Tufts University Medical Center

1 ARTICLES PUBLISHED IN JoVE

Biology

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Abrahim I. Orabi¹, Kamaldeen A. Muili¹, Dong Wang¹, Shunqian Jin¹, George Perides², Sohail Z. Husain¹

¹Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, ²Department of Surgery, Tufts University Medical Center

We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.