Tiago G. Fernandes

iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering,
Instituto Superior Técnico,
iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico

Universidade de Lisboa

BIOGRAPHY

Dr. Tiago G. Fernandes completed a PhD in Biotechnology in 2009 at Universidade de Lisboa, Instituto Superior Técnico, and a "Licenciatura" degree in Biological Engineering in 2004 in the same Alma Mater. He is an assistant professor at Instituto Superior Técnico, Universidade de Lisboa, and a research scientist at the Institute for Bioengineering and Biosciences, Lisbon, Portugal. He held a temporary position as invited visiting professor at the Department of Biomedical Engineering and Wisconsin Institute for Discovery, University of Wisconsin-Madison, USA, in 2020. His work is focused on providing an integrated platform that brings together engineering and biology in order to accelerate progress towards designing the stem cell fate and its microenvironment. The development of artificial cellular niches for studying the mechanisms that affect human stem cell pluripotency is of foremost importance and represents a major goal of his research. Human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are used as model systems to establish in vitro artificial microenvironments that better mimic the extracellular environment. In addition to the influence of mechanical and matrix-related responses, the effects of microenvironmental conditions (e.g. morphogens) have been extensively studied due to their capacity to modulating intracellular pathways. This systems-based approach is expected to contribute to the development of innovative cell culture models that better reflect in vivo function, and the final goal is to generate cells and tissues to advance our understanding of biology and tissue regeneration, which will lead to further development of cell-based therapies.

PUBLICATIONS

Scalable expansion of human-induced pluripotent stem cells in xeno-free microcarriers.

Methods in molecular biology (Clifton, N.J.) , 2015 | Pubmed ID: 25108454

Purification of human induced pluripotent stem cell-derived neural precursors using magnetic activated cell sorting.

Methods in molecular biology (Clifton, N.J.) , 2015 | Pubmed ID: 25537837

Spatial and temporal control of cell aggregation efficiently directs human pluripotent stem cells towards neural commitment.

Biotechnology journal Oct, 2015 | Pubmed ID: 25866360

Neural commitment of human pluripotent stem cells under defined conditions recapitulates neural development and generates patient-specific neural cells.

Biotechnology journal Oct, 2015 | Pubmed ID: 26123315

Engineering at the microscale: A step towards single-cell analysis of human pluripotent stem cells.

Biotechnology journal Oct, 2015 | Pubmed ID: 26189940

Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems.

PloS one , 2016 | Pubmed ID: 26999816

Microcarrier-based platforms for in vitro expansion and differentiation of human pluripotent stem cells in bioreactor culture systems.

Journal of biotechnology Sep, 2016 | Pubmed ID: 27480342

Scaling up a chemically-defined aggregate-based suspension culture system for neural commitment of human pluripotent stem cells.

Biotechnology journal Dec, 2016 | Pubmed ID: 27754603

Biophysical study of human induced Pluripotent Stem Cell-Derived cardiomyocyte structural maturation during long-term culture.

Biochemical and biophysical research communications 05, 2018 | Pubmed ID: 29601816

Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironments.

Methods in molecular biology (Clifton, N.J.) , 2018 | Pubmed ID: 29633205

A scale out approach towards neural induction of human induced pluripotent stem cells for neurodevelopmental toxicity studies.

Toxicology letters Sep, 2018 | Pubmed ID: 29775723

Towards Multi-Organoid Systems for Drug Screening Applications.

Bioengineering (Basel, Switzerland) Jun, 2018 | Pubmed ID: 29933623

Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering.

Stem cells international , 2019 | Pubmed ID: 31149014

Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture.

Scientific reports 06, 2019 | Pubmed ID: 31239450

Engineering Cell Systems.

Stem cells international , 2019 | Pubmed ID: 31281374

Affinity-Triggered Assemblies Based on a Designed Peptide-Peptide Affinity Pair.

Biotechnology journal Nov, 2019 | Pubmed ID: 31283091

Multifactorial Modeling Reveals a Dominant Role of Wnt Signaling in Lineage Commitment of Human Pluripotent Stem Cells.

Bioengineering (Basel, Switzerland) Aug, 2019 | Pubmed ID: 31443254

Human Pluripotent Stem Cells: Applications and Challenges for Regenerative Medicine and Disease Modeling.

Advances in biochemical engineering/biotechnology , 2020 | Pubmed ID: 31740987

Angelman syndrome: a journey through the brain.

The FEBS journal Jun, 2020 | Pubmed ID: 32087041

Maturation of Human Pluripotent Stem Cell-Derived Cerebellar Neurons in the Absence of Co-culture.

Frontiers in bioengineering and biotechnology , 2020 | Pubmed ID: 32117945

Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications.

Bioengineering (Basel, Switzerland) Apr, 2020 | Pubmed ID: 32283585

INSTITUTIONS

Universidade de Lisboa

JOVE ARTICLES

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

Teresa P. Silva^1,2,

Tiago G. Fernandes¹,

Diogo E. S. Nogueira¹,

Carlos A. V. Rodrigues¹,

Evguenia P. Bekman^1,2,3,

Yas Hashimura⁴,

Sunghoon Jung⁴,

Brian Lee⁴,

Maria Carmo-Fonseca²,

Joaquim M. S. Cabral¹

¹iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa,

²Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa,

³The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa,

⁴, PBS Biotech, Inc, Camarillo, CA, USA

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