Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

NAD(P)H Fluorescence Lifetime Imaging for the Metabolic Analysis of the Murine Intestine and Parasites During Nematode Infection

Wjatscheslaw Liublin^1,2, Sebastian Rausch³, Ruth Leben^1,2,3, Juliane Liebeskind³, Anja E. Hauser^4,5, Susanne Hartmann³, Raluca A. Niesner^2,1

¹Biophysical Analytics, Deutsches Rheuma-Forschungszentrum, Berlin - A Leibniz Institute, ²Dynamic and Functional In Vivo Imaging, Department of Veterinary Medicine, Institute for Veterinary Physiology, Freie Universität Berlin, ³Institute of Immunology, Department of Veterinary Medicine, Freie Universität Berlin, ⁴Laboratory for Immune Dynamics, Deutsches Rheuma-Forschungszentrum, Berlin - A Leibniz Institute, ⁵Intravital Microscopy and Immune Dynamics, Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin

The present protocol describes the NAD(P)H fluorescence lifetime imaging of an explanted murine intestine infected with the natural parasite Heligmosomoides polygyrus, which allows one to investigate metabolic processes both in host and parasite tissues in a spatially resolved manner.