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State University of Santa Cruz - UESC

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

Uener Ribeiro dos Santos^*1, Julyanna Oliveira de Castro^*1, Melissa Ercília Santos Matos¹, Giulia De Bonis², Jane Lima dos Santos¹

¹Laboratory of Immunobiology - Department of Biological Sciences, State University of Santa Cruz - UESC, ²Università Degli Studi di Genova

The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.

Biology

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Marianna Gabrielle Almeida Galvão¹, Brisa Miranda Andrade Santos¹, Cahê Moreira Aguiar², Adriana Bozzi^1,2

¹Laboratory of Immunobiology, Department of Biological Sciences, State University of Santa Cruz - UESC, ²Department of Medicine, Afya Medical Sciences College

The adipose tissue is an excellent source of mesenchymal stem cells. Here, we bring the step-by-step extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from Swiss mice epididymal adipose tissue.