University of California Davis Health
2 ARTICLES PUBLISHED IN JoVE
Medicine
Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice
Yu-Yang Lin 1, Blaine A. Christiansen 1
1Lawrence J. Ellison Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of California Davis Health
Non-invasive ACL injury is a reliable and clinically relevant method for initiating post-traumatic osteoarthritis (PTOA) in mice. This injury method also allows for in vivo quantification of protease activity in the joint at early time points post-injury using protease-activatable near infrared probes and fluorescence reflectance imaging.
Biology
Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
Line O. Elingaard-Larsen 1,2, Katie L. Whytock 1, Adeline Divoux 1, Meghan Hopf 1, Erin E. Kershaw 3, Jamie N. Justice 4, Bret H. Goodpaster 1, Nancy E. Lane 5, Lauren M. Sparks 1
1Translational Research Institute, AdventHealth, 2Steno Diabetes Center Copenhagen, 3Division of Endocrinology and Metabolism, University of Pittsburgh, 4Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, 5Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health
The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.
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