Sorbonne University
2 ARTICLES PUBLISHED IN JoVE
Medicine
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
Raïko Blondonnet 1,2, Bertille Paquette 1,2, Damien Richard 3, Rémi Bourg 2,4, Géraldine Laplace 2,4, Romain Segurel 2,4, Henria Pouvelle 2,4, Corinne Belville 2, Loic Blanchon 2, Thomas Godet 1,2, Jean-Michel Constantin 1,2, Jean-Etienne Bazin 1,2, Vincent Sapin 2, Matthieu Jabaudon 1,2
1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, 3Department of Pharmacology, CHU Clermont-Ferrand, 4Nurse Anesthetist School, CHU Clermont-Ferrand
We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.
JoVE Core
Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device
Raiko Blondonnet 1,2, Bertille Paquette 1,2, Jules Audard 1,2, Ridvan Guler 1,2, François-Xavier Roman 1,2, Ruoyang Zhai 2, Corinne Belville 2, Loïc Blanchon 2, Thomas Godet 1, Emmanuel Futier 1,2, Jean-Etienne Bazin 1, Jean-Michel Constantin 3, Vincent Sapin 2,4, Matthieu Jabaudon 1,2,5
1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2GReD, CNRS, INSERM, Université Clermont Auvergne, 3GRC 29, AP-HP, DMU DREAM, Department of Anesthesiology and Critical Care, Pitié-Salpêtrière Hospital, Sorbonne University, 4Department of Biochemistry and Molecular Genetics, CHU Clermont-Ferrand, 5Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center
We describe a model of hydrochloric acid-induced acute respiratory distress syndrome (ARDS) in piglets receiving sedation with halogenated agents, isoflurane and sevoflurane, through a device used for inhaled intensive care sedation. This model can be used to investigate the biological mechanisms of halogenated agents on lung injury and repair.
