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Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

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11:16 min

May 28th, 2020

May 28th, 2020

5,834 Views

0:05

Introduction

0:57

Mounting Fixed Worms for Imaging

2:56

Creating Montage Images of Aligned Single Worms Using the Worm-align FIJI Pipeline

6:01

Analyzing Single-worm Fluorescence Intensity Using the Output from Worm-align in an Automated CellProfiler Pipeline

8:07

Results: Fluorescence Quantification with the Worm_CP Pipeline

9:50

Conclusion

副本

Worm-align/Worm_CP is a simple FIJI/CellProfiler workflow that can be used to straighten and align Caenorhabditis elegans samples and to score whole-worm image-based assays without the need for prior training steps. We have applied Worm-align/Worm_CP to the quantification of heat-shock induced expression in live animals or lipid droplets in fixed samples.

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