Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program, Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program, National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaén

This protocol presents a detailed methodological framework for electroporation-based transgenesis of cardiac cells in developing mouse hearts. The video assets provided here will facilitate learning of this versatile technique.

Medicine

Real-Time Cardiac Mapping with a Noninvasive Imageless Electrocardiographic Imaging System

Jana Reventos-Presmanes^1,2,3, Carlos Fambuena^2,3, Andrea Cano-Cabañero², Maria Correas², Ismael Hernández-Romero^2,3, Clara Herrero-Martin², David Lundback^2,3, Ines Llorente², Marta Martinez², Raúl Moreno-Lopez², Rubén Molero^2,3, Javier Milagro³, Berta Pellicer-Sendra^1,3, Mariona Regany¹, Santiago Ros^2,4, Jaume Serrano^1,3, Jorge Vicente³, Ernesto Zacur³, Till Althoff¹, Elena Arbelo^1,5,6, Angel Arenal⁴, Pablo Ávila⁴, Alejandro Carta-Bergaz⁴, Eduard Guasch^1,5,6, Jean-Baptiste Guichard¹, Maite Izquierdo⁷, Margarida Pujol-López^1,5, Andreu Porta-Sánchez^1,5,6, Ivo Roca-Luque^1,5,6, José M. Tolosana^1,5,6, Esteban G. Torrecilla⁴, Joaquin Osca⁷, Lluís Mont^1,5,6, Felipe Atienza^3,4, Maria S. Guillem^2,3, Andreu M. Climent^2,3

¹Arrhythmia Section, Institut Clínic Cardiovascular, Hospital Clínic, Universitat de Barcelona, ²ITACA Institute, Universitat Politècnica de València, ³Corify Care SL, ⁴Arrhythmia Section, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), ⁵Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), ⁶Centro de Investigación Biomédica En Red de enfermedades CardioVasculares (CIBERCV), ⁷Arrhythmia Section, Hospital Universitari i Politècnic La Fe

This study introduces a novel approach for real-time cardiac mapping using a noninvasive, imageless electrocardiographic imaging system. This system enables the acquisition of electrophysiological cardiac maps without requiring pre-procedural computed tomography or magnetic resonance imaging scans, enabling efficient guidance for cardiac procedures such as ablation and cardiac resynchronization therapy implants.