Laboratory for Optical and Computational Instrumentation,
Department of Biomedical Engineering,
Morgridge Institute for Research,
Center for Quantitative Cell Imaging,
Departments of Medical Physics and Biomedical Engineering,
Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging
Kevin Eliceiri received his undergraduate and graduate training in Microbiology and Biotechnology at the University of Wisconsin in Madison. He worked in Professor John White's laboratory developing imaging approaches for the model nematode C. elegans. He received further post-graduate training at the National Integrated Microscopy Resource (Madison, Wisconsin) in the area of computer science and microscopy. Since 2000 he has lead investigator of the Laboratory for Optical and Computational Instrumentation (LOCI) at the University of Wisconsin at Madison. He is currently RRF Walter H. Helmerich Professor in Medical Physics and Biomedical Engineering, a investigator in the Morgridge Institute for Research, and a Principal Investigator in the Center for Quantitative Cell Imaging at the University of Wisconsin in Madison Graduate School. His current research focuses on the development of novel optical imaging methods for investigating cell signalling and cancer progression, and the development of software for multidimensional image analysis.
Analysis of multidimensional biological image data.
BioTechniques Dec, 2002 | Pubmed ID: 12503311
Analysis of histology specimens using lifetime multiphoton microscopy.
Journal of biomedical optics Jul, 2003 | Pubmed ID: 12880342
VisBio: a computational tool for visualization of multidimensional biological image data.
Traffic (Copenhagen, Denmark) Jun, 2004 | Pubmed ID: 15117315
Molecular expressions: exploring the world of optics and microscopy. http://microscopy.fsu.edu.
Biology of the cell / under the auspices of the European Cell Biology Organization Aug, 2004 | Pubmed ID: 15325069
Simultaneous two-photon spectral and lifetime fluorescence microscopy.
Applied optics Sep, 2004 | Pubmed ID: 15473237
Tools for visualizing multidimensional images from living specimens.
Photochemistry and photobiology Sep-Oct, 2005 | Pubmed ID: 15807634
Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH.
Cancer research Oct, 2005 | Pubmed ID: 16204046
Applications of combined spectral lifetime microscopy for biology.
BioTechniques Sep, 2006 | Pubmed ID: 16989084
BioClips of symmetric and asymmetric cell division.
Biology of the cell / under the auspices of the European Cell Biology Organization May, 2007 | Pubmed ID: 17437407
WormClassroom.org: an inquiry-rich educational web portal for research resources of Caenorhabditis elegans.
CBE life sciences education , 2007 | Pubmed ID: 17548872
Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase.
Development (Cambridge, England) Nov, 2007 | Pubmed ID: 17913784
Student learning of early embryonic development via the utilization of research resources from the nematode Caenorhabditis elegans.
CBE life sciences education , 2008 | Pubmed ID: 18316809
Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities.
The Review of scientific instruments Jan, 2003 | Pubmed ID: 18607511
Bimolecular fluorescence complementation analysis of eukaryotic fusion products.
Biology of the cell / under the auspices of the European Cell Biology Organization Sep, 2010 | Pubmed ID: 20590528
Unifying Biological Image Formats with HDF5.
Communications of the ACM Oct, 2009 | Pubmed ID: 21218176
Fast localized wavefront correction using area-mapped phase-shift interferometry.
Optics letters Aug, 2011 | Pubmed ID: 21808349
Structural changes in mixed Col I/Col V collagen gels probed by SHG microscopy: implications for probing stromal alterations in human breast cancer.
Biomedical optics express Aug, 2011 | Pubmed ID: 21833367
A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart.
Regenerative medicine Sep, 2011 | Pubmed ID: 21916593
IMAGE RECONSTRUCTION OF MULTIPHOTON MICROSCOPY DATA.
Proceedings / IEEE International Symposium on Biomedical Imaging: from nano to macro. IEEE International Symposium on Biomedical Imaging Jun, 2009 | Pubmed ID: 22158826
Imaging cardiac extracellular matrices: a blueprint for regeneration.
Trends in biotechnology Dec, 2011 | Pubmed ID: 22209562
Cell death, non-invasively assessed by intrinsic fluorescence intensity of NADH, is a predictive indicator of functional differentiation of embryonic stem cells.
Biology of the cell / under the auspices of the European Cell Biology Organization Jun, 2012 | Pubmed ID: 22304470
Biological imaging software tools.
Nature methods Jul, 2012 | Pubmed ID: 22743775
Mesenchymal stem cell interactions with 3D ECM modules fabricated via multiphoton excited photochemistry.
Biomacromolecules Sep, 2012 | Pubmed ID: 22876971
Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient.
Biomedical optics express Nov, 2012 | Pubmed ID: 23162710
Spatial and temporal analysis of extracellular matrix proteins in the developing murine heart: a blueprint for regeneration.
Tissue engineering. Part A May, 2013 | Pubmed ID: 23273220
Nonlinear optical microscopy and ultrasound imaging of human cervical structure.
Journal of biomedical optics Mar, 2013 | Pubmed ID: 23412434
RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis.
Molecular biology of the cell Jun, 2013 | Pubmed ID: 23552690
OpenSPIM: an open-access light-sheet microscopy platform.
Nature methods Jul, 2013 | Pubmed ID: 23749304
ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments.
Biomacromolecules Sep, 2013 | Pubmed ID: 23875943
Simultaneous determination of the second-harmonic generation emission directionality and reduced scattering coefficient from three-dimensional imaging of thick tissues.
Journal of biomedical optics Nov, 2013 | Pubmed ID: 24220726
Association of cellular and molecular responses in the rat mammary gland to 17β-estradiol with susceptibility to mammary cancer.
BMC cancer , 2013 | Pubmed ID: 24304664
Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues.
Optics letters Apr, 2014 | Pubmed ID: 24686633
A microfluidic coculture and multiphoton FAD analysis assay provides insight into the influence of the bone microenvironment on prostate cancer cells.
Integrative biology : quantitative biosciences from nano to macro Jun, 2014 | Pubmed ID: 24791272
The effect of micro-ECoG substrate footprint on the meningeal tissue response.
Journal of neural engineering Aug, 2014 | Pubmed ID: 24941335
Cooperativity among Rev-associated nuclear export signals regulates HIV-1 gene expression and is a determinant of virus species tropism.
Journal of virology Dec, 2014 | Pubmed ID: 25275125
A three-dimensional computational model of collagen network mechanics.
PloS one , 2014 | Pubmed ID: 25386649
3D collagen alignment limits protrusions to enhance breast cancer cell persistence.
Biophysical journal Dec, 2014 | Pubmed ID: 25468334
Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells.
PloS one , 2015 | Pubmed ID: 25607819
The sweet spot: FDG and other 2-carbon glucose analogs for multi-modal metabolic imaging of tumor metabolism.
American journal of nuclear medicine and molecular imaging , 2015 | Pubmed ID: 25625022
Multi-functional self-fluorescent unimolecular micelles for tumor-targeted drug delivery and bioimaging.
Biomaterials Apr, 2015 | Pubmed ID: 25682159
Endogenous Optical Signals Reveal Changes of Elastin and Collagen Organization During Differentiation of Mouse Embryonic Stem Cells.
Tissue engineering. Part C, Methods Oct, 2015 | Pubmed ID: 25923353
Matrix metalloproteinase 9 modulates collagen matrices and wound repair.
Development (Cambridge, England) Jun, 2015 | Pubmed ID: 26015541
Multi-view second-harmonic generation imaging of mouse tail tendon via reflective micro-prisms.
Optics letters Jul, 2015 | Pubmed ID: 26125402
Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism.
The Plant cell Sep, 2015 | Pubmed ID: 26342015
Advanced Materials for Neural Surface Electrodes.
Current opinion in solid state & materials science Dec, 2014 | Pubmed ID: 26392802
Expression of the Drosophila homeobox gene, Distal-less, supports an ancestral role in neural development.
Developmental dynamics : an official publication of the American Association of Anatomists Jan, 2016 | Pubmed ID: 26472170
Validation of an arterial constitutive model accounting for collagen content and crosslinking.
Acta biomaterialia Feb, 2016 | Pubmed ID: 26654765
Charcot-Marie-Tooth 2b associated Rab7 mutations cause axon growth and guidance defects during vertebrate sensory neuron development.
Neural development , 2016 | Pubmed ID: 26791407
Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors.
Cell stem cell Mar, 2016 | Pubmed ID: 26877223
Blue Light Modulates Murine Microglial Gene Expression in the Absence of Optogenetic Protein Expression.
Scientific reports , 2016 | Pubmed ID: 26883795
Advanced Intestinal Cancers often Maintain a Multi-Ancestral Architecture.
PloS one , 2016 | Pubmed ID: 26919712
Radiation Promptly Alters Cancer Live Cell Metabolic Fluxes: An In Vitro Demonstration.
Radiation research Apr, 2016 | Pubmed ID: 27128739
Membrane dynamics during cellular wound repair.
Molecular biology of the cell Jul, 2016 | Pubmed ID: 27226483
Second harmonic generation microscopy in zebrafish.
Methods in cell biology , 2016 | Pubmed ID: 27263408
Pancreatic β-Cells From Mice Offset Age-Associated Mitochondrial Deficiency With Reduced KATP Channel Activity.
Diabetes Sep, 2016 | Pubmed ID: 27284112
Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells.
Oncotarget Jul, 2016 | Pubmed ID: 27344177
Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 09, 2016 | Pubmed ID: 27449741
Using fluorescence lifetime microscopy to study the subcellular localization of anthocyanins.
The Plant journal : for cell and molecular biology Dec, 2016 | Pubmed ID: 27500780
Development of a novel linearly-filled Derenzo microPET phantom.
American journal of nuclear medicine and molecular imaging , 2016 | Pubmed ID: 27508106
Administration of Non-Torsadogenic human Ether-à-go-go-Related Gene Inhibitors Is Associated with Better Survival for High hERG-Expressing Glioblastoma Patients.
Clinical cancer research : an official journal of the American Association for Cancer Research Jan, 2017 | Pubmed ID: 27635088
TrackMate: An open and extensible platform for single-particle tracking.
Methods (San Diego, Calif.) Feb, 2017 | Pubmed ID: 27713081
3D texture analysis for classification of second harmonic generation images of human ovarian cancer.
Scientific reports Oct, 2016 | Pubmed ID: 27767180
ImageJ-MATLAB: a bidirectional framework for scientific image analysis interoperability.
Bioinformatics (Oxford, England) Feb, 2017 | Pubmed ID: 27797782
Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging.
Molecular biology of the cell Feb, 2017 | Pubmed ID: 27903772
SCIFIO: an extensible framework to support scientific image formats.
BMC bioinformatics Dec, 2016 | Pubmed ID: 27927161
Beyond the margins: real-time detection of cancer using targeted fluorophores.
Nature reviews. Clinical oncology Jun, 2017 | Pubmed ID: 28094261
Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells.
Breast cancer research : BCR Jan, 2017 | Pubmed ID: 28103936
Aging and caloric restriction impact adipose tissue, adiponectin, and circulating lipids.
Aging cell Jun, 2017 | Pubmed ID: 28156058
Stromal alterations in ovarian cancers via wavelength dependent Second Harmonic Generation microscopy and optical scattering.
BMC cancer Feb, 2017 | Pubmed ID: 28166758
Complex and Noncentrosymmetric Stacking of Layered Metal Dichalcogenide Materials Created by Screw Dislocations.
Journal of the American Chemical Society Mar, 2017 | Pubmed ID: 28177621
Enriching Islet Phospholipids With Eicosapentaenoic Acid Reduces Prostaglandin E2 Signaling and Enhances Diabetic β-Cell Function.
Diabetes Jun, 2017 | Pubmed ID: 28193789
Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 08, 2017 | Pubmed ID: 28692327
Methods for Quantifying Fibrillar Collagen Alignment.
Methods in molecular biology (Clifton, N.J.) , 2017 | Pubmed ID: 28836218
Neuroendocrine Tumor-Targeted Upconversion Nanoparticle-Based Micelles for Simultaneous NIR-Controlled Combination Chemotherapy and Photodynamic Therapy, and Fluorescence Imaging.
Advanced functional materials Feb, 2017 | Pubmed ID: 28989337
FunImageJ: a Lisp framework for scientific image processing.
Bioinformatics (Oxford, England) 03, 2018 | Pubmed ID: 29106446
Collagen Alignment as a Predictor of Recurrence after Ductal Carcinoma .
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 02, 2018 | Pubmed ID: 29141852
ImageJ2: ImageJ for the next generation of scientific image data.
BMC bioinformatics Nov, 2017 | Pubmed ID: 29187165
Damage-induced reactive oxygen species regulate and dynamic collagen-based projections to mediate wound repair.
eLife 01, 2018 | Pubmed ID: 29336778
Review of quantitative multiscale imaging of breast cancer.
Journal of medical imaging (Bellingham, Wash.) Jan, 2018 | Pubmed ID: 29392158
The Presence of Cyclooxygenase 2, Tumor-Associated Macrophages, and Collagen Alignment as Prognostic Markers for Invasive Breast Carcinoma Patients.
The American journal of pathology 03, 2018 | Pubmed ID: 29429545
Quantification of Collagen Organization after Nerve Repair.
Plastic and reconstructive surgery. Global open Dec, 2017 | Pubmed ID: 29632766
GSK3β Regulates Brain Energy Metabolism.
Cell reports 05, 2018 | Pubmed ID: 29768193
Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM).
Methods in molecular biology (Clifton, N.J.) , 2018 | Pubmed ID: 29916076
Void spot assay procedural optimization and software for rapid and objective quantification of rodent voiding function, including overlapping urine spots.
American journal of physiology. Renal physiology Jul, 2018 | Pubmed ID: 29972322
Fabrication approaches for the creation of physical models from microscopy data.
3D printing in medicine , 2017 | Pubmed ID: 30050979
Chemically Derived Kirigami of WSe.
Journal of the American Chemical Society 09, 2018 | Pubmed ID: 30081633
Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma.
Scientific reports 08, 2018 | Pubmed ID: 30154546
Imaging the Cardiac Extracellular Matrix.
Advances in experimental medicine and biology , 2018 | Pubmed ID: 30238364
Convolutional neural networks for whole slide image superresolution.
Biomedical optics express Nov, 2018 | Pubmed ID: 30460134
Quantitative second harmonic generation imaging of leporine, canine, and porcine vocal fold collagen.
The Laryngoscope 11, 2019 | Pubmed ID: 30628080
Transglutaminase-2 Mediates the Biomechanical Properties of the Colorectal Cancer Tissue Microenvironment that Contribute to Disease Progression.
Cancers May, 2019 | Pubmed ID: 31117256
Collagen organization of renal cell carcinoma differs between low and high grade tumors.
BMC cancer May, 2019 | Pubmed ID: 31122202
Distinct inflammatory and wound healing responses to complex caudal fin injuries of larval zebrafish.
eLife 07, 2019 | Pubmed ID: 31259685
PGC-1a integrates a metabolism and growth network linked to caloric restriction.
Aging cell 10, 2019 | Pubmed ID: 31267675
Quantitative Histopathology of Stained Tissues using Color Spatial Light Interference Microscopy (cSLIM).
