Ehime University
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
Wei Zhou 1, Hiroyuki Takeda 2
1Graduate School of Medicine, Ehime University, 2Proteo-Science Center, Ehime University
This protocol describes an efficient cell-free method for production of high-quality proteoliposome by bilayer-dialysis method using wheat cell-free system and liposomes. This method provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.
Biology
Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
Noritaka Saeki 1,2, Yuuki Imai 2,3
1Division of Medical Research Support, Advanced Research Support Center, Ehime University, 2Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, 3Department of Pathophysiology, Graduate School of Medicine, Ehime University
The present study provides a modified protocol to isolate synovial macrophages and fibroblasts from murine inflammatory arthritis tissue.
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