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University of California Davis Health

2 ARTICLES PUBLISHED IN JoVE

Medicine

Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice

Yu-Yang Lin¹, Blaine A. Christiansen¹

¹Lawrence J. Ellison Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of California Davis Health

Non-invasive ACL injury is a reliable and clinically relevant method for initiating post-traumatic osteoarthritis (PTOA) in mice. This injury method also allows for in vivo quantification of protease activity in the joint at early time points post-injury using protease-activatable near infrared probes and fluorescence reflectance imaging.

Biology

Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

Line O. Elingaard-Larsen^1,2, Katie L. Whytock¹, Adeline Divoux¹, Meghan Hopf¹, Erin E. Kershaw³, Jamie N. Justice⁴, Bret H. Goodpaster¹, Nancy E. Lane⁵, Lauren M. Sparks¹

¹Translational Research Institute, AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism, University of Pittsburgh, ⁴Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health

The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.