Pamela A. Silver

Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling.

Protein and RNA export from the nucleus.

The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress.

Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis.

Identifying proteins that affect mRNA localization in living cells.

Nuclear protein transport.

Requirements for the nuclear export of the small ribosomal subunit.

Retrovirus-delivered siRNA.

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import.

Intron status and 3'-end formation control cotranscriptional export of mRNA.

In situ analysis of spatial relationships between proteins of the nuclear pore complex.

Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae.

Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery.

A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry.

Nuclear transport as a target for cell growth.

Sac3 is an mRNA export factor that localizes to cytoplasmic fibrils of nuclear pore complex.

Analysis of RNA-protein interactions by flow cytometry.

Intracellular transport of human immunodeficiency virus type 1 integrase.

A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.

Small molecule modulation of the human chromatid decatenation checkpoint.

A chemical genetic screen identifies inhibitors of regulated nuclear export of a Forkhead transcription factor in PTEN-deficient tumor cells.

Modulation of interprotomer relationships is important for activation of dimeric calcium-sensing receptor.

Nuclear transport and cancer: from mechanism to intervention.

Therapeutic potential of retroviral RNAi vectors.

Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases.

Guardian at the gate: preventing unspliced pre-mRNA export.

Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.

PRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits.

REF-ereeing the cytoplasmic fate of mRNA via nuclear export.

Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors.

Human cell proteins and human immunodeficiency virus DNA integration.

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase.

Genome-wide mRNA surveillance is coupled to mRNA export.

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication.

A systems view of mRNP biology.

HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases.

The psammaplysenes, specific inhibitors of FOXO1a nuclear export.

Developmentally induced changes in transcriptional program alter spatial organization across chromosomes.

Ufd1 exhibits the AAA-ATPase fold with two distinct ubiquitin interaction sites.

Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.

Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells.

Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p.

The uses of green fluorescent protein in yeasts.

Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication.

Autoregulation of ribosome biosynthesis by a translational response in fission yeast.

Genomic association of the proteasome demonstrates overlapping gene regulatory activity with transcription factor substrates.

Motion as a phenotype: the use of live-cell imaging and machine visual screening to characterize transcription-dependent chromosome dynamics.

Pore-ing the right dose.

Systems biology of gene regulation fulfills its promise.

Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription.

The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.

Transcriptional regulation by the proteasome as a mechanism for cellular protein homeostasis.

Solenopsin, the alkaloidal component of the fire ant (Solenopsis invicta), is a naturally occurring inhibitor of phosphatidylinositol-3-kinase signaling and angiogenesis.

Genome-wide analysis of estrogen receptor binding sites.

The unfolded protein response transducer Ire1p contains a nuclear localization sequence recognized by multiple beta importins.

Communication between levels of transcriptional control improves robustness and adaptivity.

Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors.

The role of protein arginine methylation in the formation of silent chromatin.

Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing.

Designing biological systems.

Transcriptional regulation at the nuclear pore complex.

Why do so few women speak at science meetings?

Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases.

Systems perspectives on mRNA processing.

Exon expression profiling reveals stimulus-mediated exon use in neural cells.

Rational design of memory in eukaryotic cells.

Functional specificity among ribosomal proteins regulates gene expression.

Coupling and coordination in gene expression processes: a systems biology view.

Global analysis of mRNA splicing.

Definition of global and transcript-specific mRNA export pathways in metazoans.

CARM1 regulates estrogen-stimulated breast cancer growth through up-regulation of E2F1.

Defossiling fuel: how synthetic biology can transform biofuel production.

Enhancement of cell type specificity by quantitative modulation of a chimeric ligand.

Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes.

Intron delays and transcriptional timing during development.

Intron length increases oscillatory periods of gene expression in animal cells.

mRNA nuclear export and human disease.

Learning a prior on regulatory potential from eQTL data.

Engineering a synthetic dual-organism system for hydrogen production.

Harnessing nature's toolbox: regulatory elements for synthetic biology.

A conserved CCCH-type zinc finger protein regulates mRNA nuclear adenylation and export.

Synthetic biology: exploring and exploiting genetic modularity through the design of novel biological networks.

Systems-level engineering of nonfermentative metabolism in yeast.

Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and -independent mechanisms.

Eukaryotic systems broaden the scope of synthetic biology.

Anti-glycophorin single-chain Fv fusion to low-affinity mutant erythropoietin improves red blood cell-lineage specificity.

Making cellular memories.

A synthetic circuit for selectively arresting daughter cells to create aging populations.

Insulation of a synthetic hydrogen metabolism circuit in bacteria.

Spatially ordered dynamics of the bacterial carbon fixation machinery.

Engineering cyanobacteria to synthesize and export hydrophilic products.

Knowing when to change: reprogramming (my) life.

An alternative splicing network links cell-cycle control to apoptosis.

Emergent cooperation in microbial metabolism.

A structurally tunable DNA-based extracellular matrix.

Genome-wide RNAi screen discovers functional coupling of alternative splicing and cell cycle control to apoptosis regulation.

Dynamics in the mixed microbial concourse.

Engineering cyanobacteria to generate high-value products.

Synthetic circuit identifies subpopulations with sustained memory of DNA damage.

Rewiring hydrogenase-dependent redox circuits in cyanobacteria.

Informing biological design by integration of systems and synthetic biology.

Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain.

Modular electron transfer circuits for synthetic biology: insulation of an engineered biohydrogen pathway.

Towards a synthetic chloroplast.

A synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolism.

Visualization of single mRNAs reveals temporal association of proteins with microRNA-regulated mRNA.

Synthetic reversal of epigenetic silencing.

Recording cellular experiences of DNA damage.

Organization of intracellular reactions with rationally designed RNA assemblies.

Cell-to-cell variability of alternative RNA splicing.

Parts plus pipes: synthetic biology approaches to metabolic engineering.

Modularity of a carbon-fixing protein organelle.

Rerouting carbon flux to enhance photosynthetic productivity.

A tunable zinc finger-based framework for Boolean logic computation in mammalian cells.

Induction of biogenic magnetization and redox control by a component of the target of rapamycin complex 1 signaling pathway.

A sensitive switch for visualizing natural gene silencing in single cells.

DNA ends alter the molecular composition and localization of Ku multicomponent complexes.

Engineering synthetic TAL effectors with orthogonal target sites.

Natural strategies for the spatial optimization of metabolism in synthetic biology.

Improving carbon fixation pathways.

A BioBrick compatible strategy for genetic modification of plants.

STAR RNA-binding protein Quaking suppresses cancer via stabilization of specific miRNA.

Synthetic memory circuits for tracking human cell fate.

Designing biological compartmentalization.

Designing and using RNA scaffolds to assemble proteins in vivo.

Spatial and temporal organization of chromosome duplication and segregation in the cyanobacterium Synechococcus elongatus PCC 7942.

Expression of the sub-pathways of the Chloroflexus aurantiacus 3-hydroxypropionate carbon fixation bicycle in E. coli: Toward horizontal transfer of autotrophic growth.

PQBP1, a factor linked to intellectual disability, affects alternative splicing associated with neurite outgrowth.

Protein scaffold-activated protein trans-splicing in mammalian cells.

Tailored fatty acid synthesis via dynamic control of fatty acid elongation.

Dynamics simulations for engineering macromolecular interactions.

Isothermal self-assembly of complex DNA structures under diverse and biocompatible conditions.

Two- and three-input TALE-based AND logic computation in embryonic stem cells.

Targeting a heterologous protein to multiple plant organelles via rationally designed 5' mRNA tags.

The bacterial carbon-fixing organelle is formed by shell envelopment of preassembled cargo.

Building synthetic memory.

Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly.

An objective function exploiting suboptimal solutions in metabolic networks.

Building synthetic cellular organization.

Synthetic biology in mammalian cells: next generation research tools and therapeutics.

HITS-CLIP and Integrative Modeling Define the Rbfox Splicing-Regulatory Network Linked to Brain Development and Autism.

Programmable bacteria detect and record an environmental signal in the mammalian gut.

Integrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go.