Methods Article

Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model

5.9K Views

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06:00 min

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June 30th, 2023

DOI :

10.3791/65513-v

June 30th, 2023

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Laura Charlès¹^,²^,³, Thomas Agius*⁴^,⁵, Irina Filz von Reiterdank*¹^,²^,³^,⁶, Janna Hagedorn¹^,²^,³, Yanis Berkane¹^,²^,³^,⁷, Hyshem H. Lancia¹^,²^,³, Basak E. Uygun²^,³^,⁸, Korkut Uygun²^,³^,⁸, Curtis L. Cetrulo Jr.¹^,²^,³^,⁹, Mark A. Randolph¹^,²^,³^,⁹, Alexandre G. Lellouch¹^,²^,³^,⁸

¹Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, ²Harvard Medical School, ³Shriners Children’s Boston, ⁴Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, ⁵Department of Vascular Surgery, Centre Hospitalier Universitaire Vaudois and University of Lausanne, ⁶Department of Plastic, Reconstructive and Hand Surgery, University Medical Center Utrecht, ⁷Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes), Rennes 1 University, ⁸Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, ⁹Plastic Surgery Research Laboratory, Massachusetts General Hospital

Transcript

Summary

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Blood Sampling

Rat Model

Lateral Tail Vein Puncture

Dorsal Penile Vein Puncture

Isoflurane

Vessel Spasm

Hematological Parameters

Experimental Animal Design

Blood Collection Techniques

Chapters in this video

0:00

Introduction

1:01

Tail Vein Puncture Without Anesthesia

4:10

Drawing Blood from the Penile Vein

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