Patrick J. Hrdlicka

Functionalized 2'-amino-alpha-L-LNA: directed positioning of intercalators for DNA targeting.

C5-functionalized LNA: unparalleled hybridization properties and enzymatic stability.

Invader LNA: efficient targeting of short double stranded DNA.

Glowing locked nucleic acids: brightly fluorescent probes for detection of nucleic acids in cells.

Novel insights into the use of Glowing LNA as nucleic acid detection probes--influence of labeling density and nucleobases.

C5-Functionalized DNA, LNA, and α-L-LNA: Positional Control of Polarity-Sensitive Fluorophores Leads to Improved SNP-Typing.

Preparation of C5-functionalized locked nucleic acids (LNAs).

Pyrene-functionalized oligonucleotides and locked nucleic acids (LNAs): tools for fundamental research, diagnostics, and nanotechnology.

High-affinity DNA targeting using readily accessible mimics of N2'-functionalized 2'-amino-α-L-LNA.

Gold-peptide nanoconjugate cellular uptake is modulated by serum proteins.

C2'-pyrene-functionalized triazole-linked DNA: universal DNA/RNA hybridization probes.

DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles.

Fluorescent intercalator displacement replacement (FIDR) assay: determination of relative thermodynamic and kinetic parameters in triplex formation--a case study using triplex-forming LNAs.

Synthesis and hybridization properties of oligonucleotides modified with 5-(1-aryl-1,2,3-triazol-4-yl)-2'-deoxyuridines.

DNA strands with alternating incorporations of LNA and 2'--(pyren-1-yl)methyluridine: SNP-discriminating RNA detection probes.

Identification and characterization of second-generation invader locked nucleic acids (LNAs) for mixed-sequence recognition of double-stranded DNA.

Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens.

Invaders: Recognition of Double-Stranded DNA by Using Duplexes Modified with Interstrand Zippers of 2'-O-(Pyren-1-yl)methyl-ribonucleotides.

Recognition of mixed-sequence DNA duplexes: design guidelines for invaders based on 2'-O-(pyren-1-yl)methyl-RNA monomers.

Synthesis and characterization of oligodeoxyribonucleotides modified with 2'-amino-α-L-LNA adenine monomers: high-affinity targeting of single-stranded DNA.

C5-alkynyl-functionalized α-L-LNA: synthesis, thermal denaturation experiments and enzymatic stability.

Synthesis and biophysical properties of C5-functionalized LNA (locked nucleic acid).

Carbohydrate-functionalized locked nucleic acids: oligonucleotides with extraordinary binding affinity, target specificity, and enzymatic stability.

Synthesis, hybridization characteristics, and fluorescence properties of oligonucleotides modified with nucleobase-functionalized locked nucleic acid adenosine and cytidine monomers.

C5-amino acid functionalized LNA: positively poised for antisense applications.

Self-assembled monolayers of thiols adsorbed on Au/ZnO-functionalized silica nanosprings: photoelectron spectroscopy-analysis and detection of vaporized explosives.

Recognition of double-stranded DNA using energetically activated duplexes with interstrand zippers of 1-, 2- or 4-pyrenyl-functionalized O2'-alkylated RNA monomers.

Recognition of Double-Stranded DNA Using Energetically Activated Duplexes Modified with N2'-Pyrene-, Perylene-, or Coronene-Functionalized 2'-N-Methyl-2'-amino-DNA Monomers.

Locked nucleic acid (LNA) induced effect on the hybridization and fluorescence properties of oligodeoxyribonucleotides modified with nucleobase-functionalized DNA monomers.

Mixed-Sequence Recognition of Double-Stranded DNA Using Enzymatically Stable Phosphorothioate Invader Probes.

Invader probes: Harnessing the energy of intercalation to facilitate recognition of chromosomal DNA for diagnostic applications.

Synthesis and characterization of oligodeoxyribonucleotides modified with 2'-thio-2'-deoxy-2'-S-(pyren-1-yl)methyluridine.

Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles.

Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling.

Next-generation bis-locked nucleic acids with stacking linker and 2'-glycylamino-LNA show enhanced DNA invasion into supercoiled duplexes.

Efficient Discrimination of Single Nucleotide Polymorphisms (SNPs) Using Oligonucleotides Modified with C5-Pyrene-Functionalized DNA and Flanking Locked Nucleic Acid (LNA) Monomers.

Merging Two Strategies for Mixed-Sequence Recognition of Double-Stranded DNA: Pseudocomplementary Invader Probes.

Recognition of mixed-sequence DNA using double-stranded probes with interstrand zipper arrangements of O2'-triphenylene- and coronene-functionalized RNA monomers.

25 years and still going strong: 2'-O-(pyren-1-yl)methylribonucleotides - versatile building blocks for applications in molecular biology, diagnostics and materials science.

Synthesis and biophysical characterization of oligonucleotides modified with O2'-alkylated RNA monomers featuring substituted pyrene moieties.

Recognition of mixed-sequence DNA targets using spermine-modified Invader probes.

Head-to-head comparison of LNA, γPNA, INA and Invader probes targeting mixed-sequence double-stranded DNA.

Chimeric γPNA-Invader probes: using intercalator-functionalized oligonucleotides to enhance the DNA-targeting properties of γPNA.

Impact of non-nucleotidic bulges on recognition of mixed-sequence dsDNA by pyrene-functionalized Invader probes.

CTCF binding modulates UV damage formation to promote mutation hot spots in melanoma.

Recognition of double-stranded DNA using LNA-modified toehold Invader probes.

Nicked Invader probes: multistranded and sequence-unrestricted recognition of double-stranded DNA.

Serine-γPNA, Invader probes, and chimeras thereof: three probe chemistries that enable sequence-unrestricted recognition of double-stranded DNA.

Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA.

Recognition of mixed-sequence double-stranded DNA regions using chimeric Invader/LNA probes.

Conformation matters: siRNAs with antisense strands with 5'-()-vinyl-phosphonate-α-L-LNA elicit stronger RNAi-mediated gene silencing than those with 5'-()-vinyl-phosphonate-LNA.