Scientific reports 10, 2019 | Pubmed ID: 31604963
Optimization of interstrand interactions enables burn detection with a collagen-mimetic peptide.
Organic & biomolecular chemistry 11, 2019 | Pubmed ID: 31720665
Molecular and Functional Networks Linked to Sarcopenia Prevention by Caloric Restriction in Rhesus Monkeys.
Cell systems 02, 2020 | Pubmed ID: 31982367
Intensity-based registration of bright-field and second-harmonic generation images of histopathology tissue sections.
Biomedical optics express Jan, 2020 | Pubmed ID: 32010507
A syringe adapter for reduced muscular strain and fatigue.
Applied ergonomics May, 2020 | Pubmed ID: 32174349
TRIM32 cooperates with glycolytic enzymes to promote cell growth.
eLife Mar, 2020 | Pubmed ID: 32223900
Citrullination regulates wound responses and tissue regeneration in zebrafish.
The Journal of cell biology Apr, 2020 | Pubmed ID: 32328635
3-D-Printed Registration Phantom for Combined Ultrasound and Optical Imaging of Biological Tissues.
Ultrasound in medicine & biology Jul, 2020 | Pubmed ID: 32340797
Platform for quantitative multiscale imaging of tissue composition.
Biomedical optics express Apr, 2020 | Pubmed ID: 32341858
A semi-automated machine-learning based workflow for ellipsoid zone analysis in eyes with macular edema: SCORE2 pilot study.
PloS one , 2020 | Pubmed ID: 32353052
Fibrillar Collagen Quantification With Curvelet Transform Based Computational Methods.
Frontiers in bioengineering and biotechnology , 2020 | Pubmed ID: 32373594
Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Nov, 2020 | Pubmed ID: 32686215
Optical imaging of collagen fiber damage to assess thermally injured human skin.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Nov, 2020 | Pubmed ID: 32715561
Non-disruptive collagen characterization in clinical histopathology using cross-modality image synthesis.
Communications biology Jul, 2020 | Pubmed ID: 32737412
Microglia activation visualization via fluorescence lifetime imaging microscopy of intrinsically fluorescent metabolic cofactors.
Neurophotonics Jul, 2020 | Pubmed ID: 32821772
Integration of the ImageJ Ecosystem in the KNIME Analytics Platform.
Frontiers in computer science Mar, 2020 | Pubmed ID: 32905440
Machine Learning Methods for Fluorescence Lifetime Imaging (FLIM) Based Label-Free Detection of Microglia.
Frontiers in neuroscience , 2020 | Pubmed ID: 33013309
Collagen Organization in Relation to Ductal Carcinoma Pathology and Outcomes.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology Oct, 2020 | Pubmed ID: 33082201
Microstructure and resident cell-types of the feline optic nerve head resemble that of humans.
Experimental eye research Oct, 2020 | Pubmed ID: 33091431
Challenges of conducting quantitative ultrasound with a multimodal optical imaging system.
Physics in medicine and biology Nov, 2020 | Pubmed ID: 33171448
Brian Burkel1,2,
Brett A. Morris1,
Suzanne M. Ponik1,
Kristin M. Riching1,
Kevin W. Eliceiri2,3,4,
Patricia J. Keely1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison,
2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison,
3Department of Biomedical Engineering, University of Wisconsin-Madison,
4Morgridge Institute for Research, University of Wisconsin-Madison,
5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison
Kayla Huemer*,1,2,
Jayne M. Squirrell*,3,
Robert Swader2,
Kirsten Pelkey2,
Danny C. LeBert4,
Anna Huttenlocher5,6,
Kevin W. Eliceiri1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison,
2Morgridge Institute for Research, University of Wisconsin-Madison,
3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison,
4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison,
5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison,
6Department of Pediatrics, University of Wisconsin-Madison
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