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Binzhou Medical University Hospital

1 ARTICLES PUBLISHED IN JoVE

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Medicine

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium
Anqi He *1,2, Song Wang *1,2,3, Kaiyu Li 1,2, Baosong Li 1,2,4, Wanyi Xiao 1,2, Gang Liu 1,2
1Department of General Surgery, Tianjin Medical University General Hospital, 2Tianjin General Surgery Institute, 3Department of General Surgery, Zibo Municipal Hospital, 4Department of General Surgery, Binzhou Medical University Hospital

This protocol describes the method for establishing a rat model of pouchitis. The ileal pouch model was created by performing ileal pouch-anal anastomosis (IPAA) surgery using microsurgical techniques. After the surgery, the rat was treated with 4% dextran sulfate sodium (DSS) for 4 days.

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