid programmable protein arrays: versatile tools for array-based functional protein studies.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] Apr, 2011 | Pubmed ID: 21488044
Interferon-γ and proliferation responses to Salmonella enterica Serotype Typhi proteins in patients with S. Typhi Bacteremia in Dhaka, Bangladesh.
PLoS neglected tropical diseases Jun, 2011 | Pubmed ID: 21666798
Nucleic acid programmable protein array a just-in-time multiplexed protein expression and purification platform.
Methods in enzymology , 2011 | Pubmed ID: 21943897
Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays.
Molecular & cellular proteomics : MCP Feb, 2012 | Pubmed ID: 22311593
Circulating and synovial antibody profiling of juvenile arthritis patients by nucleic acid programmable protein arrays.
Arthritis research & therapy Apr, 2012 | Pubmed ID: 22510425
High density diffusion-free nanowell arrays.
Journal of proteome research Aug, 2012 | Pubmed ID: 22742968
Improving international research with clinical specimens: 5 achievable objectives.
Journal of proteome research Dec, 2012 | Pubmed ID: 22998582
A versatile protein microarray platform enabling antibody profiling against denatured proteins.
Proteomics. Clinical applications Jun, 2013 | Pubmed ID: 23027520
Robust microarray production of freshly expressed proteins in a human milieu.
Proteomics. Clinical applications Jun, 2013 | Pubmed ID: 23027544
Emerging protein array technologies for proteomics.
Expert review of proteomics Feb, 2013 | Pubmed ID: 23414360
High-throughput cloning and expression library creation for functional proteomics.
Proteomics May, 2013 | Pubmed ID: 23457047
Biomarker discovery for heterogeneous diseases.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology May, 2013 | Pubmed ID: 23462916
Quantifying antibody binding on protein microarrays using microarray nonlinear calibration.
BioTechniques May, 2013 | Pubmed ID: 23662896
Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children.
PloS one , 2013 | Pubmed ID: 23844187
Statistical design for biospecimen cohort size in proteomics-based biomarker discovery and verification studies.
Journal of proteome research Dec, 2013 | Pubmed ID: 24063748
DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research.
Nucleic acids research Jan, 2014 | Pubmed ID: 24225319
The Hippo transducer TAZ interacts with the SWI/SNF complex to regulate breast epithelial lineage commitment.
Cell reports Mar, 2014 | Pubmed ID: 24613358
Cellular and cytokine responses to Salmonella enterica serotype Typhi proteins in patients with typhoid fever in Bangladesh.
The American journal of tropical medicine and hygiene Jun, 2014 | Pubmed ID: 24615129
Development of a full-length human protein production pipeline.
Protein science : a publication of the Protein Society Aug, 2014 | Pubmed ID: 24806540
Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.
Theranostics , 2014 | Pubmed ID: 24955142
Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays.
Molecular & cellular proteomics : MCP Nov, 2014 | Pubmed ID: 25073739
Measurement of small molecule binding kinetics on a protein microarray by plasmonic-based electrochemical impedance imaging.
Analytical chemistry Oct, 2014 | Pubmed ID: 25153794
Recombinant expression, purification, and biophysical characterization of the transmembrane and membrane proximal domains of HIV-1 gp41.
Protein science : a publication of the Protein Society Nov, 2014 | Pubmed ID: 25155369
Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis.
mBio , 2014 | Pubmed ID: 25271291
AMPylation of Rho GTPases subverts multiple host signaling processes.
The Journal of biological chemistry Nov, 2014 | Pubmed ID: 25301945
In situ drug-receptor binding kinetics in single cells: a quantitative label-free study of anti-tumor drug resistance.
Scientific reports Oct, 2014 | Pubmed ID: 25312029
Autoantibody signature for the serologic detection of ovarian cancer.
Journal of proteome research Jan, 2015 | Pubmed ID: 25365139
Probing of CD4 binding pocket of HIV-1 gp120 glycoprotein using unnatural phenylalanine analogues.
Bioorganic & medicinal chemistry letters Dec, 2014 | Pubmed ID: 25453804
Kinome-level screening identifies inhibition of polo-like kinase-1 (PLK1) as a target for enhancing non-viral transgene expression.
Journal of controlled release : official journal of the Controlled Release Society Apr, 2015 | Pubmed ID: 25681050
Microreactor array device.
Scientific reports Mar, 2015 | Pubmed ID: 25736721
Host-pathogen interaction profiling using self-assembling human protein arrays.
Journal of proteome research Apr, 2015 | Pubmed ID: 25739981
Recent advances in proteomics applied to elucidate the role of environmental impacts on human health and organismal function.
Journal of proteome research Jan, 2015 | Pubmed ID: 25751307
Antiviral antibody profiling by high-density protein arrays.
Proteomics Jun, 2015 | Pubmed ID: 25758251
Site-specific recombinational cloning using gateway and in-fusion cloning schemes.
Current protocols in molecular biology Apr, 2015 | Pubmed ID: 25827088
High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays.
Nature protocols May, 2015 | Pubmed ID: 25881200
Plasma Autoantibodies Associated with Basal-like Breast Cancers.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology Sep, 2015 | Pubmed ID: 26070530
Nonenzymatic Protein Acetylation Detected by NAPPA Protein Arrays.
