a-stable-phantom-material-for-optical-and-acoustic-imaging

A Stable Phantom Material for Optical and Acoustic Imaging

Establishing tissue-mimicking biophotonic phantom materials that provide long-term stability are imperative to enable the comparison of biomedical imaging ...

National Physical Laboratory

To support the development of clinical applications of high-frequency ultrasound, appropriate tissue-mimicking materials (TMMs) are required whose acoustic properties have been measured using validated techniques. This paper describes the characterisation of the sound speed (phase velocity) and attenuation coefficient of the International Electrotechnical Commission (IEC) agar-based TMM over the frequency range 1 to 60 MHz. Measurements implemented a broadband through-transmission substitution immersion technique over two overlapping frequency ranges, with co-axially aligned 50 MHz centre-frequency transducers employed for characterisation above 15 MHz. In keeping with usual practice employed within the technical literature, thin acoustic windows (membranes) made of 12-μm-thick Mylar protected the TMM from water damage. Various important sources of uncertainty that could compromise measurement accuracy have been identified and evaluated through a combination of experimental studies and modelling. These include TMM sample thickness, measured both manually and acoustically, and the influence of interfacial losses that, even for thin protective membranes, are significant at the frequencies of interest. In agreement with previous reports, the attenuation coefficient of the IEC TMM exhibited non-linear frequency dependence, particularly above 20 MHz, yielding a value of 0.93 ± 0.04 dB cm(-1) MHz(-1) at 60 MHz, derived at 21 ± 0.5°C. For the first time, phase velocity, measured with an estimated uncertainty of ±3.1 m s(-1), has been found to be dispersive over this extended frequency range, increasing from 1541 m s(-1) at 1 MHz to 1547 m s(-1) at 60 MHz. This work will help standardise acoustic property measurements, and establishes a reference measurement capability for TMMs underpinning clinical applications at elevated frequencies.

Reference characterisation of sound speed and attenuation of the IEC agar-based tissue-mimicking material up to a frequency of 60 MHz.

The acoustic properties of a robust tissue-mimicking material based on konjac–carrageenan at ultrasound frequencies in the range 5–60 MHz are described. Acoustic properties were characterized using two methods: a broadband reflection substitution technique using a commercially available preclinical ultrasound scanner (Vevo 770, FUJIFILM VisualSonics, Toronto, ON, Canada), and a dedicated high-frequency ultrasound facility developed at the National Physical Laboratory (NPL, Teddington, UK), which employed a broadband through-transmission substitution technique. The mean speed of sound across the measured frequencies was found to be 1551.7 ± 12.7 and 1547.7 ± 3.3 m s21, respectively. The attenuation exhibited a non-linear dependence on frequency, f (MHz), in the form of a polynomial function: 0.009787f2 1 0.2671f and 0.01024f2 1 0.3639f, respectively. The characterization of this tissue-mimicking material will provide reference data for designing phantoms for preclinical systems, which may, in certain applications such as flow phantoms, require a physically more robust tissuemimicking material than is currently available.

acoustic assessment of a konjac–carrageenan tissue-mimicking material aT 5–60 MHZ.

The characterization of ultrasound fields generated by diagnostic and therapeutic equipment is an essential requirement for performance validation and to demonstrate compliance against established safety limits. This requires hydrophones calibrated to a traceable standard. Currently, the upper calibration frequency range available to the user community is limited to 60 MHz. However, high frequencies are increasingly being used for both imaging and therapy necessitating calibration frequencies up to 100 MHz. The precise calibration of hydrophones requires a source of high amplitude, broadband, quasi-planar, and stable ultrasound fields. There are challenges to using conventional piezoelectric sources, and laser generated ultrasound sources offer a promising solution. In this study, various nanocomposites consisting of a bulk polymer matrix and multi-walled carbon nanotubes were fabricated and tested using pulsed laser of a few nanoseconds for their suitability as a source for high frequency calibration of hydrophones. The pressure amplitude and bandwidths were measured using a broadband hydrophone from 27 different nanocomposite sources. The effect of nonlinear propagation of high amplitude laser generated ultrasound on bandwidth and the effect of bandlimited sensitivity response on the deconvolved pressure waveform were numerically investigated. The stability of the nanocomposite sources under sustained laser pulse excitation was also examined.

Laser generated ultrasound sources using carbon-polymer nanocomposites for high frequency metrology.

