Walter Keller

The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins.

Mutants of the major ryegrass pollen allergen, Lol p 5, with reduced IgE-binding capacity: candidates for grass pollen-specific immunotherapy.

Functional analysis of yeast snoRNA and snRNA 3'-end formation mediated by uncoupling of cleavage and polyadenylation.

Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy.

tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli.

Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.

Combination vaccines for the treatment of grass pollen allergy consisting of genetically engineered hybrid molecules with increased immunogenicity.

Gene regulation: reviving the message.

The cross-reactive calcium-binding pollen allergen, Phl p 7, reveals a novel dimer assembly.

Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen.

A role for SSU72 in balancing RNA polymerase II transcription elongation and termination.

RNA editing by adenosine deaminases generates RNA and protein diversity.

Three-dimensional structure of the panallergen Phl p 7.

X-filtering for a range of coupling constants: application to the detection of intermolecular NOEs.

Molecular characterization of recombinant T1, a non-allergenic periwinkle (Catharanthus roseus) protein, with sequence similarity to the Bet v 1 plant allergen family.

Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination.

Formation of disulfide bonds and homodimers of the major cat allergen Fel d 1 equivalent to the natural allergen by expression in Escherichia coli.

The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation.

Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.

Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.

Circular dichroism analysis of allergens.

Ribavirin/interferon-alpha sequential treatment of recurrent hepatitis C after liver transplantation.

Generation of an allergy vaccine by disruption of the three-dimensional structure of the cross-reactive calcium-binding allergen, Phl p 7.

Molecular characterization of polygalacturonases as grass pollen-specific marker allergens: expulsion from pollen via submicronic respirable particles.

Sequence motifs that distinguish ATP(CTP):tRNA nucleotidyl transferases from eubacterial poly(A) polymerases.

Functions for S. cerevisiae Swd2p in 3' end formation of specific mRNAs and snoRNAs and global histone 3 lysine 4 methylation.

Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization.

Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase.

TraA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution.

A new yeast poly(A) polymerase complex involved in RNA quality control.

A hybrid molecule resembling the epitope spectrum of grass pollen for allergy vaccination.

WW domains provide a platform for the assembly of multiprotein networks.

Dimerization of the major birch pollen allergen Bet v 1 is important for its in vivo IgE-cross-linking potential in mice.

Bacterial fermentation of recombinant major wasp allergen Antigen 5 using oxygen limiting growth conditions improves yield and quality of inclusion bodies.

Crystallization and preliminary structure determination of the plant food allergen Pru av 2.

The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501.

Cooking birch pollen-related food: divergent consequences for IgE- and T cell-mediated reactivity in vitro and in vivo.

Direct interactions between subunits of CPSF and the U2 snRNP contribute to the coupling of pre-mRNA 3' end processing and splicing.

Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA.

An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries.

Bacterially expressed and optimized recombinant Phl p 1 is immunobiochemically equivalent to natural Phl p 1.

Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals.

A recombinant hypoallergenic parvalbumin mutant for immunotherapy of IgE-mediated fish allergy.

Clinical clarifications requested.

Genetic engineering of the major timothy grass pollen allergen, Phl p 6, to reduce allergenic activity and preserve immunogenicity.

The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding.

RNA-specific ribonucleotidyl transferases.

Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2.

A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin.

Determinants of substrate specificity in RNA-dependent nucleotidyl transferases.

Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens.

Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering.

Crystal structure of the 25 kDa subunit of human cleavage factor Im.

Characterization of folded recombinant Der p 5, a potential diagnostic marker allergen for house dust mite allergy.

The structure and binding behavior of the bacterial cell surface layer protein SbsC.

Disruption of allergenic activity of the major grass pollen allergen Phl p 2 by reassembly as a mosaic protein.

The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis.

Cloning, expression, and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen.

The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions.

Distinct roles of non-canonical poly(A) polymerases in RNA metabolism.

Towards the structure of the C-terminal part of the S-layer protein SbsC.

Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A.

Visualization of clustered IgE epitopes on alpha-lactalbumin.

Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I.

Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly.

Biophysical characterization of recombinant HIV-1 subtype C virus infectivity factor.

Ground vegetation monitoring in Swiss forests: comparison of survey methods and implications for trend assessments.

Altered IgE epitope presentation: A model for hypoallergenic activity revealed for Bet v 1 trimer.

Antiparasitic compounds from Cupania cinerea with activities against Plasmodium falciparum and Trypanosoma brucei rhodesiense.

PAPD5, a noncanonical poly(A) polymerase with an unusual RNA-binding motif.

Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity.

The structure of bacterial S-layer proteins.

Misdirected antibody responses against an N-terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections.

Effect of protamine on the solubility and deamidation of human growth hormone.

Hypoallergenic mutants of the Timothy grass pollen allergen Phl p 5 generated by proline mutations.

Genome-wide analysis of pre-mRNA 3' end processing reveals a decisive role of human cleavage factor I in the regulation of 3' UTR length.

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system.

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

Cleavage factor Im is a key regulator of 3' UTR length.

The 2.5 Å structure of the enterococcus conjugation protein TraM resembles VirB8 type IV secretion proteins.

Crystallization of domains involved in self-assembly of the S-layer protein SbsC.

Different modes of IgE binding to CD23 revealed with major birch allergen, Bet v 1-specific monoclonal IgE.

Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501.

A nonallergenic birch pollen allergy vaccine consisting of hepatitis PreS-fused Bet v 1 peptides focuses blocking IgG toward IgE epitopes and shifts immune responses to a tolerogenic and Th1 phenotype.

Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets.

Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4.

Backbone resonance assignment of Alt a 1, a unique β-barrel protein and the major allergen of Alternaria alternata.

Characterization of mutants of a highly cross-reactive calcium-binding protein from Brassica pollen for allergen-specific immunotherapy.

Structure of allergens and structure based epitope predictions.

TraG encoded by the pIP501 type IV secretion system is a two-domain peptidoglycan-degrading enzyme essential for conjugative transfer.

Crystal structure of human poly(A) polymerase gamma reveals a conserved catalytic core for canonical poly(A) polymerases.

Conjugative type IV secretion systems in Gram-positive bacteria.

Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5.

Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors.

The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold.

Structure of the double-stranded DNA-binding type IV secretion protein TraN from Enterococcus.

Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33.

Biochemical, biophysical and IgE-epitope characterization of the wheat food allergen, Tri a 37.

Global 3' UTR shortening has a limited effect on protein abundance in proliferating T cells.

Comparison of the specificities of IgG, IgG-subclass, IgA and IgM reactivities in African and European HIV-infected individuals with an HIV-1 clade C proteome-based array.

Reflections on the RNA world.

Conjugation in Gram-Positive Bacteria.

Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion.

Tracking morphologies at the nanoscale: self-assembly of an amphiphilic designer peptide into a double helix superstructure.

1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501.

IgE epitope proximity determines immune complex shape and effector cell activation capacity.

VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis.

Critical and direct involvement of the CD23 stalk region in IgE binding.

A comprehensive analysis of 3' end sequencing data sets reveals novel polyadenylation signals and the repressive role of heterogeneous ribonucleoprotein C on cleavage and polyadenylation.

Mitochondrial lipids in neurodegeneration.

Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen.

Conjugative type IV secretion in Gram-positive pathogens: TraG, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein TraM.

The Coordinated Action of Calcineurin and Cathepsin D Protects Against α-Synuclein Toxicity.

Targeting Type IV Secretion System Proteins to Combat Multidrug-Resistant Gram-positive Pathogens.

Clustering of conformational IgE epitopes on the major dog allergen Can f 1.