protocols-3d-bioprinting-gelatin-methacryloyl-hydrogel-based

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Gelatin methacryloyl (GelMA) has become a popular biomaterial in the field of bioprinting. The derivation of this material is gelatin, which is hydrolyzed ...

Zhejiang University

To compare the outcomes between bilateral lateral rectus recession (BLR) and unilateral recession resection (R&R) procedures in the treatment of basic intermittent exotropia.

Bilateral lateral rectus recession versus unilateral recession resection for basic intermittent exotropia: a meta-analysis.

Hydrogel structures equipped with internal microchannels offer more in vivo-relevant models for construction of tissues and organs in vitro. However, currently used microfabrication methods of constructing microfluidic devices are not suitable for the handling of hydrogel. This study presents a novel method of fabricating hydrogel-based microfluidic chips by combining the casting and bonding processes. A twice cross-linking strategy is designed to obtain a bonding interface that has the same strength with the hydrogel bulk, which can be applied to arbitrary combinations of hydrogels. It is convenient to achieve the construction of hydrogel structures with channels in branched, spiral, serpentine, and multilayer forms. The experimental results show that the combination of gelatin and gelatin methacrylate (GelMA) owns the best biocompatibility and can promote cell functionalization. Based on these, a vessel-on-a-chip system with vascular function in both physiological and pathological situations is established, providing a promising model for further investigations such as vascularization, vascular inflammation, tissue engineering, and drug development. Taken together, a facile and cytocompatible approach is developed for engineering a user-defined hydrogel-based chip that can be potentially useful in developing vascularized tissue or organ models.

Vessel-on-a-chip with Hydrogel-based Microfluidics.

A novel entity of acute myeloid leukaemia with recurrent RARG-rearrangement resembling acute promyelocytic leukaemia.

In this work, (AuNiPd₄)Ti₄ (wt.%) filler alloy was designed and employed to join SiC ceramics. The effects of brazing temperature and soaking time on the microstructure and fracture morphology of joints were investigated. The results show that the joint obtained can be described as SiC/reaction layer/braze/reaction layer/SiC. The reaction layer was composed of TiC and Au (Si, Ti). The wettability of the filler alloy toward the SiC ceramics was analyzed. The braze zone was mainly constituted by Pd₂Si, Ni₂Si, and Au (Ni, Si). A large number of nano-sized TiC particles were distributed within the Au (Ni, Si) layer. The formation mechanism of the braze containing different phases was discussed. The brazing temperature and soaking time had a significant effect on the reaction layer at the SiC/braze interface and TiC particles within the Au (Ni, Si) layer, while they showed a negligible effect on the Pd₂Si and Ni₂Si within the braze. The inherent reason was also clarified in detail. The joint fractography indicated that a good bonding was achieved between the filler alloy and SiC, while joint fracture was primarily induced by the thermal stresses residing after the brazing cycle.

Characterization of SiC Ceramic Joints Brazed Using Au⁻Ni⁻Pd⁻Ti High-Temperature Filler Alloy.

A Ni-17Mo-7Cr-based superalloy was laser surface-modified to improve its tribological properties. Si particles were employed as coating materials. Si melted on the surface of the alloy during the process, triggering the formation of MoNiC carbides and Ni-Si intermetallics. A defect-free coating obtained was mostly made up of primary MoNiC and γ-NiSi, as well as a eutectic structure of β-NiSi and α-Ni-based solid solution (α-Ni (s.s)). The volume fraction of hard reinforcements (MoNiC, γ-NiSi, and β-NiSi) reached up to 85% in the coating. High-temperature microstructural stability of the coating was investigated by aging the coating at 1073 K for 240-480 h, to reveal its microstructural evolution. In addition, the mechanical performance of the coating was investigated. The nanoscale elastic modulus and hardness of MoNiC, γ-NiSi, and α-Ni (s.s) were characterized using the nanoindentation tests. The nanoscratch tests were performed to measure the local wear resistance of the coating. Lastly, the Vickers hardness distribution across the cross-section of the coating before and after thermal exposure was compared. The work performed provides basic information understanding the microstructural evolution and mechanical performance of laser-induced coatings on Ni-based superalloys.

Microstructural Evolution and Mechanical Evaluation of a Laser-Induced Composite Coating on a Ni-Based Superalloy during Thermal Exposure.

To evaluate the corneal wound healing response after small incision lenticule extraction surgery.

Early Corneal Wound Healing Response After Small Incision Lenticule Extraction.

Mucosal immunization has potential benefits over conventional parenteral immunization, eliciting immune defense in both mucosal and systemic tissue for protecting from pathogen invasion at mucosal surfaces. To provide a first line of protection at these entry ports, mucosal vaccines have been developed and hold a significant promise for reducing the burden of infectious diseases. However, until very recently, only limited mucosal vaccines are available. This review summarizes recent advances in selected aspects regarding mucosal vaccination, including appropriate administration routes, reasonable formulations, antigen-sampling and immune responses of mucosal immunity, and the strategies used to improve mucosal vaccine efficacy. Finally, the challenges of developing successful mucosal vaccines and the potential solutions are discussed.

Yuan Sun

Zhejiang University

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks