Department of Biomedical Engineering,
Research Triangle MRSEC
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A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.
Analytical chemistry Feb, 2002 | Pubmed ID: 11838667
Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system.
Biomacromolecules Mar-Apr, 2002 | Pubmed ID: 11888323
Targeted drug delivery by thermally responsive polymers.
Advanced drug delivery reviews Sep, 2002 | Pubmed ID: 12204595
Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair.
Biomacromolecules Sep-Oct, 2002 | Pubmed ID: 12217035
Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery.
Advanced drug delivery reviews Oct, 2002 | Pubmed ID: 12384309
Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells.
Biomaterials Feb, 2003 | Pubmed ID: 12437950
Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide.
Analytical chemistry Feb, 2003 | Pubmed ID: 12622356
Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides.
Biomacromolecules May-Jun, 2003 | Pubmed ID: 12741772
Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy.
Journal of controlled release : official journal of the Controlled Release Society Aug, 2003 | Pubmed ID: 12932635
Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor.
Biomolecular engineering Jan, 2004 | Pubmed ID: 14715318
Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.
Journal of the American Chemical Society Apr, 2004 | Pubmed ID: 15080668
Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides.
Biomacromolecules May-Jun, 2004 | Pubmed ID: 15132671
Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches".
Journal of the American Chemical Society Jun, 2004 | Pubmed ID: 15186170
Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.
Analytical chemistry Sep, 2004 | Pubmed ID: 15362894
Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry.
Medical physics Oct, 2004 | Pubmed ID: 15543780
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion.
Protein science : a publication of the Protein Society Dec, 2004 | Pubmed ID: 15557268
Label free colorimetric biosensing using nanoparticles.
Journal of fluorescence Jul, 2004 | Pubmed ID: 15617380
Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide.
Biomaterials Jan, 2006 | Pubmed ID: 16023192
Self-cleavable stimulus responsive tags for protein purification without chromatography.
Journal of the American Chemical Society Aug, 2005 | Pubmed ID: 16089436
Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression.
Biomaterials Mar, 2006 | Pubmed ID: 16154191
Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays.
Journal of the American Chemical Society Oct, 2005 | Pubmed ID: 16218572
Epitope tagging for tracking elastin-like polypeptides.
Biomaterials Mar, 2006 | Pubmed ID: 16278015
Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption.
Biomacromolecules Nov-Dec, 2005 | Pubmed ID: 16283770
Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors.
Journal of controlled release : official journal of the Controlled Release Society Jan, 2006 | Pubmed ID: 16303202
Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.
Tissue engineering Nov-Dec, 2005 | Pubmed ID: 16411822
Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers.
Journal of the National Cancer Institute Mar, 2006 | Pubmed ID: 16507830
Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization.
Langmuir : the ACS journal of surfaces and colloids Apr, 2006 | Pubmed ID: 16584252
Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli.
Biotechnology progress May-Jun, 2006 | Pubmed ID: 16739944
Purification of an elastin-like fusion protein by microfiltration.
Biotechnology and bioengineering Oct, 2006 | Pubmed ID: 16767781
Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.
Journal of biomedical optics May-Jun, 2006 | Pubmed ID: 16822071
Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length.
Biomacromolecules Jul, 2006 | Pubmed ID: 16827587
Tracking the in vivo fate of recombinant polypeptides by isotopic labeling.
Journal of controlled release : official journal of the Controlled Release Society Aug, 2006 | Pubmed ID: 16904221
Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice.
Journal of controlled release : official journal of the Controlled Release Society Nov, 2006 | Pubmed ID: 16919353
A thermally responsive biopolymer for intra-articular drug delivery.
Journal of controlled release : official journal of the Controlled Release Society Oct, 2006 | Pubmed ID: 16959360
Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology.
Current opinion in chemical biology Dec, 2006 | Pubmed ID: 17055770
Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling.
Analytical biochemistry Jan, 2007 | Pubmed ID: 17084802
Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.
Langmuir : the ACS journal of surfaces and colloids Jan, 2007 | Pubmed ID: 17190524
Fabrication of gold nanowires by electric-field-induced scanning probe lithography and in situ chemical development.
Small (Weinheim an der Bergstrasse, Germany) Feb, 2007 | Pubmed ID: 17199247
Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors.
Applied optics Apr, 2007 | Pubmed ID: 17356640
Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.
Biomacromolecules May, 2007 | Pubmed ID: 17407348
Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.
Biomacromolecules May, 2007 | Pubmed ID: 17411091
Force-induced prolyl cis-trans isomerization in elastin-like polypeptides.
Journal of the American Chemical Society May, 2007 | Pubmed ID: 17469821
The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes.
Biomaterials Aug, 2007 | Pubmed ID: 17482260
Thermal cycling enhances the accumulation of a temperature-sensitive biopolymer in solid tumors.
Cancer research May, 2007 | Pubmed ID: 17483356
Plasmonic detection of a model analyte in serum by a gold nanorod sensor.
Analytical chemistry Jul, 2007 | Pubmed ID: 17567106
Toward a systems engineering approach to cancer drug delivery.
Journal of the National Cancer Institute Jul, 2007 | Pubmed ID: 17596569
Multifunctional thermally transitioning oligopeptides prepared by ring-opening metathesis polymerization.
Biomacromolecules Aug, 2007 | Pubmed ID: 17625908
Surface-initiated enzymatic polymerization of DNA.
Langmuir : the ACS journal of surfaces and colloids Nov, 2007 | Pubmed ID: 17929953
Development and characterization of a fusion protein between thermally responsive elastin-like polypeptide and interleukin-1 receptor antagonist: sustained release of a local antiinflammatory therapeutic.
Arthritis and rheumatism Nov, 2007 | Pubmed ID: 17968946
Temperature triggered self-assembly of polypeptides into multivalent spherical micelles.
Journal of the American Chemical Society Jan, 2008 | Pubmed ID: 18085778
In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair.
Biomacromolecules Jan, 2008 | Pubmed ID: 18163573
Label-free plasmonic detection of biomolecular binding by a single gold nanorod.
Analytical chemistry Feb, 2008 | Pubmed ID: 18197636
An injectable and in situ-gelling biopolymer for sustained drug release following perineural administration.
Spine Apr, 2008 | Pubmed ID: 18379401
In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model.
Tissue engineering. Part A Jul, 2008 | Pubmed ID: 18433311
Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist.
Journal of controlled release : official journal of the Controlled Release Society Aug, 2008 | Pubmed ID: 18547669
Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold film.
Nano letters Aug, 2008 | Pubmed ID: 18590340
Temperature sensitive peptides: engineering hyperthermia-directed therapeutics.
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group Sep, 2008 | Pubmed ID: 18608590
Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides.
Journal of the American Chemical Society Aug, 2008 | Pubmed ID: 18646848
Effects of Hofmeister anions on the phase transition temperature of elastin-like polypeptides.
The journal of physical chemistry. B Nov, 2008 | Pubmed ID: 18842018
Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding.
Journal of the American Chemical Society Dec, 2008 | Pubmed ID: 19055326
Fabrication of elastin-like polypeptide nanoparticles for drug delivery by electrospraying.
Biomacromolecules Jan, 2009 | Pubmed ID: 19072041
Peptide-based Biopolymers in Biomedicine and Biotechnology.
Materials science & engineering. R, Reports : a review journal Jan, 2008 | Pubmed ID: 19122836
Early metabolite levels predict long-term matrix accumulation for chondrocytes in elastin-like polypeptide biopolymer scaffolds.
Tissue engineering. Part A Aug, 2009 | Pubmed ID: 19193139
Rational selection of gold nanorod geometry for label-free plasmonic biosensors.
ACS nano Apr, 2009 | Pubmed ID: 19296619
Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins.
Protein science : a publication of the Protein Society Jul, 2009 | Pubmed ID: 19533768
In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics.
Proceedings of the National Academy of Sciences of the United States of America Sep, 2009 | Pubmed ID: 19706892
Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds.
Tissue engineering. Part A Jan, 2010 | Pubmed ID: 19754250
Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection.
Nature materials Dec, 2009 | Pubmed ID: 19898461
Hydrogen bonding of beta-turn structure is stabilized in D(2)O.
Journal of the American Chemical Society Oct, 2009 | Pubmed ID: 19919159
Elastin-like polypeptides: biomedical applications of tunable biopolymers.
Biopolymers , 2010 | Pubmed ID: 20091871
Injectable intratumoral depot of thermally responsive polypeptide-radionuclide conjugates delays tumor progression in a mouse model.
Journal of controlled release : official journal of the Controlled Release Society May, 2010 | Pubmed ID: 20117157
Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes.
Biomacromolecules Apr, 2010 | Pubmed ID: 20184309
Morphing low-affinity ligands into high-avidity nanoparticles by thermally triggered self-assembly of a genetically encoded polymer.