High-frequency calibration of hydrophones is becoming increasingly important, both for clinical and scientific applications of ultrasound, and user safety. At present, the calibrations available routinely to the user community extend to 60 MHz. However, hydrophones that can measure beyond this are available, and ultrasonic fields often contain energy at higher frequencies, e.g., generated through nonlinear propagation of high-amplitude ultrasound used for therapeutic applications, and the increasing use of higher frequencies in imaging. Therefore, there is a need for calibrations up to at least 100 MHz, to allow ultrasonic fields to be accurately characterized, and the risk of harmful bioeffects to be properly assessed. Currently, sets of focused piezoelectric transducers are used to meet the pressure amplitude and bandwidth requirements of Primary Standard calibration facilities. However, when the frequency is high enough such that the size of the ultrasound focus becomes less than the hydrophone element's diameter, the uncertainty due to spatial averaging becomes significant, and can be as high as 20% at 100 MHz. As an alternate to piezoelectric transducers, a laser-generated ultrasound calibration source was designed, fabricated, and characterized. The source consists of an optically absorbing carbon-polymer nanocomposite excited by a large-diameter 1064 nm laser pulse of 2.6 ns duration. Peak pressure amplitudes of several Mega-Pascal were readily achievable, and the signal contained measurable frequency components up to 100 MHz. The variation in the pressure amplitudes was less than 2% from its mean over a three-hour test period. The ultrasound beam was sufficiently broad that the uncertainties due to spatial averaging were negligible.

100 MHz bandwidth planar laser-generated ultrasound source for hydrophone calibration.

The effect of temperature and electrical drive conditions on the output of lead zirconate titanate (PZT) transducers is of particular interest in ultrasound metrology and medical ultrasound applications. In this work, the temperature-dependent output of two single-element PZT transducers was measured between 22 °C and 46 °C. Two independent measurement methods were used, namely radiation force balance measurements and laser vibrometry. When driven at constant voltage using a 50 Ω matched signal generator and amplifier using continuous wave (CW) or quasi-CW excitation, the output of the two transducers increased on average by 0.6 % per degree, largely due to an increase in transducer efficiency with temperature. The two measurement methods showed close agreement. Similar trends were observed when using single cycle excitation with the same signal chain. However, when driven using a pulser (which is not electrically matched), the two transducers exhibited different behaviour depending on their electrical impedance. Accounting for the temperature-dependent output of PZT transducers could have implications for many areas of ultrasound metrology, for example, in therapeutic ultrasound where a coupling fluid at an increased or decreased temperature is often used.

Measurement of the temperature-dependent output of lead zirconate titanate transducers.

Optical generation of ultrasound using nanosecond duration laser pulses has generated great interest both in industrial and biomedical applications. The availability of portable laser devices using semiconductor technology and optical fibres, as well as numerous source material types based on nanocomposites, has proliferated the applications of laser ultrasound. The nanocomposites can be deposited on the tip of optical fibres as well as planar hard and soft backing materials using various fabrication techniques, making devices suitable for a variety of applications. The ability to choose the acoustic material properties and the laser pulse duration gives considerable control over the ultrasound output. Here, an analytical time-domain solution is derived for the acoustic pressure waveform generated by a planar optical ultrasound source consisting of an optically absorbing layer on a backing. It is shown that by varying the optical attenuation coefficient, the thickness of the absorbing layer, the acoustic properties of the materials, and the laser pulse duration, a wide variety of pulse shapes and trains can be generated. It is shown that a source with a reflecting backing can generate pulses with higher amplitude than a source with an acoustically-matched backing in the same circumstances when stress-confinement has not been satisfied.

Modelling laser ultrasound waveforms: The effect of varying pulse duration and material properties.

In this article, it is shown experimentally that a planar laser-generated ultrasound source with a hard reflective backing will generate higher acoustic pressures than a comparable source with an acoustically matched backing when the stress confinement condition is not met. Furthermore, while the source with an acoustically matched backing will have a broader bandwidth when the laser pulse is short enough to ensure stress confinement, the bandwidths of both source types will converge as the laser pulse duration increases beyond stress confinement. The explanation of the results is supported by numerical simulations.

Article	Year
Reference characterisation of sound speed and attenuation of the IEC agar-based tissue-mimicking material up to a frequency of 60 MHz. Ultrasound in medicine & biology\| PubMed ID: 25220268	2015
acoustic assessment of a konjac–carrageenan tissue-mimicking material aT 5–60 MHZ. Ultrasound in medicine & biology\| PubMed ID: 25438864	2014
Laser generated ultrasound sources using carbon-polymer nanocomposites for high frequency metrology. The Journal of the Acoustical Society of America\| PubMed ID: 30180662	2018
100 MHz bandwidth planar laser-generated ultrasound source for hydrophone calibration. Ultrasonics\| PubMed ID: 32721650	2020
Measurement of the temperature-dependent output of lead zirconate titanate transducers. Ultrasonics\| PubMed ID: 33582459	2021
Modelling laser ultrasound waveforms: The effect of varying pulse duration and material properties. The Journal of the Acoustical Society of America\| PubMed ID: 33765774	2021
The effect of source backing materials and excitation pulse durations on laser-generated ultrasound waveforms. The Journal of the Acoustical Society of America\| PubMed ID: 37129678	2023

Srinath Rajagopal

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