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Radboud University Nijmegen Medical Centre

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Kirsty R. Short¹, Dimitri A. Diavatopoulos², Patrick C. Reading¹, Lorena E. Brown¹, Kelly L. Rogers³, Richard A. Strugnell¹, Odilia L.C. Wijburg¹

¹Department of Microbiology and Immunology, University of Melbourne, ²Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, ³The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research

A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.

Immunology and Infection

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Marloes Vissers¹, Marrit N. Habets¹, Inge M. L. Ahout¹, Jop Jans¹, Marien I. de Jonge¹, Dimitri A. Diavatopoulos¹, Gerben Ferwerda¹

¹Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud university medical center

Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection.