IRCCS Ospedale San Raffaele

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Biology

Setting a Successful Sorting for Extracellular Vesicle Isolation
Monica Romanò *1, Daniela Boselli *1, Enrico Ragni 2, Laura de Girolamo 2, Chiara Villa 1,3
1FRACTAL - Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory, IRCCS Ospedale San Raffaele, 2Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico Galeazzi, 3Università Vita-Salute San Raffaele

This protocol provides a detailed description of sorting extracellular vesicles (EVs) released by mesenchymal stromal cells. In particular, it focuses on the instrument setting and the optimization of the sorting conditions. The goal is to sort extracellular vesicles while preserving their characteristics.

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