Ashutosh Chilkoti

A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.

Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system.

Targeted drug delivery by thermally responsive polymers.

Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair.

Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery.

Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells.

Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide.

Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides.

Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy.

Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor.

Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.

Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides.

Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches".

Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.

Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry.

Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion.

Label free colorimetric biosensing using nanoparticles.

Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide.

Self-cleavable stimulus responsive tags for protein purification without chromatography.

Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression.

Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays.

Epitope tagging for tracking elastin-like polypeptides.

Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption.

Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors.

Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.

Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers.

Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization.

Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli.

Purification of an elastin-like fusion protein by microfiltration.

Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.

Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length.

Tracking the in vivo fate of recombinant polypeptides by isotopic labeling.

Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice.

A thermally responsive biopolymer for intra-articular drug delivery.

Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology.

Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling.

Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.

Fabrication of gold nanowires by electric-field-induced scanning probe lithography and in situ chemical development.

Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors.

Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.

Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.

Force-induced prolyl cis-trans isomerization in elastin-like polypeptides.

The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes.

Thermal cycling enhances the accumulation of a temperature-sensitive biopolymer in solid tumors.

Plasmonic detection of a model analyte in serum by a gold nanorod sensor.

Toward a systems engineering approach to cancer drug delivery.

Multifunctional thermally transitioning oligopeptides prepared by ring-opening metathesis polymerization.

Surface-initiated enzymatic polymerization of DNA.

Development and characterization of a fusion protein between thermally responsive elastin-like polypeptide and interleukin-1 receptor antagonist: sustained release of a local antiinflammatory therapeutic.

Temperature triggered self-assembly of polypeptides into multivalent spherical micelles.

In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair.

Label-free plasmonic detection of biomolecular binding by a single gold nanorod.

An injectable and in situ-gelling biopolymer for sustained drug release following perineural administration.

In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model.

Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist.

Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold film.

Temperature sensitive peptides: engineering hyperthermia-directed therapeutics.

Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides.

Effects of Hofmeister anions on the phase transition temperature of elastin-like polypeptides.

Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding.

Fabrication of elastin-like polypeptide nanoparticles for drug delivery by electrospraying.

Peptide-based Biopolymers in Biomedicine and Biotechnology.

Early metabolite levels predict long-term matrix accumulation for chondrocytes in elastin-like polypeptide biopolymer scaffolds.

Rational selection of gold nanorod geometry for label-free plasmonic biosensors.

Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins.

In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics.

Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds.

Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection.

Hydrogen bonding of beta-turn structure is stabilized in D(2)O.

Elastin-like polypeptides: biomedical applications of tunable biopolymers.

Injectable intratumoral depot of thermally responsive polypeptide-radionuclide conjugates delays tumor progression in a mouse model.

Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes.

Morphing low-affinity ligands into high-avidity nanoparticles by thermally triggered self-assembly of a genetically encoded polymer.

Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.

Applications of elastin-like polypeptides in tissue engineering.

Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation.

Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold.

Biological applications of polymer brushes. Preface.

Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale.

Drug delivery to solid tumors by elastin-like polypeptides.

A versatile diffractive maskless lithography for single-shot and serial microfabrication.

Stimulus-responsive macromolecules and nanoparticles for cancer drug delivery.

Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans.

Leveraging nanoscale plasmonic modes to achieve reproducible enhancement of light.

In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation.

Elastin-like polypeptides as a purification tag for recombinant proteins.

The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.

Quantitative Model of the Phase Behavior of Recombinant pH-Responsive Elastin-Like Polypeptides.

Chain stiffness of elastin-like polypeptides.

Gold nanoparticles on polarizable surfaces as Raman scattering antennas.

Spatially addressable chemoselective C-terminal ligation of an intein fusion protein from a complex mixture to a hydrazine-terminated surface.

Plasmonic flow cytometry by immunolabeled nanorods.

A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers.

Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization.

Self-assembly of monodisperse oligonucleotide-elastin block copolymers into stars and compound micelles.

Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization.

In vivo tumor targeting by a NGR-decorated micelle of a recombinant diblock copolypeptide.

Dual-order snapshot spectral imaging of plasmonic nanoparticles.

Field-induced nanolithography for patterning of non-fouling polymer brush surfaces.

Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles.

Fusions of elastin-like polypeptides to pharmaceutical proteins.

Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution.

Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia.

Probing dynamically tunable localized surface plasmon resonances of film-coupled nanoparticles by evanescent wave excitation.

Unexpected multivalent display of proteins by temperature triggered self-assembly of elastin-like polypeptide block copolymers.

Protein polymer hydrogels by in situ, rapid and reversible self-gelation.

Digital switching of local arginine density in a genetically encoded self-assembled polypeptide nanoparticle controls cellular uptake.

Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materials.

Rheological properties of cysteine-containing elastin-like polypeptide solutions and hydrogels.

Chemistry. Nanomaterials for drug delivery.

Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors.

Plasmon ruler with angstrom length resolution.

Harnessing the power of cell-penetrating peptides: activatable carriers for targeting systemic delivery of cancer therapeutics and imaging agents.

Fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures by an irradiation-promoted exchange reaction.

Effect of detergents on the thermal behavior of elastin-like polypeptides.

Brachytherapy using injectable seeds that are self-assembled from genetically encoded polypeptides in situ.

Direct fluorescence detection of RNA on microarrays by surface-initiated enzymatic polymerization.

Controlled-reflectance surfaces with film-coupled colloidal nanoantennas.

Self-assembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation.

Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control.

Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.

Predicting transition temperatures of elastin-like polypeptide fusion proteins.

Calcium binding peptide motifs from calmodulin confer divalent ion selectivity to elastin-like polypeptides.

A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures.

Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers.

A genetically engineered thermally responsive sustained release curcumin depot to treat neuroinflammation.

Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.

Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization.

Actively targeting solid tumours with thermoresponsive drug delivery systems that respond to mild hyperthermia.

NANOSCALE SELF-ASSEMBLY FOR DELIVERY OF THERAPEUTICS AND IMAGING AGENTS.

Plasmonic waveguide modes of film-coupled metallic nanocubes.

"Smart" DNA interfaces.

Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles.

Controlled apoptosis by a thermally toggled nanoscale amplifier of cellular uptake.

Rational design of "heat seeking" drug loaded polypeptide nanoparticles that thermally target solid tumors.

Simple Assay for Proteases Based on Aggregation of Stimulus-Responsive Polypeptides.