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Sorbonne University

2 ARTICLES PUBLISHED IN JoVE

Medicine

In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

Raïko Blondonnet^1,2, Bertille Paquette^1,2, Damien Richard³, Rémi Bourg^2,4, Géraldine Laplace^2,4, Romain Segurel^2,4, Henria Pouvelle^2,4, Corinne Belville², Loic Blanchon², Thomas Godet^1,2, Jean-Michel Constantin^1,2, Jean-Etienne Bazin^1,2, Vincent Sapin², Matthieu Jabaudon^1,2

¹Department of Perioperative Medicine, CHU Clermont-Ferrand, ²Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, ³Department of Pharmacology, CHU Clermont-Ferrand, ⁴Nurse Anesthetist School, CHU Clermont-Ferrand

We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.

JoVE Core

Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device

Raiko Blondonnet^1,2, Bertille Paquette^1,2, Jules Audard^1,2, Ridvan Guler^1,2, François-Xavier Roman^1,2, Ruoyang Zhai², Corinne Belville², Loïc Blanchon², Thomas Godet¹, Emmanuel Futier^1,2, Jean-Etienne Bazin¹, Jean-Michel Constantin³, Vincent Sapin^2,4, Matthieu Jabaudon^1,2,5

¹Department of Perioperative Medicine, CHU Clermont-Ferrand, ²GReD, CNRS, INSERM, Université Clermont Auvergne, ³GRC 29, AP-HP, DMU DREAM, Department of Anesthesiology and Critical Care, Pitié-Salpêtrière Hospital, Sorbonne University, ⁴Department of Biochemistry and Molecular Genetics, CHU Clermont-Ferrand, ⁵Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center

We describe a model of hydrochloric acid-induced acute respiratory distress syndrome (ARDS) in piglets receiving sedation with halogenated agents, isoflurane and sevoflurane, through a device used for inhaled intensive care sedation. This model can be used to investigate the biological mechanisms of halogenated agents on lung injury and repair.