State University of Santa Cruz - UESC
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages
Uener Ribeiro dos Santos *1, Julyanna Oliveira de Castro *1, Melissa Ercília Santos Matos 1, Giulia De Bonis 2, Jane Lima dos Santos 1
1Laboratory of Immunobiology - Department of Biological Sciences, State University of Santa Cruz - UESC, 2Università Degli Studi di Genova
The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.
Biology
Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue
Marianna Gabrielle Almeida Galvão 1, Brisa Miranda Andrade Santos 1, Cahê Moreira Aguiar 2, Adriana Bozzi 1,2
1Laboratory of Immunobiology, Department of Biological Sciences, State University of Santa Cruz - UESC, 2Department of Medicine, Afya Medical Sciences College
The adipose tissue is an excellent source of mesenchymal stem cells. Here, we bring the step-by-step extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from Swiss mice epididymal adipose tissue.
