Shanghai-MOST Key Laboratory of Health and Disease Genomics

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Jianan Tang^1,2,3, Baoli Zhang^1,2,3, Chunhua Tang^4,5,6, Xuemei Wang^1,2,3, Minmin Hua^1,2,3, Yuchuan Zhou^4,5,6, Huijuan Shi^1,2,3, Tiancheng Zhang^1,2,3

¹Shanghai-MOST Key Laboratory of Health and Disease Genomics, ²NHC Key Lab of Reproduction Regulation, ³Shanghai Institute for Biomedical and Pharmaceutical Technologies, ⁴International Peace Maternity and Child Health Hospital, ⁵School of Medicine, Shanghai Jiao Tong University, ⁶Shanghai Key Laboratory of Embryo Original Diseases

Here, we present a protocol to establish an intracytoplasmic sperm injection (ICSI)-embryo transfer (ET) mouse model, allowing us to observe age-related changes in glucose metabolism that may be attributed to ICSI, providing insights into its potential long-term impacts on human development.