IRCCS Ospedale San Raffaele

1 ARTICLES PUBLISHED IN JoVE

Biology

Setting a Successful Sorting for Extracellular Vesicle Isolation

Monica Romanò^*1, Daniela Boselli^*1, Enrico Ragni², Laura de Girolamo², Chiara Villa^1,3

¹FRACTAL - Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory, IRCCS Ospedale San Raffaele, ²Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico Galeazzi, ³Università Vita-Salute San Raffaele

This protocol provides a detailed description of sorting extracellular vesicles (EVs) released by mesenchymal stromal cells. In particular, it focuses on the instrument setting and the optimization of the sorting conditions. The goal is to sort extracellular vesicles while preserving their characteristics.