Babraham institute
2 ARTICLES PUBLISHED IN JoVE
Genetics
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
Laetitia Chauve *1, Jérémie Le Pen *2,3,5, Francesca Hodge 1, Pia Todtenhaupt 1, Laura Biggins 1, Eric A. Miska 2,3,4, Simon Andrews 1, Olivia Casanueva 1
1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University
In this article a high-throughput protocol for fast and reliable determination of gene expression levels in single or bulk C. elegans samples is described. This protocol does not require RNA isolation and produces cDNA directly from samples. It can be used together with high-throughput multiplexed nanofluidic real-time qPCR platforms.
Biology
Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans
Hanneke Okkenhaug 1, Laetitia Chauve 1, Fatemeh Masoudzadeh 1, Lars Okkenhaug 1, Olivia Casanueva 1
1Babraham Institute
Worm-align/Worm_CP is a simple FIJI/CellProfiler workflow that can be used to straighten and align Caenorhabditis elegans samples and to score whole-worm image-based assays without the need for prior training steps. We have applied Worm-align/Worm_CP to the quantification of heat-shock induced expression in live animals or lipid droplets in fixed samples.
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