ACS chemical biology Sep, 2015 | Pubmed ID: 26083674
In Vitro Transcription/Translation System: A Versatile Tool in the Search for Missing Proteins.
Journal of proteome research Sep, 2015 | Pubmed ID: 26155874
Integration of Proteomics and Transcriptomics Data Sets for the Analysis of a Lymphoma B-Cell Line in the Context of the Chromosome-Centric Human Proteome Project.
Journal of proteome research Sep, 2015 | Pubmed ID: 26216070
Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion.
PloS one , 2015 | Pubmed ID: 26295457
Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays.
Diabetes Jan, 2016 | Pubmed ID: 26450993
Sustainability in a Hospital-Based Biobank and University-Based DNA Biorepository: Strategic Roadmaps.
Biopreservation and biobanking Dec, 2015 | Pubmed ID: 26697909
Advancing translational research with next-generation protein microarrays.
Proteomics Apr, 2016 | Pubmed ID: 26749402
Comparative Study of Autoantibody Responses between Lung Adenocarcinoma and Benign Pulmonary Nodules.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer Mar, 2016 | Pubmed ID: 26896032
Proteomic mapping of p53 immunogenicity in pancreatic, ovarian, and breast cancers.
Proteomics. Clinical applications 07, 2016 | Pubmed ID: 27121307
A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes.
Molecular & cellular proteomics : MCP 07, 2016 | Pubmed ID: 27141097
Mapping transcription factor interactome networks using HaloTag protein arrays.
Proceedings of the National Academy of Sciences of the United States of America 07, 2016 | Pubmed ID: 27357687
NAPPA as a Real New Method for Protein Microarray Generation.
Microarrays (Basel, Switzerland) Apr, 2015 | Pubmed ID: 27600221
Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays.
Journal of proteome research 01, 2017 | Pubmed ID: 27690455
AAgAtlas 1.0: a human autoantigen database.
Nucleic acids research 01, 2017 | Pubmed ID: 27924021
The Immune System and the Proteome.
Journal of proteome research 01, 2017 | Pubmed ID: 28056507
Identification of Antibody Against SNRPB, Small Nuclear Ribonucleoprotein-Associated Proteins B and B', as an Autoantibody Marker in Crohn's Disease using an Immunoproteomics Approach.
Journal of Crohn's & colitis Jul, 2017 | Pubmed ID: 28204086
Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays.
Molecular & cellular proteomics : MCP 04, 2017 | Pubmed ID: 28223349
Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.
Current protocols in cell biology Mar, 2017 | Pubmed ID: 28256722
Developing Human Radiation Biodosimetry Models: Testing Cross-Species Conversion Approaches Using an Ex Vivo Model System.
Radiation research 06, 2017 | Pubmed ID: 28328310
Autoantibody biomarkers for the detection of serous ovarian cancer.
Gynecologic oncology 07, 2017 | Pubmed ID: 28427776
Automatic Identification and Quantification of Extra-Well Fluorescence in Microarray Images.
Journal of proteome research 11, 2017 | Pubmed ID: 28938071
Multiplexed Nucleic Acid Programmable Protein Arrays.
Theranostics , 2017 | Pubmed ID: 29109798
Advances in cell-free protein array methods.
Expert review of proteomics 01, 2018 | Pubmed ID: 29214870
Expression system for structural and functional studies of human glycosylation enzymes.
Nature chemical biology 02, 2018 | Pubmed ID: 29251719
A Perspective on Proteomics of Infectious Diseases.
Proteomics. Clinical applications 07, 2018 | Pubmed ID: 29282898
Ibrutinib inhibition of ERBB4 reduces cell growth in a WNT5A-dependent manner.
Oncogene 04, 2018 | Pubmed ID: 29398709
Tandem Histone-Binding Domains Enhance the Activity of a Synthetic Chromatin Effector.
ACS synthetic biology 03, 2018 | Pubmed ID: 29429329
Legionella effector AnkX interacts with host nuclear protein PLEKHN1.
BMC microbiology 01, 2018 | Pubmed ID: 29433439
What's in a sample? Increasing transparency in biospecimen procurement methods.
Nature methods 04, 2018 | Pubmed ID: 29702632
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites.
Malaria journal Jul, 2018 | Pubmed ID: 30016987
A Noninvasive Blood-based Combinatorial Proteomic Biomarker Assay to Detect Breast Cancer in Women over age 50 with BI-RADS 3, 4, or 5 Assessment.
Clinical cancer research : an official journal of the American Association for Cancer Research 01, 2019 | Pubmed ID: 30185421
Launching the C-HPP neXt-CP50 Pilot Project for Functional Characterization of Identified Proteins with No Known Function.
Journal of proteome research 12, 2018 | Pubmed ID: 30269496
A Human Organotypic Microfluidic Tumor Model Permits Investigation of the Interplay between Patient-Derived Fibroblasts and Breast Cancer Cells.
Cancer research Jun, 2019 | Pubmed ID: 30992322
Multi-platform Approach for Microbial Biomarker Identification Using as a Model.
Frontiers in cellular and infection microbiology , 2019 | Pubmed ID: 31245298
Discovery of an autoantibody signature for the early diagnosis of knee osteoarthritis: data from the Osteoarthritis Initiative.
Annals of the rheumatic diseases Aug, 2019 | Pubmed ID: 31471297
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