application-flow-vermimetry-for-quantification-analysis

Application of Flow Vermimetry for Quantification and Analysis of the <em>Caenorhabditis elegans</em> Gut Microbiome

The composition of the gut microbiome can have a dramatic impact on host physiology throughout the development and the life of the animal. Measuring ...

Baylor College of Medicine

Hydrogen peroxide (H2O2) is the most common chemical threat that organisms face. Here, we show that H2O2 alters the bacterial food preference of Caenorhabditis elegans, enabling the nematodes to find a safe environment with food. H2O2 induces the nematodes to leave food patches of laboratory and microbiome bacteria when those bacterial communities have insufficient H2O2-degrading capacity. The nematode's behavior is directed by H2O2-sensing neurons that promote escape from H2O2 and by bacteria-sensing neurons that promote attraction to bacteria. However, the input for H2O2-sensing neurons is removed by bacterial H2O2-degrading enzymes and the bacteria-sensing neurons' perception of bacteria is prevented by H2O2. The resulting cross-attenuation provides a general mechanism that ensures the nematode's behavior is faithful to the lethal threat of hydrogen peroxide, increasing the nematode's chances of finding a niche that provides both food and protection from hydrogen peroxide.

Modulation of sensory perception by hydrogen peroxide enables Caenorhabditis elegans to find a niche that provides both food and protection from hydrogen peroxide.

Ciara N. Hosea

Alkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology,
Development,
Disease Models and Therapeutics Program,
Graduate School of Biomedical Sciences,
Development, Disease Models and Therapeutics Program, Graduate School of Biomedical Sciences

Baylor College of Medicine

Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

Ciara N. Hosea

Alkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology,Development,Disease Models and Therapeutics Program,Graduate School of Biomedical Sciences,Development, Disease Models and Therapeutics Program, Graduate School of Biomedical Sciences

Baylor College of Medicine

Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

Alkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology,
Development,
Disease Models and Therapeutics Program,
Graduate School of Biomedical Sciences,
Development, Disease Models and Therapeutics Program, Graduate School of Biomedical Sciences