generating-human-pancreatic-tissue-slices-to-study-endocrine-exocrine

Generating Human Pancreatic Tissue Slices to Study Endocrine and Exocrine Pancreas Physiology

It is crucial to study the human pancreas to understand the pathophysiological mechanisms associated with type 1 (T1D) and 2 diabetes (T2D) as well as the ...

Arthur Riggs Diabetes and Metabolism Research Institute

Pancreatic tissue slices allow functional investigations under close physiological conditions in situ. This approach is particularly advantageous for studying infiltrated and structurally damaged islets as found in T1D. More importantly, slices allow studying the interplay between endocrine and exocrine compartments. We here describe how to perform agarose injections, tissue preparation, and slice procedure for mouse and human tissue. We then describe in detail how to use the slices to perform functional studies using hormone secretion and calcium imaging as readouts. For complete details on the use and execution of this protocol, please refer to Panzer et al. (2022)..

Julia K. Panzer

Department of Diabetes Immunology

Arthur Riggs Diabetes and Metabolism Research Institute

Generating Human Pancreatic Tissue Slices to Study Endocrine and Exocrine Pancreas Physiology

Julia K. Panzer

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Diabetes Immunology

Arthur Riggs Diabetes and Metabolism Research Institute

Generating Human Pancreatic Tissue Slices to Study Endocrine and Exocrine Pancreas Physiology

Department of Diabetes Immunology