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Binzhou Medical University Hospital

1 ARTICLES PUBLISHED IN JoVE

Medicine

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium

Anqi He^*1,2, Song Wang^*1,2,3, Kaiyu Li^1,2, Baosong Li^1,2,4, Wanyi Xiao^1,2, Gang Liu^1,2

¹Department of General Surgery, Tianjin Medical University General Hospital, ²Tianjin General Surgery Institute, ³Department of General Surgery, Zibo Municipal Hospital, ⁴Department of General Surgery, Binzhou Medical University Hospital

This protocol describes the method for establishing a rat model of pouchitis. The ileal pouch model was created by performing ileal pouch-anal anastomosis (IPAA) surgery using microsurgical techniques. After the surgery, the rat was treated with 4% dextran sulfate sodium (DSS) for 4 days.