Department of Agronomy,
Crop Bioengineering Center
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Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.
Plant physiology May, 2002 | Pubmed ID: 12011333
A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT).
Transgenic research Oct, 2002 | Pubmed ID: 12437079
Localization of a bacterial protein in starch granules of transgenic maize kernels.
Proceedings of the National Academy of Sciences of the United States of America Sep, 2003 | Pubmed ID: 12960364
Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize.
Proceedings of the National Academy of Sciences of the United States of America Mar, 2004 | Pubmed ID: 14960727
Expression of the Nicotiana protein kinase (NPK1) enhanced drought tolerance in transgenic maize.
Journal of experimental botany May, 2004 | Pubmed ID: 15073214
Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation.
Plant cell reports Mar, 2006 | Pubmed ID: 16249869
Analysis of immune response in young and aged mice vaccinated with corn-derived antigen against Escherichia coli heat-labile enterotoxin.
Molecular biotechnology Jan, 2006 | Pubmed ID: 16382180
Improved Agrobacterium-mediated transformation of three maize inbred lines using MS salts.
Plant cell reports Oct, 2006 | Pubmed ID: 16710703
Gene expression patterns during somatic embryo development and germination in maize Hi II callus cultures.
Plant molecular biology Sep, 2006 | Pubmed ID: 16845483
Maize (Zea mays L.).
Methods in molecular biology (Clifton, N.J.) , 2006 | Pubmed ID: 16988344
Genetic engineering approaches to improve bioethanol production from maize.
Current opinion in biotechnology Jun, 2007 | Pubmed ID: 17399975
Low-dose exposure and immunogenicity of transgenic maize expressing the Escherichia coli heat-labile toxin B subunit.
Environmental health perspectives Mar, 2007 | Pubmed ID: 17431483
Compound leaf development and evolution in the legumes.
The Plant cell Nov, 2007 | Pubmed ID: 17993625
Persistence and degradation of maize-expressed vaccine protein, Escherichia coli heat-labile enterotoxin subunit B, in soil and water.
Environmental toxicology and chemistry Jun, 2008 | Pubmed ID: 18198938
Generation of transgenic maize with enhanced provitamin A content.
Journal of experimental botany , 2008 | Pubmed ID: 18723758
Subacute effects of maize-expressed vaccine protein, Escherichia coli heat-labile enterotoxin subunit B (LTB), on the Springtail, Folsomia candida , and the earthworm, Eisenia fetida.
Journal of agricultural and food chemistry Dec, 2008 | Pubmed ID: 19012409
Biolistic gun-mediated maize genetic transformation.
Methods in molecular biology (Clifton, N.J.) , 2009 | Pubmed ID: 19378004
A bacterial signal peptide is functional in plants and directs proteins to the secretory pathway.
Journal of experimental botany , 2009 | Pubmed ID: 19491306
Wet-milling transgenic maize seed for fraction enrichment of recombinant subunit vaccine.
Biotechnology progress Mar-Apr, 2010 | Pubmed ID: 19938061
Generation of backbone-free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome.
Plant physiology Mar, 2010 | Pubmed ID: 20023148
Utilizing protein-lean coproducts from corn containing recombinant pharmaceutical proteins for ethanol production.
Journal of agricultural and food chemistry Oct, 2010 | Pubmed ID: 20809624
Genetic transformation using maize immature zygotic embryos.
Methods in molecular biology (Clifton, N.J.) , 2011 | Pubmed ID: 21207278
Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase.
BMC biotechnology Jun, 2011 | Pubmed ID: 21702901
Parameters affecting the efficient delivery of mesoporous silica nanoparticle materials and gold nanorods into plant tissues by the biolistic method.
Small (Weinheim an der Bergstrasse, Germany) Feb, 2012 | Pubmed ID: 22174078
Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications.
Plant cell reports Apr, 2012 | Pubmed ID: 22179259
Ectopic expression of bacterial amylopullulanase enhances bioethanol production from maize grain.
Plant cell reports Sep, 2013 | Pubmed ID: 23652819
A genome-wide survey of highly expressed non-coding RNAs and biological validation of selected candidates in Agrobacterium tumefaciens.
PloS one , 2013 | Pubmed ID: 23950988
Genetic transformation of major cereal crops.
The International journal of developmental biology , 2013 | Pubmed ID: 24166432
Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision.
Plant physiology Feb, 2014 | Pubmed ID: 24376280
Proteolistics: a biolistic method for intracellular delivery of proteins.
Transgenic research Oct, 2014 | Pubmed ID: 25092532
Maize (Zea mays L.).
Methods in molecular biology (Clifton, N.J.) , 2015 | Pubmed ID: 25300834
Rice, Japonica (Oryza sativa L.).
Methods in molecular biology (Clifton, N.J.) , 2015 | Pubmed ID: 25300839
Soybean [Glycine max (L.) Merr].
Methods in molecular biology (Clifton, N.J.) , 2015 | Pubmed ID: 25300848
Heritable site-specific mutagenesis using TALENs in maize.
Plant biotechnology journal Sep, 2015 | Pubmed ID: 25644697
Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice.
Plant cell reports Jun, 2015 | Pubmed ID: 25677970
The Regulatory Status of Genome-edited Crops.
Plant biotechnology journal Feb, 2016 | Pubmed ID: 26251102
Assessment of ptxD gene as an alternative selectable marker for Agrobacterium-mediated maize transformation.
Plant cell reports May, 2016 | Pubmed ID: 26883223
Advancing Crop Transformation in the Era of Genome Editing.
The Plant cell Jul, 2016 | Pubmed ID: 27335450
An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize.
Plant biotechnology journal 02, 2017 | Pubmed ID: 27510362
Increasing seed size and quality by manipulating BIG SEEDS1 in legume species.
Proceedings of the National Academy of Sciences of the United States of America Nov, 2016 | Pubmed ID: 27791139
Nanoparticle-Mediated Recombinase Delivery into Maize.
Methods in molecular biology (Clifton, N.J.) , 2017 | Pubmed ID: 28815500
Agrobacterium- and Biolistic-Mediated Transformation of Maize B104 Inbred.
Methods in molecular biology (Clifton, N.J.) , 2018 | Pubmed ID: 28986902
RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels.
Planta Jun, 2018 | Pubmed ID: 29541880
Small Noncoding RNAs in Agrobacterium tumefaciens.
Current topics in microbiology and immunology , 2018 | Pubmed ID: 29556823
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize.
Plant biotechnology journal 02, 2019 | Pubmed ID: 29972722
An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize.
Methods in molecular biology (Clifton, N.J.) , 2019 | Pubmed ID: 30610633
CRISPR/Cas9-mediated targeted T-DNA integration in rice.
Plant molecular biology Mar, 2019 | Pubmed ID: 30645710
Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis.
BMC biology 01, 2019 | Pubmed ID: 30704461
Technological advances in maize breeding: past, present and future.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik Mar, 2019 | Pubmed ID: 30798332
High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice.
Scientific reports Dec, 2019 | Pubmed ID: 31882637
Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene.
Rice (New York, N.Y.) Jan, 2020 | Pubmed ID: 31965382
Alicia Masters1,2,
Minjeong Kang1,3,4,
Morgan McCaw1,3,
Jacob D. Zobrist1,3,5,
William Gordon-Kamm2,
Todd Jones2,
Kan Wang1,3
1Department of Agronomy, Iowa State University,
2Department of Applied Science and Technology, Corteva Agriscience,
3Crop Bioengineering Center, Iowa State University,
4Interdepartmental Plant Biology Major, Iowa State University,
5Interdepartmental Genetics and Genomics Major, Iowa State University
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