Accedi

Ehime University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

¹Graduate School of Medicine, Ehime University, ²Proteo-Science Center, Ehime University

This protocol describes an efficient cell-free method for production of high-quality proteoliposome by bilayer-dialysis method using wheat cell-free system and liposomes. This method provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.

Biology

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Noritaka Saeki^1,2, Yuuki Imai^2,3

¹Division of Medical Research Support, Advanced Research Support Center, Ehime University, ²Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, ³Department of Pathophysiology, Graduate School of Medicine, Ehime University

The present study provides a modified protocol to isolate synovial macrophages and fibroblasts from murine inflammatory arthritis tissue.