Universidad de Buenos Aires, CONICET

1 ARTICLES PUBLISHED IN JoVE

Biology

Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Felipe J. Renna¹, Malena Herrera Lopez¹, Maria Manifava², Nicholas T. Ktistakis², Maria I. Vaccaro¹

¹Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, ²Signalling Programme, The Babraham Institute

The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.