ACS nano Apr, 2010 | Pubmed ID: 20334355
Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.
Langmuir : the ACS journal of surfaces and colloids Jun, 2010 | Pubmed ID: 20356046
Applications of elastin-like polypeptides in tissue engineering.
Advanced drug delivery reviews Dec, 2010 | Pubmed ID: 20385185
Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation.
Biointerphases Mar, 2006 | Pubmed ID: 20408613
Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold.
Biointerphases Sep, 2008 | Pubmed ID: 20408702
Biological applications of polymer brushes. Preface.
Biointerphases Jun, 2009 | Pubmed ID: 20408712
Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale.
Biointerphases Jun, 2009 | Pubmed ID: 20408717
Drug delivery to solid tumors by elastin-like polypeptides.
Advanced drug delivery reviews Dec, 2010 | Pubmed ID: 20546809
A versatile diffractive maskless lithography for single-shot and serial microfabrication.
Optics express May, 2010 | Pubmed ID: 20589036
Stimulus-responsive macromolecules and nanoparticles for cancer drug delivery.
Nanomedicine (London, England) Jul, 2010 | Pubmed ID: 20662649
Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans.
Aquatic toxicology (Amsterdam, Netherlands) Oct, 2010 | Pubmed ID: 20708279
Leveraging nanoscale plasmonic modes to achieve reproducible enhancement of light.
Nano letters Oct, 2010 | Pubmed ID: 20804206
In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation.
Proceedings of the National Academy of Sciences of the United States of America Sep, 2010 | Pubmed ID: 20810920
Elastin-like polypeptides as a purification tag for recombinant proteins.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] Aug, 2010 | Pubmed ID: 20814933
The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.
Biomaterials Jan, 2011 | Pubmed ID: 20858564
Quantitative Model of the Phase Behavior of Recombinant pH-Responsive Elastin-Like Polypeptides.
Biomacromolecules Nov, 2010 | Pubmed ID: 20925333
Chain stiffness of elastin-like polypeptides.
Biomacromolecules Nov, 2010 | Pubmed ID: 20961120
Gold nanoparticles on polarizable surfaces as Raman scattering antennas.
ACS nano Nov, 2010 | Pubmed ID: 21038892
Spatially addressable chemoselective C-terminal ligation of an intein fusion protein from a complex mixture to a hydrazine-terminated surface.
Langmuir : the ACS journal of surfaces and colloids Feb, 2011 | Pubmed ID: 21142101
Plasmonic flow cytometry by immunolabeled nanorods.
Cytometry. Part A : the journal of the International Society for Analytical Cytology Jan, 2011 | Pubmed ID: 21182183
A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers.
Nature materials Feb, 2011 | Pubmed ID: 21258353
Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization.
Biointerphases Mar, 2011 | Pubmed ID: 21428690
Self-assembly of monodisperse oligonucleotide-elastin block copolymers into stars and compound micelles.
Chemistry (Weinheim an der Bergstrasse, Germany) May, 2011 | Pubmed ID: 21469235
Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization.
Analytical chemistry Jul, 2011 | Pubmed ID: 21604676
In vivo tumor targeting by a NGR-decorated micelle of a recombinant diblock copolypeptide.
Journal of controlled release : official journal of the Controlled Release Society Oct, 2011 | Pubmed ID: 21763734
Dual-order snapshot spectral imaging of plasmonic nanoparticles.
Applied optics Jul, 2011 | Pubmed ID: 21772408
Field-induced nanolithography for patterning of non-fouling polymer brush surfaces.
Small (Weinheim an der Bergstrasse, Germany) Nov, 2011 | Pubmed ID: 21901825
Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles.
Journal of biomedical optics Nov, 2011 | Pubmed ID: 22112108
Fusions of elastin-like polypeptides to pharmaceutical proteins.
Methods in enzymology , 2012 | Pubmed ID: 22208987
Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution.
Nano letters Apr, 2012 | Pubmed ID: 22417133
Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia.
Journal of controlled release : official journal of the Controlled Release Society May, 2012 | Pubmed ID: 22421424
Probing dynamically tunable localized surface plasmon resonances of film-coupled nanoparticles by evanescent wave excitation.
Nano letters Apr, 2012 | Pubmed ID: 22429053
Unexpected multivalent display of proteins by temperature triggered self-assembly of elastin-like polypeptide block copolymers.
Biomacromolecules May, 2012 | Pubmed ID: 22515311
Protein polymer hydrogels by in situ, rapid and reversible self-gelation.
Biomaterials Jul, 2012 | Pubmed ID: 22538198
Digital switching of local arginine density in a genetically encoded self-assembled polypeptide nanoparticle controls cellular uptake.
Nano letters Jun, 2012 | Pubmed ID: 22625178
Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materials.
Aquatic toxicology (Amsterdam, Netherlands) Sep, 2012 | Pubmed ID: 22634717
Rheological properties of cysteine-containing elastin-like polypeptide solutions and hydrogels.
Biomacromolecules Aug, 2012 | Pubmed ID: 22789001
Chemistry. Nanomaterials for drug delivery.
Science (New York, N.Y.) Jul, 2012 | Pubmed ID: 22822138
Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors.
Cancer research Nov, 2012 | Pubmed ID: 22952218
Plasmon ruler with angstrom length resolution.
ACS nano Oct, 2012 | Pubmed ID: 22966857
Harnessing the power of cell-penetrating peptides: activatable carriers for targeting systemic delivery of cancer therapeutics and imaging agents.
Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology Jan-Feb, 2013 | Pubmed ID: 22977001
Fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures by an irradiation-promoted exchange reaction.
Angewandte Chemie (International ed. in English) Oct, 2012 | Pubmed ID: 22987725
Effect of detergents on the thermal behavior of elastin-like polypeptides.
Biopolymers Jan, 2013 | Pubmed ID: 23097230
Brachytherapy using injectable seeds that are self-assembled from genetically encoded polypeptides in situ.
Cancer research Nov, 2012 | Pubmed ID: 23155121
Direct fluorescence detection of RNA on microarrays by surface-initiated enzymatic polymerization.
Analytical chemistry Jan, 2013 | Pubmed ID: 23194025
Controlled-reflectance surfaces with film-coupled colloidal nanoantennas.
Nature Dec, 2012 | Pubmed ID: 23222613
Self-assembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation.
Angewandte Chemie (International ed. in English) Feb, 2013 | Pubmed ID: 23280697
Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control.
Proceedings of the National Academy of Sciences of the United States of America Feb, 2013 | Pubmed ID: 23359691
Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.
Angewandte Chemie (International ed. in English) Mar, 2013 | Pubmed ID: 23424160
Predicting transition temperatures of elastin-like polypeptide fusion proteins.
Biomacromolecules May, 2013 | Pubmed ID: 23565607
Calcium binding peptide motifs from calmodulin confer divalent ion selectivity to elastin-like polypeptides.
Biomacromolecules Jul, 2013 | Pubmed ID: 23705904
A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures.
Biomacromolecules Aug, 2013 | Pubmed ID: 23808597
Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers.
Journal of the American Chemical Society Jul, 2013 | Pubmed ID: 23822733
A genetically engineered thermally responsive sustained release curcumin depot to treat neuroinflammation.
Journal of controlled release : official journal of the Controlled Release Society Oct, 2013 | Pubmed ID: 23830979
Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.
Macromolecular rapid communications Aug, 2013 | Pubmed ID: 23836349
Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization.
The journal of physical chemistry. B Aug, 2013 | Pubmed ID: 23899324
Actively targeting solid tumours with thermoresponsive drug delivery systems that respond to mild hyperthermia.
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group Sep, 2013 | Pubmed ID: 23924317
NANOSCALE SELF-ASSEMBLY FOR DELIVERY OF THERAPEUTICS AND IMAGING AGENTS.
Technology and innovation Jan, 2011 | Pubmed ID: 24077873
Plasmonic waveguide modes of film-coupled metallic nanocubes.
Nano letters Dec, 2013 | Pubmed ID: 24199752
"Smart" DNA interfaces.
Chemical Society reviews Mar, 2014 | Pubmed ID: 24352168
Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles.
Advanced materials (Deerfield Beach, Fla.) May, 2014 | Pubmed ID: 24497034
Controlled apoptosis by a thermally toggled nanoscale amplifier of cellular uptake.
Nano letters Apr, 2014 | Pubmed ID: 24611762
Rational design of "heat seeking" drug loaded polypeptide nanoparticles that thermally target solid tumors.
Nano letters May, 2014 | Pubmed ID: 24738626
Simple Assay for Proteases Based on Aggregation of Stimulus-Responsive Polypeptides.
Analytical chemistry May, 2014 | Pubmed ID: 24832919
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