Radboud University Nijmegen Medical Centre
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
Kirsty R. Short 1, Dimitri A. Diavatopoulos 2, Patrick C. Reading 1, Lorena E. Brown 1, Kelly L. Rogers 3, Richard A. Strugnell 1, Odilia L.C. Wijburg 1
1Department of Microbiology and Immunology, University of Melbourne, 2Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research
A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.
Immunology and Infection
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Marloes Vissers 1, Marrit N. Habets 1, Inge M. L. Ahout 1, Jop Jans 1, Marien I. de Jonge 1, Dimitri A. Diavatopoulos 1, Gerben Ferwerda 1
1Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud university medical center
Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection.
