Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 ImmunofluorescenceFelipe J. Renna 1, Malena Herrera Lopez 1, Maria Manifava 2, Nicholas T. Ktistakis 2, Maria I. Vaccaro 1
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute
The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.
