NAD(P)H Fluorescence Lifetime Imaging for the Metabolic Analysis of the Murine Intestine and Parasites During Nematode InfectionWjatscheslaw Liublin 1,2, Sebastian Rausch 3, Ruth Leben 1,2,3, Juliane Liebeskind 3, Anja E. Hauser 4,5, Susanne Hartmann 3, Raluca A. Niesner 2,1
1Biophysical Analytics, Deutsches Rheuma-Forschungszentrum, Berlin - A Leibniz Institute, 2Dynamic and Functional In Vivo Imaging, Department of Veterinary Medicine, Institute for Veterinary Physiology, Freie Universität Berlin, 3Institute of Immunology, Department of Veterinary Medicine, Freie Universität Berlin, 4Laboratory for Immune Dynamics, Deutsches Rheuma-Forschungszentrum, Berlin - A Leibniz Institute, 5Intravital Microscopy and Immune Dynamics, Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin
The present protocol describes the NAD(P)H fluorescence lifetime imaging of an explanted murine intestine infected with the natural parasite Heligmosomoides polygyrus, which allows one to investigate metabolic processes both in host and parasite tissues in a spatially resolved manner.
