An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic HeartJorge Mañes-García 1, Leonardo Beccari 1, Miguel Torres 2,3, Oscar H. Ocaña 2,4
1Tissue and Organ Homeostasis Program, Severo Ochoa Center for Molecular Biology (CBMSO), 2Cardiovascular Regeneration Program, National Cardiovascular Research Center (CNIC), 3Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), 4Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaén
This protocol presents a detailed methodological framework for electroporation-based transgenesis of cardiac cells in developing mouse hearts. The video assets provided here will facilitate learning of this versatile technique.
